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AB272522

Chromium Assay Kit

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Chromium Assay Kit (ab272522) provides a simple, one-step colorimetric means to directly measure Cr(VI) in a sample.
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Functional Studies - Chromium Assay Kit (AB272522)
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Supplier Data

Functional Studies - Chromium Assay Kit (AB272522)

Typical Standard Curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Example of Chromium Assay Kit standard curves.

Key facts

Detection method

Colorimetric

Sample types

Food samples, Plasma, Serum

Results type

Quantitative

Range

20 - 2000 µg/L

Assay Platform

Microplate

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Product details

Chromium Assay Kit (ab272522) provides a simple, one-step colorimetric means to directly measure Cr(VI) in a sample. In the assay, Cr(VI) forms a stable complex with a specific chromogenic dye. The optical density at 480nm is directly proportionate to the Cr(VI) concentration in the sample. Cr(III) can be converted to Cr(VI) with nitric acid/hydrochloric acid, thus allowing the determination of Cr(III) or total Cr [Cr(III) + Cr(VI)] in the sample. The assay is sensitive with a detection limit of 20 μg/L Cr.

Sensitive and accurate. Linear detection range of 20 - 2000 μg/L Chromium.

Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated for processing thousands of samples per day.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "250Test": { "sellingSize": "250 Test", "publicAssetCode":"ab272522-250Test", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Reagent B", "number":"AB272522-CMP02", "productcode":"" }, { "size":"1 x 300 µL", "name":"Reagent A", "number":"AB272522-CMP01", "productcode":"" }, { "size":"1 x 300 µL", "name":"Cr (VI) Standard", "number":"AB272522-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Chromium is a transition metal with the atomic number 24 and an approximate atomic mass of 51.9961 u. It is often found in trace amounts in various biological systems. In laboratories chromium specifically in the form of radioactive chromium-51 acts as a tracer in hematological assays such as chromium release assays and CR release assays. Chromium can form part of a kit often utilized in immunoassays for labeling cells and studying cellular functions.
Biological function summary

Chromium plays a role in glucose metabolism and lipid regulation. It is recognized as a component of chromodulin a protein complex that may enhance insulin signaling and improve glucose uptake in cells. The protein resides within tissues such as liver kidney and adipose tissues. Adequate chromium levels in the body are associated with improved insulin sensitivity and carbohydrate metabolism.

Pathways

Chromium's influence on insulin signaling affects various metabolic pathways including gluconeogenesis and the insulin signaling pathway. These pathways involve important proteins like insulin receptor substrates and GLUT4 which contribute to glucose transport and energy homeostasis. Although chromium doesn’t directly bind to these proteins it modifies their activity via its involvement in the insulin pathway.

Chromium deficiency associates with Type 2 diabetes and dyslipidemia. Inadequate chromium levels lead to impaired glucose tolerance and altered lipid profiles. Chromium supplementation consequently shows benefits in restoring normal insulin and glucose function. The protein insulin receptor substrate 1 (IRS-1) is particularly relevant as chromium's biological role ties into its modulatory effect on insulin action therefore impacting IRS-1-mediated signaling.
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Product protocols

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Target data

See full target information Chromium
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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