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AB83396

Citrate Assay Kit

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(27 Publications)

Citrate Assay Kit ab83396 provides a simple, sensitive and rapid means of quantifying citrate in biological samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Functional Studies - Citrate Assay Kit (AB83396)
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Lab

Functional Studies - Citrate Assay Kit (AB83396)

Functional assay - 83396 Citrate Assay Kit.

Citrate measured fluorimetrically in various biofluids showing concentration (micromolar).

Functional Studies - Citrate Assay Kit (AB83396)
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Supplier Data

Functional Studies - Citrate Assay Kit (AB83396)

Citrate standard curve generated using this kit protocol

Functional Studies - Citrate Assay Kit (AB83396)
  • FuncS

Lab

Functional Studies - Citrate Assay Kit (AB83396)

Functional assay - 83396 Citrate Assay Kit.

Fluorimetric standard curve : mean of duplicates (+/-SD) with background readings subtracted.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum

Assay type

Quantitative

Sensitivity

> 0.002 mM

Range

0.002 - 10 mM

Assay time

40m

Assay Platform

Microplate reader

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Product details

Citrate Assay Kit ab83396 provides a simple, sensitive and rapid means of quantifying citrate in biological samples.

In the citrate assay protocol, citrate is converted to pyruvate via oxaloacetate. The pyruvate is quantified by converting a nearly colorless probe to an intensely colored (570 nm) and fluorescent (Ex/Em, 535/587 nm) product.

The citrate assay kit can detect 0.1 to 10 nmoles (~2 uM-10 mM) of citrate.

Citrate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader

Other Notes
This product was previously called K655 Citrate Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

Citric acid (HOOC-CH2-C(-OH)(-COOH)-CH2-COOH) is a key intermediate in the TCA cycle which occurs in mitochondria. It is formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA derived from the glycolytic pathway. Citrate can be transported out of mitochondria and converted back to acetyl CoA for fatty acid synthesis.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab83396-100Test", "assetComponentDetails": [ { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB83396-CMP01", "productcode":"AB309437" }, { "size":"1 x 1 Vial", "name":"Citrate Standard", "number":"AB83396-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Citrate Enzyme Mix", "number":"AB83396-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Citrate Developer", "number":"AB83396-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"Citrate Assay Buffer", "number":"AB83396-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Citrate also known as citric acid is a tricarboxylic acid with the molecular mass of approximately 192.13 g/mol. It is a small molecule that plays a critical role in metabolism. Citrate exists extensively in the mitochondria of eukaryotic cells and in the cytosol. Its high abundance in these locations allows it to participate in various biochemical reactions necessary for cellular energy production. Citrate forms through the condensation of acetyl-CoA and oxaloacetate by the enzyme citrate synthase initiating the tricarboxylic acid (TCA) cycle also known as the Krebs cycle.
Biological function summary

The presence of citrate is essential in the regulation of the TCA cycle where it functions as an important intermediate. Among its functions citrate can inhibit the enzyme phosphofructokinase-1 linking glycolysis and the TCA cycle. This inhibition ensures a balance between the supply of glucose and the demand for ATP. Citrate does not form part of a complex but it interacts with various metabolic enzymes establishing its role as a metabolic regulator. Additionally citrate acts as a substrate for ATP-citrate lyase aiding in the production of acetyl-CoA in the cytosol vital for fatty acid synthesis.

Pathways

Citrate involves itself significantly in the TCA cycle and fatty acid synthesis. The TCA cycle a central component of cellular respiration generates reducing equivalents for oxidative phosphorylation by processing acetyl-CoA. As citrate is part of this pathway it interacts with enzymes such as aconitase and isocitrate dehydrogenase. In fatty acid synthesis citrate serves as an acetyl-CoA donor after being converted by ATP-citrate lyase. This conversion links citrate to important pathways such as lipogenesis and cholesterol synthesis highlighting its diverse roles in cellular metabolism.

Citrate has a significant relationship with conditions like cancer and metabolic syndrome. Increased citrate production or its accumulation can occur in cancer cells as tumors often exhibit altered metabolism known as the Warburg effect. In these cases regulatory proteins like hypoxia-inducible factor (HIF) can affect citrate metabolism. In metabolic syndrome altered citrate levels can contribute to disrupted metabolic pathways impacting proteins such as acetyl-CoA carboxylase and fatty acid synthase. Understanding citrate's function in these diseases potentially offers insights into therapeutic targets.
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Product protocols

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Target data

See full target information Citrate
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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 116:152-163 PubMed39479926

2024

Noncanonical TCA cycle fosters canonical TCA cycle and mitochondrial integrity in acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Atsushi Watanabe,Chartsiam Tipgomut,Haruhito Totani,Kentaro Yoshimura,Tomohiko Iwano,Hamed Bashiri,Lee Hui Chua,Chong Yang,Toshio Suda

Antioxidants (Basel, Switzerland) 13: PubMed39199131

2024

Citrate Promotes Nitric Oxide Production during Human Sperm Capacitation.

Applications

Unspecified application

Species

Unspecified reactive species

Diego Loggia,Cristian O'Flaherty

Clinical and translational medicine 14:e1785 PubMed39090662

2024

CPT1A-IL-10-mediated macrophage metabolic and phenotypic alterations ameliorate acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Muyun Wang,Di Wu,Ximing Liao,Haiyang Hu,Jing Gao,Linlin Meng,Feilong Wang,Wujian Xu,Shaoyong Gao,Jing Hua,Yuanyuan Wang,Qiang Li,Kun Wang,Wei Gao

Islets 16:2339558 PubMed38607959

2024

β cell acetate production and release are negligible.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Xu,Chioma Nnyamah,Nupur Pandya,Nadia Sweis,Irene Corona-Avila,Medha Priyadarshini,Barton Wicksteed,Brian T Layden

Molecular oncology 18:2212-2233 PubMed38425123

2024

Vitamin-C-dependent downregulation of the citrate metabolism pathway potentiates pancreatic ductal adenocarcinoma growth arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Aiora Cenigaonandia-Campillo,Ana Garcia-Bautista,Anxo Rio-Vilariño,Arancha Cebrian,Laura Del Puerto,José Antonio Pellicer,José Antonio Gabaldón,Horacio Pérez-Sánchez,Miguel Carmena-Bargueño,Carolina Meroño,Javier Traba,María Jesús Fernandez-Aceñero,Natalia Baños-Herraiz,Lorena Mozas-Vivar,Estrella Núñez-Delicado,Jesús Garcia-Foncillas,Óscar Aguilera

Cancer communications (London, England) 44:47-75 PubMed38133457

2023

Glucose-mediated mitochondrial reprogramming by cholesterol export at TM4SF5-enriched mitochondria-lysosome contact sites.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Eon Kim,So-Young Park,Chulhwan Kwak,Yoonji Lee,Dae-Geun Song,Jae Woo Jung,Haesong Lee,Eun-Ae Shin,Yangie Pinanga,Kyung-Hee Pyo,Eun Hae Lee,Wonsik Kim,Soyeon Kim,Chang-Duck Jun,Jeanho Yun,Sun Choi,Hyun-Woo Rhee,Kwang-Hyeon Liu,Jung Weon Lee

Life science alliance 7: PubMed38081642

2023

tRF-1:30-Gly-CCC-3 inhibits thyroid cancer via binding to PC and modulating metabolic reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Bifei Fu,YuMing Lou,Xiaofeng Lu,Zhaolin Wu,Junjie Ni,Cong Jin,Pu Wu,Chaoyang Xu

Communications biology 6:926 PubMed37689798

2023

The citrate transporters SLC13A5 and SLC25A1 elicit different metabolic responses and phenotypes in the mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Gonzalo Fernandez-Fuente,Katherine A Overmyer,Alexis J Lawton,Ildiko Kasza,Samantha L Shapiro,Patricia Gallego-Muñoz,Joshua J Coon,John M Denu,Caroline M Alexander,Luigi Puglielli

Journal of the European Academy of Dermatology and Venereology : JEADV 37:2067-2079 PubMed37247195

2023

Recombinant Treponema pallidum protein Tp47 promoted the phagocytosis of macrophages by activating NLRP3 inflammasome induced by PKM2-dependent glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Y-W Zheng,M Wang,J-W Xie,R Chen,X-T Wang,Y He,T-C Yang,L-L Liu,L-R Lin

Cell reports 42:112155 PubMed36857182

2023

Limiting Mrs2-dependent mitochondrial Mg uptake induces metabolic programming in prolonged dietary stress.

Applications

Unspecified application

Species

Unspecified reactive species

Travis R Madaris,Manigandan Venkatesan,Soumya Maity,Miriam C Stein,Neelanjan Vishnu,Mridula K Venkateswaran,James G Davis,Karthik Ramachandran,Sukanthathulse Uthayabalan,Cristel Allen,Ayodeji Osidele,Kristen Stanley,Nicholas P Bigham,Terry M Bakewell,Melanie Narkunan,Amy Le,Varsha Karanam,Kang Li,Aum Mhapankar,Luke Norton,Jean Ross,M Imran Aslam,W Brian Reeves,Brij B Singh,Jeffrey Caplan,Justin J Wilson,Peter B Stathopulos,Joseph A Baur,Muniswamy Madesh
View all publications
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