Citrate Synthase Activity Assay Kit (ab119692) is used to quantify mitochondrial citrate synthase (CS) activity.
- Antibody captures citrate synthase on plate, followed by treatment with enzyme substrate
- Readout on any colorimetric (412 nm) plate reader
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- Disease models & mechanisms 16:2023Increased cysteine metabolism in PINK1 models of Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarco Travaglio,Filippos Michopoulos,Yizhou Yu,Rebeka Popovic,Edmund Foster,Muireann Coen,L Miguel MartinsPubMed 36695500
- Frontiers in endocrinology 13:8955932022plays an important role in the pathogenesis of metabolic-associated fatty liver disease by regulating hepatic lipid metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongqiang Ma,Guangshun Chen,Junfang Yi,Qiang Li,Zhi Tan,Wenling Fan,Xiaohua Luo,Zhiyong He,Zhongzhou Si,Jiequn LiPubMed 35957832
- Journal of dairy science 105:7036-70462022Peripheral blood mononuclear cell mitochondrial enzyme activity is associated with parity and lactation performance in early lactation Holstein dairy cows.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA M Niesen,O N Genther-Schroeder,C M K Bradley,J A Davidson,H A RossowPubMed 35787326
- ACS bio & med chem Au 2:282-2962022Discovery of JND003 as a New Selective Estrogen-Related Receptor α Agonist Alleviating Nonalcoholic Fatty Liver Disease and Insulin Resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiufeng Mao,Lijie Peng,Xiaomei Ren,Yi Chu,Tao Nie,Wanhua Lin,Xuemei Zhao,Andrew Libby,Yong Xu,Yu Chang,Chong Lei,Kerry Loomes,Na Wang,Jinsong Liu,Moshe Levi,Donghai Wu,Xiaoyan Hui,Ke DingPubMed 35874496
- Oxidative medicine and cellular longevity 2021:82400152021Silencing LINC00294 Restores Mitochondrial Function and Inhibits Apoptosis of Glioma Cells under Hypoxia via the miR-21-5p/CASKIN1/cAMP Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaogang Dong,Qin Pi,Anwaier Yuemaierabola,Wenjia Guo,Hailong TianPubMed 34777696
- European journal of applied physiology 122:93-1022021Peak fat oxidation is positively associated with vastus lateralis CD36 content, fed-state exercise fat oxidation, and endurance performance in trained males.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEd Maunder,Daniel J Plews,Gareth A Wallis,Matthew J Brick,Warren B Leigh,Wee-Leong Chang,Tom Stewart,Casey M Watkins,Andrew E KildingPubMed 34562114
- Oxidative medicine and cellular longevity 2021:99579082021Citrate Synthase and OGDH as Potential Biomarkers of Atherosclerosis under Chronic Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing-Bing Meng,Gai-Feng Hu,Meng-Jie Shan,Yuan-Meng Zhang,Ze-Mou Yu,Yun-Qing Liu,Hong-Xuan Xu,Li Wang,Tao Gong,De-Ping LiuPubMed 34539976
- Physiological reports 9:e148492021Temperate performance and metabolic adaptations following endurance training performed under environmental heat stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEd Maunder,Daniel J Plews,Gareth A Wallis,Matthew J Brick,Warren B Leigh,Wee-Leong Chang,Casey M Watkins,Andrew E KildingPubMed 33977674
- Molecular cell 81:1905-1919.e122021Loss of LUC7L2 and U1 snRNP subunits shifts energy metabolism from glycolysis to OXPHOS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexis A Jourdain,Bridget E Begg,Eran Mick,Hardik Shah,Sarah E Calvo,Owen S Skinner,Rohit Sharma,Steven M Blue,Gene W Yeo,Christopher B Burge,Vamsi K MoothaPubMed 33852893
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts
- Assay type
- Enzyme activity
- Reactive species
- Cow, Human
- Range
- 1.56 - 100 µg/mL
- Assay time
- 3h 15m
- Sensitivity
- = 1.56 µg/mL
Associated Products
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Target data
Function
Key enzyme of the Krebs tricarboxylic acid cycle which catalyzes the synthesis of citrate from acetyl coenzyme A and oxaloacetate.
Alternative names
Citrate (Si)-synthase, CS
What's included?
Recommended products
Citrate Synthase Activity Assay Kit (ab119692) is used to quantify mitochondrial citrate synthase (CS) activity.
- Antibody captures citrate synthase on plate, followed by treatment with enzyme substrate
- Readout on any colorimetric (412 nm) plate reader
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts
- Assay type
- Enzyme activity
- Reactive species
- Cow, Human
- Range
- 1.56 - 100 µg/mL
- Assay time
- 3h 15m
- Assay Platform
- Microplate reader
- Sensitivity
- = 1.56 µg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Sample 1 | n 3 | C.V. 5.45 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Sample 1 | n 3 | C.V. 8.3 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Citrate Synthase Activity Assay Kit (ab119692) is used to determine mitochondrial citrate synthase (CS) activity in a sample. The enzyme is immunocaptured within the wells of a microplate, and activity is determined by recording color development of TNB, which is generated in the reaction of citrate synthesis. The reaction product, TNB, absorbs at OD = 412 nm.
This kit immunocaptures only native citrate synthase from the samples. Using the immunocapture method allows the measurement of citrate synthase activity with simple sample preparation and no need for mitochondrial isolation.
This kit can be used with whole cell extracts or tissue homogenates.
Citrate synthase assay protocol summary
- add samples to wells
- incubate for 3 hrs to capture enzyme
- wash with wash buffer
- add activity solution
- analyze with microplate reader for 5-30 min at 20s intervals
Related and recommended products
For a citrate synthase assay without the antibody pulldown step, see Citrate synthase assay kit Citrate Synthase Assay Kit ab239712.
Other Notes
Citrate synthase (CS, O75390) is the initial enzyme of the tricarboxylic acid (TCA) cycle. This enzyme is a 51.7 kDa enzyme that catalyzes the reaction of 2 carbon acetyl CoA with 4 carbon oxaloacetate to form the 6 carbon citrate (EC 2.3.3.1). This enzyme is an exclusive marker of the mitochondrial matrix. Citrate synthase is found in nearly all cells capable of oxidative metabolism.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Citrate synthetase also known as citrate synthase is an important enzyme in the tricarboxylic acid cycle. It catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and CoA. The enzyme weighs around 49 kilodaltons. It is expressed prominently in the mitochondria of eukaryotic cells where it initiates the Krebs cycle by providing citrate to be further processed. Citrate synthetase is an important point of control within this metabolic cycle.
- Biological function summary
Citrate synthetase plays a central role in energy production by converting oxaloacetate and acetyl-CoA into citrate. This enzyme is not known to be part of any larger complex but associates with downstream enzymes such as aconitase in the metabolic pathway. Its activity is essential for cellular respiration impacting the overall metabolic rate of the organism. The concentration of citrate produced serves as a checkpoint both for the continuation of the Krebs cycle and for feedback inhibition of glycolysis.
- Pathways
Citrate synthetase is integral to the Krebs cycle and the related oxidative phosphorylation pathway. These pathways are essential for efficient ATP production. Citrate synthetase works closely with enzymes like isocitrate dehydrogenase and alpha-ketoglutarate dehydrogenase within the Krebs cycle. Through these interactions citrate synthetase ensures the proper flow of carbon through the cycle impacting ATP yield and cellular energy homeostasis.
- Associated diseases and disorders
Citrate synthetase's function can influence metabolic diseases like diabetes and mitochondrial disorders. Alterations in its activity may contribute to the dysregulation of glucose metabolism seen in diabetes affecting enzymes like glucose transporter 4 (GLUT4). In mitochondrial disorders changes in its normal activity can cause energy production deficiencies influencing proteins such as cytochrome c which is critical in the electron transport chain. Understanding these interactions can help develop therapeutic strategies targeting metabolic pathways.
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2 product images
ELISA - Citrate Synthase Activity Assay Kit (ab119692)
Figure 1. Example activity measurements from titrated HepG2 cells.
ELISA - Citrate Synthase Activity Assay Kit (ab119692)
Figure 2. Example activity measurements from titrated of Human heart homogenate.
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- Visit troubleshooting
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