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AB119692

Citrate Synthase Activity Assay Kit

5

(1 Review)

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(35 Publications)

Citrate Synthase Activity Assay Kit (ab119692) is used to quantify mitochondrial citrate synthase (CS) activity.

- Antibody captures citrate synthase on plate, followed by treatment with enzyme substrate
- Readout on any colorimetric (412 nm) plate reader

View Alternative Names

Citrate (Si)-synthase, CS

2 Images
ELISA - Citrate Synthase Activity Assay Kit (AB119692)
  • ELISA

Supplier Data

ELISA - Citrate Synthase Activity Assay Kit (AB119692)

Example activity measurements from titrated HepG2 cells.

ELISA - Citrate Synthase Activity Assay Kit (AB119692)
  • ELISA

Supplier Data

ELISA - Citrate Synthase Activity Assay Kit (AB119692)

Example activity measurements from titrated of Human heart homogenate.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Cow, Human

Assay type

Enzyme activity

Sensitivity

= 1.56 µg/mL

Range

1.56 - 100 µg/mL

Assay time

3h 15m

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Citrate Synthase Activity Assay Kit (ab119692) is used to determine mitochondrial citrate synthase (CS) activity in a sample. The enzyme is immunocaptured within the wells of a microplate, and activity is determined by recording color development of TNB, which is generated in the reaction of citrate synthesis. The reaction product, TNB, absorbs at OD = 412 nm.

This kit immunocaptures only native citrate synthase from the samples. Using the immunocapture method allows the measurement of citrate synthase activity with simple sample preparation and no need for mitochondrial isolation.

This kit can be used with whole cell extracts or tissue homogenates.

Citrate synthase assay protocol summary
- add samples to wells
- incubate for 3 hrs to capture enzyme
- wash with wash buffer
- add activity solution
- analyze with microplate reader for 5-30 min at 20s intervals

Related and recommended products
For a citrate synthase assay without the antibody pulldown step, see Citrate synthase assay kit ab239712.

Other Notes
Citrate synthase (CS, O75390) is the initial enzyme of the tricarboxylic acid (TCA) cycle. This enzyme is a 51.7 kDa enzyme that catalyzes the reaction of 2 carbon acetyl CoA with 4 carbon oxaloacetate to form the 6 carbon citrate (EC 2.3.3.1). This enzyme is an exclusive marker of the mitochondrial matrix. Citrate synthase is found in nearly all cells capable of oxidative metabolism.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.45" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119692-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"10X Blocking Buffer", "number":"AB119692-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"20X Buffer", "number":"AB119692-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"10X Reaction Reagent", "number":"AB119692-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CS Microplate", "number":"AB119692-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"Extraction Buffer (ab260490)", "number":"AB119692-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"1X Base Buffer", "number":"AB119692-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Citrate synthetase also known as citrate synthase is an important enzyme in the tricarboxylic acid cycle. It catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and CoA. The enzyme weighs around 49 kilodaltons. It is expressed prominently in the mitochondria of eukaryotic cells where it initiates the Krebs cycle by providing citrate to be further processed. Citrate synthetase is an important point of control within this metabolic cycle.
Biological function summary

Citrate synthetase plays a central role in energy production by converting oxaloacetate and acetyl-CoA into citrate. This enzyme is not known to be part of any larger complex but associates with downstream enzymes such as aconitase in the metabolic pathway. Its activity is essential for cellular respiration impacting the overall metabolic rate of the organism. The concentration of citrate produced serves as a checkpoint both for the continuation of the Krebs cycle and for feedback inhibition of glycolysis.

Pathways

Citrate synthetase is integral to the Krebs cycle and the related oxidative phosphorylation pathway. These pathways are essential for efficient ATP production. Citrate synthetase works closely with enzymes like isocitrate dehydrogenase and alpha-ketoglutarate dehydrogenase within the Krebs cycle. Through these interactions citrate synthetase ensures the proper flow of carbon through the cycle impacting ATP yield and cellular energy homeostasis.

Citrate synthetase's function can influence metabolic diseases like diabetes and mitochondrial disorders. Alterations in its activity may contribute to the dysregulation of glucose metabolism seen in diabetes affecting enzymes like glucose transporter 4 (GLUT4). In mitochondrial disorders changes in its normal activity can cause energy production deficiencies influencing proteins such as cytochrome c which is critical in the electron transport chain. Understanding these interactions can help develop therapeutic strategies targeting metabolic pathways.
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Product protocols

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Target data

Key enzyme of the Krebs tricarboxylic acid cycle which catalyzes the synthesis of citrate from acetyl coenzyme A and oxaloacetate.
See full target information Citrate synthase, mitochondrial
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Publications (35)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 116:152-163 PubMed39479926

2024

Noncanonical TCA cycle fosters canonical TCA cycle and mitochondrial integrity in acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Atsushi Watanabe,Chartsiam Tipgomut,Haruhito Totani,Kentaro Yoshimura,Tomohiko Iwano,Hamed Bashiri,Lee Hui Chua,Chong Yang,Toshio Suda

Frontiers in molecular biosciences 11:1460987 PubMed39297074

2024

Recent progress of methods for cuproptosis detection.

Applications

Unspecified application

Species

Unspecified reactive species

Ligang Zhang,Ruiting Deng,Raoqing Guo,Yawen Jiang,Yichen Guan,Caiyue Chen,Wudi Zhao,Guobin Huang,Lian Liu,Hongli Du,Dongsheng Tang

Pflugers Archiv : European journal of physiology 476:939-948 PubMed38446167

2024

Locally applied heat stress during exercise training may promote adaptations to mitochondrial enzyme activities in skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Ed Maunder,Andrew King,Jeffrey A Rothschild,Matthew J Brick,Warren B Leigh,Christopher P Hedges,Troy L Merry,Andrew E Kilding

Journal of dairy science 107:1197-1210 PubMed37709028

2023

Peripheral blood mononuclear cell mitochondrial enzyme activity in calves is associated with average daily gain, reproductive outcomes, lactation performance, and survival.

Applications

Unspecified application

Species

Unspecified reactive species

A M Niesen,H A Rossow

Disease models & mechanisms 16: PubMed36695500

2023

Increased cysteine metabolism in PINK1 models of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Travaglio,Filippos Michopoulos,Yizhou Yu,Rebeka Popovic,Edmund Foster,Muireann Coen,L Miguel Martins

Frontiers in endocrinology 13:895593 PubMed35957832

2022

plays an important role in the pathogenesis of metabolic-associated fatty liver disease by regulating hepatic lipid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Yongqiang Ma,Guangshun Chen,Junfang Yi,Qiang Li,Zhi Tan,Wenling Fan,Xiaohua Luo,Zhiyong He,Zhongzhou Si,Jiequn Li

Journal of dairy science 105:7036-7046 PubMed35787326

2022

Peripheral blood mononuclear cell mitochondrial enzyme activity is associated with parity and lactation performance in early lactation Holstein dairy cows.

Applications

Unspecified application

Species

Unspecified reactive species

A M Niesen,O N Genther-Schroeder,C M K Bradley,J A Davidson,H A Rossow

ACS bio & med chem Au 2:282-296 PubMed35874496

2022

Discovery of JND003 as a New Selective Estrogen-Related Receptor α Agonist Alleviating Nonalcoholic Fatty Liver Disease and Insulin Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Liufeng Mao,Lijie Peng,Xiaomei Ren,Yi Chu,Tao Nie,Wanhua Lin,Xuemei Zhao,Andrew Libby,Yong Xu,Yu Chang,Chong Lei,Kerry Loomes,Na Wang,Jinsong Liu,Moshe Levi,Donghai Wu,Xiaoyan Hui,Ke Ding

Oxidative medicine and cellular longevity 2021:8240015 PubMed34777696

2021

Silencing LINC00294 Restores Mitochondrial Function and Inhibits Apoptosis of Glioma Cells under Hypoxia via the miR-21-5p/CASKIN1/cAMP Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaogang Dong,Qin Pi,Anwaier Yuemaierabola,Wenjia Guo,Hailong Tian

European journal of applied physiology 122:93-102 PubMed34562114

2021

Peak fat oxidation is positively associated with vastus lateralis CD36 content, fed-state exercise fat oxidation, and endurance performance in trained males.

Applications

Unspecified application

Species

Unspecified reactive species

Ed Maunder,Daniel J Plews,Gareth A Wallis,Matthew J Brick,Warren B Leigh,Wee-Leong Chang,Tom Stewart,Casey M Watkins,Andrew E Kilding
View all publications
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