Citrate Synthase Assay Kit (ab239712) provides reagents for the measurement of citrate synthase activity in various tissues/cells.
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Key enzyme of the Krebs tricarboxylic acid cycle which catalyzes the synthesis of citrate from acetyl coenzyme A and oxaloacetate.
Citrate (Si)-synthase, CS
Citrate Synthase Assay Kit (ab239712) provides reagents for the measurement of citrate synthase activity in various tissues/cells.
Citrate Synthase Assay Kit (ab239712) provides reagents for the measurement of citrate synthase activity in various tissues/cells. Citrate Synthase reacts with substrate mix to form an intermediate, which subsequently reacts with developer to generate the colored product. The rate of color development is proportional to the enzyme activity. The assay is simple, rapid and can detect Citrate Synthase activity less than 1 mU in a variety of samples.
This product is manufactured by BioVision, an Abcam company and was previously called K318 Citrate Synthase Activity Colorimetric Assay Kit. K318-100 is the same size as the 100 test size of ab239712.
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Citrate synthetase also known as citrate synthase is an important enzyme in the tricarboxylic acid cycle. It catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and CoA. The enzyme weighs around 49 kilodaltons. It is expressed prominently in the mitochondria of eukaryotic cells where it initiates the Krebs cycle by providing citrate to be further processed. Citrate synthetase is an important point of control within this metabolic cycle.
Citrate synthetase plays a central role in energy production by converting oxaloacetate and acetyl-CoA into citrate. This enzyme is not known to be part of any larger complex but associates with downstream enzymes such as aconitase in the metabolic pathway. Its activity is essential for cellular respiration impacting the overall metabolic rate of the organism. The concentration of citrate produced serves as a checkpoint both for the continuation of the Krebs cycle and for feedback inhibition of glycolysis.
Citrate synthetase is integral to the Krebs cycle and the related oxidative phosphorylation pathway. These pathways are essential for efficient ATP production. Citrate synthetase works closely with enzymes like isocitrate dehydrogenase and alpha-ketoglutarate dehydrogenase within the Krebs cycle. Through these interactions citrate synthetase ensures the proper flow of carbon through the cycle impacting ATP yield and cellular energy homeostasis.
Citrate synthetase's function can influence metabolic diseases like diabetes and mitochondrial disorders. Alterations in its activity may contribute to the dysregulation of glucose metabolism seen in diabetes affecting enzymes like glucose transporter 4 (GLUT4). In mitochondrial disorders changes in its normal activity can cause energy production deficiencies influencing proteins such as cytochrome c which is critical in the electron transport chain. Understanding these interactions can help develop therapeutic strategies targeting metabolic pathways.
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