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AB239712

Citrate Synthase Assay Kit

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(15 Publications)

Citrate Synthase Assay Kit (ab239712) is used to quantify citrate synthase activity.

- includes citrate synthase enzyme positive control
- Readout on any colorimetric (412 nm) plate reader

View Alternative Names

Citrate (Si)-synthase, CS

2 Images
Functional Studies - Citrate Synthase Assay Kit (AB239712)
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Functional Studies - Citrate Synthase Assay Kit (AB239712)

Citrate Synthase Activity in Jurkat cell lysate (10 µg), rat liver lysate (20 µg), purified yeast mitochondria (4 µg) and CS Positive Control (2 µL)

Functional Studies - Citrate Synthase Assay Kit (AB239712)
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Supplier Data

Functional Studies - Citrate Synthase Assay Kit (AB239712)

GSH Standard Curve

Key facts

Detection method

Colorimetric

Sample types

Tissue, Cell Lysate

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Citrate Synthase Assay Kit (ab239712) provides reagents for the measurement of citrate synthase activity in various tissues/cells. The assay can detect Citrate Synthase activity less than 1 mU in a variety of samples.

Citrate synthase assay principle
In the citrate synthase assay, citrate synthase acts on acetyl-CoA and oxaloacetate to produce citrate + CoA-SH. CoA-SH then reacts with DTNB to produce a colorimetric (412 nm) readout.

Citrate synthase assay protocol summary
- add samples and standards to wells
- add reaction mix
- Analyse on a colorimetric plate reader immediately in kinetic mode at room temperature for 20-40 mins.

Related and recommended products
This kit uses the same method as Citrate synthase activity assay kit ab119692, except without the antibody pull-down step used in ab119692 to isolate citrate synthase.

Other Notes
This product was previously called K318 Biovision Citrate Synthase Activity Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab239712-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"GSH Standard (Reduced)", "number":"AB239712-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"DTNB", "number":"AB239712-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"CS Substrate Mix", "number":"AB239712-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"CS Positive Control", "number":"AB239712-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 7", "number":"AB239712-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Citrate synthetase also known as citrate synthase is an important enzyme in the tricarboxylic acid cycle. It catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and CoA. The enzyme weighs around 49 kilodaltons. It is expressed prominently in the mitochondria of eukaryotic cells where it initiates the Krebs cycle by providing citrate to be further processed. Citrate synthetase is an important point of control within this metabolic cycle.
Biological function summary

Citrate synthetase plays a central role in energy production by converting oxaloacetate and acetyl-CoA into citrate. This enzyme is not known to be part of any larger complex but associates with downstream enzymes such as aconitase in the metabolic pathway. Its activity is essential for cellular respiration impacting the overall metabolic rate of the organism. The concentration of citrate produced serves as a checkpoint both for the continuation of the Krebs cycle and for feedback inhibition of glycolysis.

Pathways

Citrate synthetase is integral to the Krebs cycle and the related oxidative phosphorylation pathway. These pathways are essential for efficient ATP production. Citrate synthetase works closely with enzymes like isocitrate dehydrogenase and alpha-ketoglutarate dehydrogenase within the Krebs cycle. Through these interactions citrate synthetase ensures the proper flow of carbon through the cycle impacting ATP yield and cellular energy homeostasis.

Citrate synthetase's function can influence metabolic diseases like diabetes and mitochondrial disorders. Alterations in its activity may contribute to the dysregulation of glucose metabolism seen in diabetes affecting enzymes like glucose transporter 4 (GLUT4). In mitochondrial disorders changes in its normal activity can cause energy production deficiencies influencing proteins such as cytochrome c which is critical in the electron transport chain. Understanding these interactions can help develop therapeutic strategies targeting metabolic pathways.
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Product protocols

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Target data

Key enzyme of the Krebs tricarboxylic acid cycle which catalyzes the synthesis of citrate from acetyl coenzyme A and oxaloacetate.
See full target information Citrate synthase, mitochondrial
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40331969

2025

Carvacrol Protects IPEC-J2 Cells from Oxidative Stress by Suppressing Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Chun Hsu,Han-Tsung Wang,Ching-Yi Chen

International journal of molecular sciences 26: PubMed40076489

2025

Integrin-Linked Kinase (ILK) Promotes Mitochondrial Dysfunction by Decreasing CPT1A Expression in a Folic Acid-Based Model of Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Mariano de la Serna-Soto,Laura Calleros,María Martos-Elvira,Ariadna Moreno-Piedra,Sergio García-Villoria,Mercedes Griera,Elena Alcalde-Estévez,Ana Asenjo-Bueno,Diego Rodríguez-Puyol,Sergio de Frutos,María Piedad Ruiz-Torres

Molecular medicine (Cambridge, Mass.) 31:54 PubMed39930360

2025

Licochalcone A prevents cognitive decline in a lipopolysaccharide-induced neuroinflammation mice model.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Carrasco,Laura Guzman,Jordi Olloquequi,Amanda Cano,Ana Fortuna,Manuel Vazquez-Carrera,Ester Verdaguer,Carme Auladell,Miren Ettcheto,Antoni Camins

Human molecular genetics 33:1195-1206 PubMed38621658

2024

Mitochondrial abnormalities contribute to muscle weakness in a Dnajb6 deficient zebrafish model.

Applications

Unspecified application

Species

Unspecified reactive species

Emily A McKaige,Clara Lee,Vanessa Calcinotto,Saveen Giri,Simon Crawford,Meagan J McGrath,Georg Ramm,Robert J Bryson-Richardson

Journal of diabetes research 2024:5549762 PubMed38435452

2024

The Effects of Resistance Exercise Training on Skeletal Muscle Metabolism and Insulin Resistance Development in Female Rodents with Type 1 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Mitchell J Sammut,David P McBey,Amit P Sayal,C W James Melling

Scientific reports 14:553 PubMed38177205

2024

The mitochondrial DNA common deletion as a potential biomarker of cancer-associated fibroblasts from skin basal and squamous cell carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriele A Fontana,Michael R MacArthur,Nadezhda Rotankova,Michela Di Filippo,Hans-Dietmar Beer,Hailey L Gahlon

Frontiers in physiology 14:1263500 PubMed37942230

2023

Treadmill training does not enhance skeletal muscle recovery following disuse atrophy in older male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Elena M Yee,Carson T Hauser,Jonathan J Petrocelli,Naomi M M P de Hart,Patrick J Ferrara,Princess Bombyck,Zachary J Fennel,Lisha van Onselen,Sohom Mookerjee,Katsuhiko Funai,J David Symons,Micah J Drummond

Molecular metabolism 78:101814 PubMed37802398

2023

ERRα fosters running endurance by driving myofiber aerobic transformation and fuel efficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Xia,Charlotte Scholtes,Catherine R Dufour,Christina Guluzian,Vincent Giguère

Molecular metabolism 77:101802 PubMed37690520

2023

Partial skeletal muscle-specific Drp1 knockout enhances insulin sensitivity in diet-induced obese mice, but not in lean mice.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin A Kugler,Jared Lourie,Nicolas Berger,Nana Lin,Paul Nguyen,Edzana DosSantos,Abir Ali,Amira Sesay,H Grace Rosen,Baby Kalemba,Gregory M Hendricks,Joseph A Houmard,Hiromi Sesaki,Philimon Gona,Tongjian You,Zhen Yan,Kai Zou

Global change biology 29:6969-6987 PubMed37464471

2023

Triploid Pacific oysters exhibit stress response dysregulation and elevated mortality following heatwaves.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew N George,Olivia Cattau,Mollie A Middleton,Delaney Lawson,Brent Vadopalas,Mackenzie Gavery,Steven B Roberts
View all publications
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