Citrate Synthase Assay Kit (ab239712) is used to quantify citrate synthase activity.
- includes citrate synthase enzyme positive control
- Readout on any colorimetric (412 nm) plate reader
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- Scientific reports 14:5532024The mitochondrial DNA common deletion as a potential biomarker of cancer-associated fibroblasts from skin basal and squamous cell carcinomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGabriele A Fontana,Michael R MacArthur,Nadezhda Rotankova,Michela Di Filippo,Hans-Dietmar Beer,Hailey L GahlonPubMed 38177205
- Frontiers in physiology 14:12635002023Treadmill training does not enhance skeletal muscle recovery following disuse atrophy in older male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElena M Yee,Carson T Hauser,Jonathan J Petrocelli,Naomi M M P de Hart,Patrick J Ferrara,Princess Bombyck,Zachary J Fennel,Lisha van Onselen,Sohom Mookerjee,Katsuhiko Funai,J David Symons,Micah J DrummondPubMed 37942230
- Molecular metabolism 77:1018022023Partial skeletal muscle-specific Drp1 knockout enhances insulin sensitivity in diet-induced obese mice, but not in lean mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenjamin A Kugler,Jared Lourie,Nicolas Berger,Nana Lin,Paul Nguyen,Edzana DosSantos,Abir Ali,Amira Sesay,H Grace Rosen,Baby Kalemba,Gregory M Hendricks,Joseph A Houmard,Hiromi Sesaki,Philimon Gona,Tongjian You,Zhen Yan,Kai ZouPubMed 37690520
- Global change biology 29:6969-69872023Triploid Pacific oysters exhibit stress response dysregulation and elevated mortality following heatwaves.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew N George,Olivia Cattau,Mollie A Middleton,Delaney Lawson,Brent Vadopalas,Mackenzie Gavery,Steven B RobertsPubMed 37464471
- Antioxidants (Basel, Switzerland) 12:2023Intraoperative Hemi-Diaphragm Electrical Stimulation Demonstrates Attenuated Mitochondrial Function without Change in Oxidative Stress in Cardiothoracic Surgery Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobert T Mankowski,Stephanie E Wohlgemuth,Guilherme Bresciani,A Daniel Martin,George Arnaoutakis,Tomas Martin,Eric Jeng,Leonardo Ferreira,Tiago Machuca,Mindaugas Rackauskas,Ashley J Smuder,Thomas Beaver,Christiaan Leeuwenburgh,Barbara K SmithPubMed 37237876
- Nature communications 13:49182022Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Xue Xie,Nima N Naseri,Jasmine Fels,Parinati Kharel,Yoonmi Na,Diane Lane,Jacqueline Burré,Manu SharmaPubMed 35995799
- PloS one 16:e02588312021Effect of immune modulation on the skeletal muscle mitochondrial exercise response: An exploratory study in mice with cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinda A Buss,Barry Hock,Troy L Merry,Abel D Ang,Bridget A Robinson,Margaret J Currie,Gabi U DachsPubMed 34665826
- Nature communications 12:1022021Improvement of obesity-associated disorders by a small-molecule drug targeting mitochondria of adipose tissue macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYawei Wang et. al.PubMed 33397994
- British journal of pharmacology 176:3983-40012019Myricanol modulates skeletal muscle-adipose tissue crosstalk to alleviate high-fat diet-induced obesity and insulin resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengnan Shen et. al.PubMed 31339170
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Cell Lysate
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Target data
Function
Key enzyme of the Krebs tricarboxylic acid cycle which catalyzes the synthesis of citrate from acetyl coenzyme A and oxaloacetate.
Alternative names
Citrate (Si)-synthase, CS
What's included?
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Citrate Synthase Assay Kit (ab239712) is used to quantify citrate synthase activity.
- includes citrate synthase enzyme positive control
- Readout on any colorimetric (412 nm) plate reader
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Cell Lysate
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Citrate Synthase Assay Kit (ab239712) provides reagents for the measurement of citrate synthase activity in various tissues/cells. The assay can detect Citrate Synthase activity less than 1 mU in a variety of samples.
Citrate synthase assay principle
In the citrate synthase assay, citrate synthase acts on acetyl-CoA and oxaloacetate to produce citrate + CoA-SH. CoA-SH then reacts with DTNB to produce a colorimetric (412 nm) readout.
Citrate synthase assay protocol summary
- add samples and standards to wells
- add reaction mix
- Analyse on a colorimetric plate reader immediately in kinetic mode at room temperature for 20-40 mins.
Related and recommended products
This kit uses the same method as Citrate synthase activity assay kit Citrate Synthase Activity Assay Kit ab119692, except without the antibody pull-down step used in Citrate Synthase Activity Assay Kit ab119692 to isolate citrate synthase.
Other Notes
This product was previously called K318 Biovision Citrate Synthase Activity Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Citrate synthetase also known as citrate synthase is an important enzyme in the tricarboxylic acid cycle. It catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and CoA. The enzyme weighs around 49 kilodaltons. It is expressed prominently in the mitochondria of eukaryotic cells where it initiates the Krebs cycle by providing citrate to be further processed. Citrate synthetase is an important point of control within this metabolic cycle.
- Biological function summary
Citrate synthetase plays a central role in energy production by converting oxaloacetate and acetyl-CoA into citrate. This enzyme is not known to be part of any larger complex but associates with downstream enzymes such as aconitase in the metabolic pathway. Its activity is essential for cellular respiration impacting the overall metabolic rate of the organism. The concentration of citrate produced serves as a checkpoint both for the continuation of the Krebs cycle and for feedback inhibition of glycolysis.
- Pathways
Citrate synthetase is integral to the Krebs cycle and the related oxidative phosphorylation pathway. These pathways are essential for efficient ATP production. Citrate synthetase works closely with enzymes like isocitrate dehydrogenase and alpha-ketoglutarate dehydrogenase within the Krebs cycle. Through these interactions citrate synthetase ensures the proper flow of carbon through the cycle impacting ATP yield and cellular energy homeostasis.
- Associated diseases and disorders
Citrate synthetase's function can influence metabolic diseases like diabetes and mitochondrial disorders. Alterations in its activity may contribute to the dysregulation of glucose metabolism seen in diabetes affecting enzymes like glucose transporter 4 (GLUT4). In mitochondrial disorders changes in its normal activity can cause energy production deficiencies influencing proteins such as cytochrome c which is critical in the electron transport chain. Understanding these interactions can help develop therapeutic strategies targeting metabolic pathways.
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Functional Studies - Citrate Synthase Assay Kit (ab239712)
Functional Studies - Citrate Synthase Assay Kit (ab239712)
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