Cleaved Caspase-3 Staining Kit (FITC) ab65613 provides a convenient means for sensitive detection of activated Caspase-3 in living cells.
Images
Publications
Filter by
- Molecular cancer therapeutics 20:1680-16912021PARP and CDK4/6 Inhibitor Combination Therapy Induces Apoptosis and Suppresses Neuroendocrine Differentiation in Prostate Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Wu,Shan Peng,Patrick G Pilié,Chuandong Geng,Sanghee Park,Ganiraju C Manyam,Yungang Lu,Guang Yang,Zhe Tang,Shakuntala Kondraganti,Daoqi Wang,Courtney W Hudgens,Debora A Ledesma,Mario L Marques-Piubelli,Carlos A Torres-Cabala,Jonathan L Curry,Patricia Troncoso,Paul G Corn,Bradley M Broom,Timothy C ThompsonPubMed 34158347
- iScience 24:1026642021CHK1 inhibitor sensitizes resistant colorectal cancer stem cells to nortopsentin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimone Di Franco,Barbara Parrino,Miriam Gaggianesi,Vincenzo Davide Pantina,Paola Bianca,Annalisa Nicotra,Laura Rosa Mangiapane,Melania Lo Iacono,Gloria Ganduscio,Veronica Veschi,Ornella Roberta Brancato,Antonino Glaviano,Alice Turdo,Irene Pillitteri,Lorenzo Colarossi,Stella Cascioferro,Daniela Carbone,Camilla Pecoraro,Micol Eleonora Fiori,Ruggero De Maria,Matilde Todaro,Isabella Screpanti,Girolamo Cirrincione,Patrizia Diana,Giorgio StassiPubMed 34169240
- Cancer 127:1606-16192021Angiotensin II receptor blockers valsartan and losartan improve survival rate clinically and suppress tumor growth via apoptosis related to PI3K/AKT signaling in nasopharyngeal carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Tsai Lin,Hung-Chen Wang,Ming-Hsien Tsai,Yan-Ye Su,Ming-Yu Yang,Chih-Yen ChienPubMed 33405241
- The Journal of experimental medicine 217:2020Noncanonical STAT3 activity sustains pathogenic Th17 proliferation and cytokine response to antigen.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCatherine H Poholek,Itay Raphael,Dongwen Wu,Shankar Revu,Natalie Rittenhouse,Uzodinma U Uche,Saikat Majumder,Lawrence P Kane,Amanda C Poholek,Mandy J McGeachyPubMed 32697822
- Radiation oncology (London, England) 15:1312020Novel role of PAF1 in attenuating radiosensitivity in cervical cancer by inhibiting IER5 transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing-Jie Zheng,Yue He,Yang Liu,Feng-Shuang Li,Zhen Cui,Xiao-Meng Du,Chun-Peng Wang,Yu-Mei WuPubMed 32471508
- Neurotoxicity research 37:743-7522020Some Ototoxic Drugs Destroy Cochlear Support Cells Before Damaging Sensory Hair Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDalian Ding et. al.PubMed 31997155
- EBioMedicine 47:114-1272019Augmenting the therapeutic efficacy of adenosine against pancreatic cancer by switching the Akt/p21-dependent senescence to apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongqin Yang,Qi Zhang,Yunfang Ma,Zhihui Che,Wenli Zhang,Mengmeng Wu,Lijun Wu,Fuchen Liu,Yiwei Chu,Wei Xu,Mary McGrath,Chunhua Song,Jie LiuPubMed 31495718
- Clinical cancer research : an official journal of 25:5548-55602019Effective Delivery of a Microtubule Polymerization Inhibitor Synergizes with Standard Regimens in Models of Pancreatic Ductal Adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJaime A Eberle-Singh et. al.PubMed 31175095
- International journal of nanomedicine 13:3235-32502018Chitosan gold nanoparticles induce cell death in HeLa and MCF-7 cells through reactive oxygen species production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Carolina Martínez-Torres et. al.PubMed 29910612
- Hearing research 365:110-1262018Differential fates of tissue macrophages in the cochlea during postnatal development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYouyi Dong,Celia Zhang,Mitchell Frye,Weiping Yang,Dalian Ding,Ashu Sharma,Weiwei Guo,Bo Hua HuPubMed 29804721
Key facts
- Sample types
Suspension cells, Adherent cells
- Assay type
Enzyme activity
- Assay time
2h
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Involved in the activation cascade of caspases responsible for apoptosis execution (PubMed:7596430). At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond (PubMed:7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (PubMed:7596430). Involved in the cleavage of huntingtin (PubMed:8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800).
Alternative names
Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1, CASP3, CPP32
What's included?
Recommended products
Cleaved Caspase-3 Staining Kit (FITC) ab65613 provides a convenient means for sensitive detection of activated Caspase-3 in living cells.
Alternative names
Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1, CASP3, CPP32
Key facts
- Sample types
Suspension cells, Adherent cells
- Assay type
Enzyme activity
- Assay time
2h
- Assay Platform
Microplate reader, Fluor. microscope, Flow cyt.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Cleaved Caspase-3 Staining Kit (FITC) ab65613 provides a convenient means for sensitive detection of activated Caspase-3 in living cells.
The cleaved caspase 3 assay utilizes the Caspase-3 inhibitor, DEVD-FMK, conjugated to FITC (FITC-DEVD-FMK) as a marker. FITC-DEVD-FMK is cell permeable, non-toxic, and irreversibly binds to activated Caspase-3 in apoptotic cells. The FITC label allows detection of activated / cleaved caspase-3 in apoptotic cells directly by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
Previously called Caspase-3 (active) FITC Staining Kit **Other caspase and apoptosis assays** Review the full set of , or the .
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Mart?nez-Torres, Ana Carolina et al., BMC cancer?vol. 18,1 13., Fig 6, doi:10.1186/s12885-017-3954-5Functional Studies - Cleaved Caspase-3 Staining Kit (FITC) (ab65613)
Caspase-3 activation was measured by flow cytometry, using ab65613, in cells that were left alone or pretreated with QVD.oph and then treated with I-CRP (1.25 U/ml) or a drug that induces DNA strand breaks (100 μM) for 24 h, data was then analyzed and graphed.
Functional Studies - Cleaved Caspase-3 Staining Kit (FITC) (ab65613)
Caspase-3 in Jurkat cells following 24 hour exposure to 2 uM Camptothecin (CPT; Camptothecin, DNA topoisomerase inhibitor ab120115) with or without 20 nM caspase inhibitor Z-VAD(OMe)-FMK (zVAD; Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor ab120487). Background signal subtracted, duplicates; +/- SD.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com