Cleaved Caspase-3 Staining Kit (FITC) ab65613 provides a convenient means for sensitive detection of activated Caspase-3 in living cells.
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- Molecular cancer therapeutics 20:1680-16912021PARP and CDK4/6 Inhibitor Combination Therapy Induces Apoptosis and Suppresses Neuroendocrine Differentiation in Prostate Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Wu,Shan Peng,Patrick G Pilié,Chuandong Geng,Sanghee Park,Ganiraju C Manyam,Yungang Lu,Guang Yang,Zhe Tang,Shakuntala Kondraganti,Daoqi Wang,Courtney W Hudgens,Debora A Ledesma,Mario L Marques-Piubelli,Carlos A Torres-Cabala,Jonathan L Curry,Patricia Troncoso,Paul G Corn,Bradley M Broom,Timothy C ThompsonPubMed 34158347
- iScience 24:1026642021CHK1 inhibitor sensitizes resistant colorectal cancer stem cells to nortopsentin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimone Di Franco,Barbara Parrino,Miriam Gaggianesi,Vincenzo Davide Pantina,Paola Bianca,Annalisa Nicotra,Laura Rosa Mangiapane,Melania Lo Iacono,Gloria Ganduscio,Veronica Veschi,Ornella Roberta Brancato,Antonino Glaviano,Alice Turdo,Irene Pillitteri,Lorenzo Colarossi,Stella Cascioferro,Daniela Carbone,Camilla Pecoraro,Micol Eleonora Fiori,Ruggero De Maria,Matilde Todaro,Isabella Screpanti,Girolamo Cirrincione,Patrizia Diana,Giorgio StassiPubMed 34169240
- Cancer 127:1606-16192021Angiotensin II receptor blockers valsartan and losartan improve survival rate clinically and suppress tumor growth via apoptosis related to PI3K/AKT signaling in nasopharyngeal carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Tsai Lin,Hung-Chen Wang,Ming-Hsien Tsai,Yan-Ye Su,Ming-Yu Yang,Chih-Yen ChienPubMed 33405241
- The Journal of experimental medicine 217:2020Noncanonical STAT3 activity sustains pathogenic Th17 proliferation and cytokine response to antigen.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCatherine H Poholek,Itay Raphael,Dongwen Wu,Shankar Revu,Natalie Rittenhouse,Uzodinma U Uche,Saikat Majumder,Lawrence P Kane,Amanda C Poholek,Mandy J McGeachyPubMed 32697822
- Radiation oncology (London, England) 15:1312020Novel role of PAF1 in attenuating radiosensitivity in cervical cancer by inhibiting IER5 transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing-Jie Zheng,Yue He,Yang Liu,Feng-Shuang Li,Zhen Cui,Xiao-Meng Du,Chun-Peng Wang,Yu-Mei WuPubMed 32471508
- Neurotoxicity research 37:743-7522020Some Ototoxic Drugs Destroy Cochlear Support Cells Before Damaging Sensory Hair Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDalian Ding et. al.PubMed 31997155
- EBioMedicine 47:114-1272019Augmenting the therapeutic efficacy of adenosine against pancreatic cancer by switching the Akt/p21-dependent senescence to apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongqin Yang,Qi Zhang,Yunfang Ma,Zhihui Che,Wenli Zhang,Mengmeng Wu,Lijun Wu,Fuchen Liu,Yiwei Chu,Wei Xu,Mary McGrath,Chunhua Song,Jie LiuPubMed 31495718
- Clinical cancer research : an official journal of 25:5548-55602019Effective Delivery of a Microtubule Polymerization Inhibitor Synergizes with Standard Regimens in Models of Pancreatic Ductal Adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJaime A Eberle-Singh et. al.PubMed 31175095
- International journal of nanomedicine 13:3235-32502018Chitosan gold nanoparticles induce cell death in HeLa and MCF-7 cells through reactive oxygen species production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Carolina Martínez-Torres et. al.PubMed 29910612
- Hearing research 365:110-1262018Differential fates of tissue macrophages in the cochlea during postnatal development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYouyi Dong,Celia Zhang,Mitchell Frye,Weiping Yang,Dalian Ding,Ashu Sharma,Weiwei Guo,Bo Hua HuPubMed 29804721
Key facts
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Enzyme activity
- Assay time
- 2h
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Target data
Function
Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis (PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:35338844, PubMed:35446120, PubMed:7596430). Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins (PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:7596430). At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond (PubMed:10497198, PubMed:16374543, PubMed:7596430, PubMed:7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity). Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively) (PubMed:7596430). Cleaves and inactivates interleukin-18 (IL18) (PubMed:37993714, PubMed:9334240). Involved in the cleavage of huntingtin (PubMed:8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800). Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed:30878284). Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) (PubMed:35338844, PubMed:35446120). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed:23845944, PubMed:33725486). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed:36758104, PubMed:36758106).
Alternative names
CPP32, CASP3, Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1
What's included?
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Cleaved Caspase-3 Staining Kit (FITC) ab65613 provides a convenient means for sensitive detection of activated Caspase-3 in living cells.
Key facts
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Enzyme activity
- Assay time
- 2h
- Assay Platform
- Microplate reader, Fluor. microscope, Flow cyt.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Cleaved Caspase-3 Staining Kit (FITC) ab65613 provides a convenient means for sensitive detection of activated Caspase-3 in living cells.
The cleaved caspase 3 assay utilizes the Caspase-3 inhibitor, DEVD-FMK, conjugated to FITC (FITC-DEVD-FMK) as a marker. FITC-DEVD-FMK is cell permeable, non-toxic, and irreversibly binds to activated Caspase-3 in apoptotic cells. The FITC label allows detection of activated / cleaved caspase-3 in apoptotic cells directly by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
Other Notes
Previously called Caspase-3 (active) FITC Staining Kit
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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2 product images
Martinez-Torres, Ana Carolina et al., BMC cancer-vol. 18,1 13., Fig 6, doi:10.1186/s12885-017-3954-5Functional Studies - Cleaved Caspase-3 Staining Kit (FITC) (ab65613)
Caspase-3 activation was measured by flow cytometry, using ab65613, in cells that were left alone or pretreated with QVD.oph and then treated with I-CRP (1.25 U/ml) or a drug that induces DNA strand breaks (100 μM) for 24 h, data was then analyzed and graphed.
Functional Studies - Cleaved Caspase-3 Staining Kit (FITC) (ab65613)
Caspase-3 in Jurkat cells following 24 hour exposure to 2 uM Camptothecin (CPT; Camptothecin, DNA topoisomerase inhibitor ab120115) with or without 20 nM caspase inhibitor Z-VAD(OMe)-FMK (zVAD; Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor ab120487). Background signal subtracted, duplicates; +/- SD.
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