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Cleaved Caspase-3 Staining Kit (Red) ab65617 provides a convenient means for detecting activated Caspase-3 in living cells.

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Images

Functional Studies - Cleaved Caspase-3 Staining Kit (Red) (AB65617), expandable thumbnail

Publications

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Enzyme activity

Assay time

2h

Associated Products

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Target data

Function

Involved in the activation cascade of caspases responsible for apoptosis execution (PubMed:7596430). At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond (PubMed:7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (PubMed:7596430). Involved in the cleavage of huntingtin (PubMed:8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800).

Alternative names

Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1, CASP3, CPP32

What's included?

100 Test
Components
Red-DEVD-FMK
1 x 100 µL
Wash Buffer IV
2 x 100 mL
Z-VAD-FMK
1 x 10 µL

Recommended products

Cleaved Caspase-3 Staining Kit (Red) ab65617 provides a convenient means for detecting activated Caspase-3 in living cells.

Alternative names

Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1, CASP3, CPP32

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Enzyme activity

Assay time

2h

Assay Platform

Microplate reader, Fluor. microscope, Flow cyt.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Cleaved Caspase-3 Staining Kit (Red) ab65617 provides a convenient means for detecting activated Caspase-3 in living cells. The assay utilizes the Caspase-3 inhibitor DEVD-FMK conjugated to sulfo-rhodamine (Red-DEVD-FMK) as the fluorescent in situ marker. Red-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells.

This product is manufactured by BioVision, an Abcam company and was previously called K193 CaspGLOW™ Red Active Caspase-3 Staining Kit. K193-100 is the same size as the 100 test size of ab65617.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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