Coenzyme A Assay Kit

Product details

Coenzyme A Assay Kit ab102504 provides an easy, accurate assay to measure the CoA level in biological samples. The assay can detect 0.1 to 10 nmol of CoA (2.5-250 uM concentration range) in a variety of samples.

Coenzyme A assay principle
In the Coenzyme A assay, Acyl-CoA synthetase acts on Coenzyme A to produce Acyl-CoA. The action of Acyl-CoA oxidase on Acyl-CoA then produces hydrogen peroxide, which reacts with the OxiRed Probe. This produces a colored (570 nm) and fluorescent (Ex/Em 535/587 nm) readout.

Coenzyme A assay protocol summary
- add samples and standard to wells
- add ACS Substrate and CoA Converter Mix and incubate for 30-min at 37°C
- add reaction mix and incubate for 30-min at 37°C
- analyze with a plate reader

Other Notes
This product was previously called K367 Biovision Coenzyme A (CoA) Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of CoA is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-CoA. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.