Coenzyme A Assay Kit ab102504 provides an easy, accurate assay to measure the CoA level in variety biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Orphanet journal of rare diseases 15:2482020Pilot trial on the efficacy and safety of pantethine in children with pantothenate kinase-associated neurodegeneration: a single-arm, open-label study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuting Chang et. al.PubMed 32928263
- The Journal of biological chemistry 295:12485-124972020Tafazzin deficiency impairs CoA-dependent oxidative metabolism in cardiac mitochondria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCatherine H Le,Lindsay G Benage,Kalyn S Specht,Lance C Li Puma,Christopher M Mulligan,Adam L Heuberger,Jessica E Prenni,Steven M Claypool,Kathryn C Chatfield,Genevieve C Sparagna,Adam J ChiccoPubMed 32665401
- Nature communications 10:39292019Acetyl-CoA flux regulates the proteome and acetyl-proteome to maintain intracellular metabolic crosstalk.Applications:Unspecified applicationReactive species:Unspecified reactive speciesInca A Dieterich,Alexis J Lawton,Yajing Peng,Qing Yu,Timothy W Rhoads,Katherine A Overmyer,Yusi Cui,Eric A Armstrong,Porsha R Howell,Maggie S Burhans,Lingjun Li,John M Denu,Joshua J Coon,Rozalyn M Anderson,Luigi PuglielliPubMed 31477734
- American journal of physiology. Renal physiology :2019TGF-β1 modifies histone acetylation and acetyl-coenzyme A metabolism in renal myofibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEdward R Smith,Belinda Wigg,Stephen Holt,Timothy David HewitsonPubMed 30623724
- American journal of human genetics 102:1018-10302018Mutations in PPCS, Encoding Phosphopantothenoylcysteine Synthetase, Cause Autosomal-Recessive Dilated Cardiomyopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArcangela Iuso et. al.PubMed 29754768
- Digestive diseases and sciences 61:137-482015Vitamin B5 and N-Acetylcysteine in Nonalcoholic Steatohepatitis: A Preclinical Study in a Dietary Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMariana Verdelho Machado,Leandi Kruger,Mark L Jewell,Gregory Alexander Michelotti,Thiago de Almeida Pereira,Guanhua Xie,Cynthia A Moylan,Anna Mae DiehlPubMed 26403427
Key facts
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate
- Assay type
Quantitative
- Range
2.5 - 250 µM
- Assay time
1h
- Sensitivity
> 2.5 µM
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Coenzyme A Assay Kit ab102504 provides an easy, accurate assay to measure the CoA level in variety biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate
- Assay type
Quantitative
- Range
2.5 - 250 µM
- Assay time
1h
- Assay Platform
Microplate reader
- Sensitivity
> 2.5 µM
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Abcam's Coenzyme A Assay Kit provides an easy, accurate assay to measure the CoA level in variety biological samples. In the assay, free CoA is specifically utilized to generate products which react with OxiRed Probe to generate color (λ = 570 nm) and fluorescence (Ex=535/Em=587 nm). The assay can detect 0.1 to 10 nmol of CoA (2.5-250 μM concentration range) in a variety of samples.
Visit our FAQs page for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called K367 Coenzyme A (CoA) Colorimetric/Fluorometric Assay Kit. K367-100 is the same size as the 100 test size of ab102504.
Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of CoA is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-CoA. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Coenzyme A (CoA) is an important enzyme cofactor with a molecular mass of approximately 767.5 Da. Alternate names include CoA-SH and acetyl coenzyme A when bonded to an acetyl group. CoA is widely expressed in both prokaryotic and eukaryotic cells including the liver heart and brain tissues playing a significant role in metabolic processes. It functions mechanically by carrying acyl groups such as acetate which is vital for the conversion of pyruvate in the Krebs cycle and fatty acid synthesis.
- Biological function summary
This cofactor is important for various enzymatic reactions especially those involving the metabolism of carbohydrates fats and amino acids. It is not a part of a larger protein complex but works closely with enzymes like acetyl-CoA carboxylase. Its ability to form energy-rich thioester bonds allows for the transfer of acyl groups making it essential for energy production and biosynthetic reactions in cells. CoA transfers these acetyl groups in forms like acetyl coenzyme A or acetyl CoA which are critical for cellular functions.
- Pathways
CoA is essential in both the citric acid cycle (Krebs cycle) and fatty acid metabolism. In the citric acid cycle it helps convert pyruvate into acetyl-CoA therefore linking glycolysis to energy production. In fatty acid metabolism CoA participates in the process of beta-oxidation. Both pathways involve enzymes such as pyruvate dehydrogenase and fatty acid synthase which collaborate with CoA in important steps.
- Associated diseases and disorders
Perturbations in CoA metabolism are associated with conditions such as neurodegenerative diseases and metabolic disorders like diabetes. Alterations in CoA levels may affect pathways involving proteins such as pyruvate dehydrogenase and acetyl-CoA carboxylase which are important in maintaining cellular energy balance and lipid metabolism. These links highlight the importance of CoA in health and disease contexts.
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2 product images
Functional Studies - Coenzyme A Assay Kit (ab102504)
Example of Standard Curve obtained using ab102504.
Functional Studies - Coenzyme A Assay Kit (ab102504)
Example of Standard Curve obtained using ab102504.
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