Coenzyme A Assay Kit (Fluorometric - Green)
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Functional Studies - Coenzyme A Assay Kit (Fluorometric - Green) (AB138889)
Sample Standard Curve. CoA dose response was measured in a 96-well black plate with Coenzyme A Detection Kit using a microplate reader. As low as 40 nM (4 pmol/well) of CoA was detected with 30 minutes incubation time (n=3).
Product details
Coenzyme A Assay Kit (Fluorometric - Green) (ab138889) provides an ultrasensitive fluorometric assay to quantitate CoA content by detection of the -SH group in CoA.
The fluorogenic CoA green indicator dye used in the kit becomes strongly fluorescent upon reacting with -SH.
The assay kit can detect as little as 4 pmol CoA/ 100 uL assay volume (40 nM). It can be performed in a convenient 96-well or 384-well microtiter-plate format at Ex/Em = 490/520 nm, and easily adapted to automation without a separation step.
Coenzyme A assay protocol summary:
- add samples and standards to wells
- add reaction mix
- incubate for 10-60 min whilst measuring fluorescence with a microplate reader
The kit is for solution-based inhibitors or activators of Coenzyme A only. Any samples that contain proteins or thiol-containing compounds are not compatible with the kit.
Other Notes
Previously called Coenzyme A Detection kit (Fluorometric - Green).
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This cofactor is important for various enzymatic reactions especially those involving the metabolism of carbohydrates fats and amino acids. It is not a part of a larger protein complex but works closely with enzymes like acetyl-CoA carboxylase. Its ability to form energy-rich thioester bonds allows for the transfer of acyl groups making it essential for energy production and biosynthetic reactions in cells. CoA transfers these acetyl groups in forms like acetyl coenzyme A or acetyl CoA which are critical for cellular functions.
Pathways
CoA is essential in both the citric acid cycle (Krebs cycle) and fatty acid metabolism. In the citric acid cycle it helps convert pyruvate into acetyl-CoA therefore linking glycolysis to energy production. In fatty acid metabolism CoA participates in the process of beta-oxidation. Both pathways involve enzymes such as pyruvate dehydrogenase and fatty acid synthase which collaborate with CoA in important steps.
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Publications (6)
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International journal of molecular sciences 23: PubMed36361705
2022
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Communications biology 4:454 PubMed33846551
2021
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Blood 137:2090-2102 PubMed33529321
2021
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Biochemical and biophysical research communication 530:136-141 PubMed32828275
2020
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JIMD reports 44:1-7 PubMed29923093
2018
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American journal of human genetics 102:1018-1030 PubMed29754768
2018
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