Collagen Degradation/Zymography Assay Kit (Fluorometric)

Collagenase (Collagen Degradation/Zymography) Assay Kit (Fluorometric) (ab234624) employs a highly quenched collagen substrate which upon cleavage by a suitable collagenase releases a fluorophore, which can be easily quantified using a conventional microplate reader. This method is substrate-specific, simple, fast, high-throughput adaptable and amenable to the sensitive detection of collagenase activity (as low as 0.6 mCDU for bacterial collagenase) in biological samples.

This product is manufactured by BioVision, an Abcam company and was previously called K490 Collagenase (Collagen Degradation/Zymography) Assay Kit (Fluorometric). K490-100 is the same size as the 100 test size of ab234624.

Collagenases, members of the matrix metalloproteinase (MMP) family, are endopeptidases that digest native collagen in the triple helix region and are involved in the physiological and pathological turnover of connective tissues. Collagens are the major fibrous component of animal extracellular connective tissue. Bacterial collagenases differ from vertebrate collagenases in that they exhibit broader substrate specificity. Collagenases may play a role in facilitating tumor cell invasion of the extracellular matrix at multiple stages of the metastatic process. Collagenases have also been approved for medical uses for the treatment of Dupuytren's contracture, Peyronie's disease and wound healing.