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AB196999

Collagenase Activity Assay Kit (Colorimetric)

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(8 Publications)

Collagenase Activity Assay Kit ab196999 provides a quick and easy way to determine activity of Collagenase.

- Simple and fast protocol with results in less than one hour
- Suitable for measuring activity of bacterial collagenases such as from Clostridium histolyticum type I-XI
- Can be used for screening or characterization of collagenase inhibitors.
-Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

Collagenase ColH, Class II collagenase, Gelatinase ColH, Microbial collagenase, colH

3 Images
Functional Studies - Collagenase Activity Assay Kit (Colorimetric) (AB196999)
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Supplier Data

Functional Studies - Collagenase Activity Assay Kit (Colorimetric) (AB196999)

Collagenase activity over 10 minutes.

Functional Studies - Collagenase Activity Assay Kit (Colorimetric) (AB196999)
  • FuncS

Supplier Data

Functional Studies - Collagenase Activity Assay Kit (Colorimetric) (AB196999)

Enzyme activity of collagenase and inhibition by 10 mM (1,10)-Phenanthroline. Assay was performed following the kit protocol.

Other - Collagenase Activity Assay Kit (Colorimetric) (AB196999)
  • Other

Supplier Data

Other - Collagenase Activity Assay Kit (Colorimetric) (AB196999)

Representative image of Collagenase Activity Assay Kit (Colorimetric) ab196999

Components shown from left to right :

- Collagenase

- Inhibitor (1,10-Phenathroline)

- Collagenase Substrate I

- Collagenase Assay Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Purified protein, Cell Lysate

Assay type

Enzyme activity

Sensitivity

> 20 µU

Assay time

25m

Assay Platform

Microplate reader

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Secondary Antibodies

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

How the assay works

Collagenase Activity Assay Kit (Colorimetric) measures collagenase activity using a synthetic peptide (FALGPA) that mimics the structure of collagen. The substrate in this kit is cleaved more efficiently by bacterial collagenases but will be cleaved to some extent by mammalian collagenases and certain MMPs

Collagenase Activity Assay Kit protocol summary:

- Add samples and controls to wells
- Add reaction mix
- Analyze with microplate reader in kinetic mode for 5-15 min

This product is manufactured by BioVision, an Abcam company and was previously called K792 Collagenase Activity Colorimetric Assay Kit. K792-100 is the same size as the 100 test size of ab196999.

Collagenase (EC 3.4.24.3) is an enzyme in the matrix metalloproteinase family that breaks down collagen, assisting in degradation of the extracellular matrix, which is a key step in the pathogenesis of bacteria. Collagen is an abundant structural protein present in the connective tissue of animals. Collagenase has been used clinically for the treatment of Dupuytren's contracture, an affliction characterized by a thickening of connective tissue.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab196999-100Test", "assetComponentDetails": [ { "size":"1 x 4 mL", "name":"Collagenase Substrate I", "number":"AB196999-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"Collagenase", "number":"AB196999-CMP02", "productcode":"" }, { "size":"1 x 50 µL", "name":"Inhibitor (1,10-Phenanthroline)", "number":"AB196999-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Collagenase Assay Buffer", "number":"AB196999-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagenase refers to a group of enzymes capable of breaking down collagen an important structural protein in the extracellular matrix. These enzymes including matrix metalloproteinase (MMP) family members like MMP-1 (also known as fibroblast collagenase) have molecular weights typically around 50-60 kDa. Collagenase enzymes can be expressed in various tissues such as skin cartilage and bone and also in fibroblasts and inflammatory cells. They play a significant role in remodeling and degrading extracellular matrix components which is essential for processes like tissue repair and development.
Biological function summary

These enzymes participate in collagen degradation allowing for cellular migration and growth by clearing path through the dense collagen network. Collagenase activity is carefully regulated because uncontrolled activity can lead to tissue damage and diseases. The enzymes do not function alone; they often form complexes or interact with inhibitors such as tissue inhibitors of metalloproteinases (TIMPs) which maintain the necessary balance in matrix remodeling. Researchers utilize collagenase inhibition assay techniques to study these interactions as well as to gauge enzyme efficiency and control.

Pathways

Collagenase enzymes significantly contribute to the pathways of tissue remodeling and wound healing. They are involved in the matrix metalloproteinase pathway working with other MMPs like MMP-2 and MMP-9. In these pathways they collaborate with proteins such as elastase and stromelysin to degrade various matrix components. Balance between collagenase and TIMP proteins regulates these processes ensuring that tissue integrity is maintained during dynamic changes in the body.

Excessive collagenase activity connects to arthritic disease and tumor metastasis. In arthritis there is an overproduction of collagenase particularly in joints which leads to the excessive breakdown of cartilage. Increased collagenase activity results as well in cancerous tumors enabling them to invade neighboring tissues. Various therapies target the regulation of collagenase and TIMP activity to mitigate tissue destruction and inhibit cancer progression.
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Product protocols

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Target data

Clostridial collagenases are among the most efficient degraders of eukaryotic collagen known; saprophytes use collagen as a carbon source while pathogens additionally digest collagen to aid in host colonization. Has both tripeptidylcarboxypeptidase on Gly-X-Y and endopeptidase activities; the endopeptidase cuts within the triple helix region of collagen while tripeptidylcarboxypeptidase successively digests the exposed ends, thus clostridial collagenases can digest large sections of collagen (PubMed : 3002446). The full-length protein has collagenase activity, while both the 116 kDa and 98 kDa forms act on gelatin (PubMed : 7961400). In vitro digestion of soluble calf skin collagen fibrils requires both ColG and ColH; ColG forms missing the second collagen-binding domain is also synergistic with ColH, although their overall efficiency is decreased (PubMed : 18374061, PubMed : 22099748). Digestion of collagen requires Ca(2+) and is inhibited by EDTA (PubMed : 9452493). The activator domain (residues 119-388) and catalytic subdomain (330-601) open and close around substrate allowing digestion when the protein is closed (PubMed : 23703618).
See full target information colH
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutics 17: PubMed39861784

2025

Biotechnological Phytocomplex of (L.) DC. Enhances Collagen Biosynthesis In Vitro and Improves Skin Elasticity In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanna Rigillo,Giovanna Pressi,Oriana Bertaiola,Chiara Guarnerio,Matilde Merlin,Roberto Zambonin,Stefano Pandolfo,Angela Golosio,Francesca Masin,Fabio Tascedda,Marco Biagi,Giulia Baini

Journal of proteome research 23:3791-3805 PubMed38980715

2024

The Spatial Extracellular Proteomic Tumor Microenvironment Distinguishes Molecular Subtypes of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jade K Macdonald,Harrison B Taylor,Mengjun Wang,Andrew Delacourt,Christin Edge,David N Lewin,Naoto Kubota,Naoto Fujiwara,Fahmida Rasha,Cesia A Marquez,Atsushi Ono,Shiro Oka,Kazuaki Chayama,Sara Lewis,Bachir Taouli,Myron Schwartz,M Isabel Fiel,Richard R Drake,Yujin Hoshida,Anand S Mehta,Peggi M Angel

International journal of molecular sciences 25: PubMed38473981

2024

Skin Anti-Aging Potential through Whitening and Wrinkle Improvement Using Fermented Oil Derived from Larvae.

Applications

Unspecified application

Species

Unspecified reactive species

Dooseon Hwang,Tae-Won Goo,Seung Hun Lee,Eun-Young Yun

Journal of the American Society for Mass Spectrometry 35:487-497 PubMed38329320

2024

Differential Protease Specificity by Collagenase as a Novel Approach to Serum Proteomics That Includes Identification of Extracellular Matrix Proteins without Enrichment.

Applications

Unspecified application

Species

Unspecified reactive species

Jade K Macdonald,Cassandra L Clift,Janet Saunders,Stephen C Zambrzycki,Anand S Mehta,Richard R Drake,Peggi M Angel

Archiv der Pharmazie 357:e2300742 PubMed38290054

2024

Valorization of Citrus peels: GC/MS-based metabolites profiling, multivariate analysis, and antiaging potential.

Applications

Unspecified application

Species

Unspecified reactive species

Wafaa M El Kady,Iriny M Ayoub,Ahmed K El Mehrate,Mohamed Emad,Mohamed Tarek,Ahmed El Gdeily,El-Romisaa Mohamed,Rana Medhat,Omar Mahmoud,Amira M Gad,Alaa M Danderawy,Ola L Breaka,Nada E Mosad,Safaa M Gemeaha,Noha Fathallah

Scientific reports 11:9751 PubMed33963260

2021

Collagen fiber regulation in human pediatric aortic valve development and disease.

Applications

Enzyme activity assay

Species

Bacteria

Cassandra L Clift,Yan Ru Su,David Bichell,Heather C Jensen Smith,Jennifer R Bethard,Kim Norris-Caneda,Susana Comte-Walters,Lauren E Ball,M A Hollingsworth,Anand S Mehta,Richard R Drake,Peggi M Angel

Journal of ginseng research 43:580-588 PubMed31695564

2019

Ginsenoside Rg1 supplementation clears senescence-associated β-galactosidase in exercising human skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Jinfu Wu,Suchada Saovieng,I-Shiung Cheng,Tiemin Liu,Shangyu Hong,Chang-Yu Lin,I-Chen Su,Chih-Yang Huang,Chia-Hua Kuo

Molecules (Basel, Switzerland) 24: PubMed30699941

2019

Ripe and Raw Pu-Erh Tea: LC-MS Profiling, Antioxidant Capacity and Enzyme Inhibition Activities of Aqueous and Hydro-Alcoholic Extracts.

Applications

Enzyme activity assay

Species

Bacteria

Gabriella Roda,Cristina Marinello,Anita Grassi,Claudia Picozzi,Giancarlo Aldini,Marina Carini,Luca Regazzoni
View all publications
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