Collagenase Inhibitor Screening Kit (Fluorometric) (ab211108) provides a quick, sensitive and reliable test for high-throughput screening of collagenase inhibitors.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Fluorescent
Inhibitor compounds
Semi-quantitative
Select an associated product type
CLG, Fibroblast collagenase, Matrix metallopeptidase 1 (interstitial collagenase), matrix metallopeptidase 1
Collagenase Inhibitor Screening Kit (Fluorometric) (ab211108) provides a quick, sensitive and reliable test for high-throughput screening of collagenase inhibitors.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
CLG, Fibroblast collagenase, Matrix metallopeptidase 1 (interstitial collagenase), matrix metallopeptidase 1
Fluorescent
Inhibitor compounds
Semi-quantitative
Microplate reader
Blue Ice
-20°C
-20°C
-20°C
Collagenase Inhibitor Screening Kit (Fluorometric) (ab211108) provides a quick, sensitive and reliable test for high-throughput screening of collagenase inhibitors. The assay uses self-quenched BODIPY conjugate of Gelatin (Type B) as a fluorogenic substrate to monitor the activity of Collagenase. Upon proteolytic digestion of the highly quenched BODIPY-labeled gelatin by Collagenase, the de-quenched BODIPY yields bright green fluorescence that can be assayed using a fluorescence microplate reader at Ex/Em = 490/520 nm (515 nm cutoff). In the presence of a collagenase inhibitor, the gelatin is not digested, the dequenching of BODIPY does not occur and the fluorescent signal is not produced.
This kit can be used to screen/study/characterize potential inhibitors of Collagenase.
This product is manufactured by BioVision, an Abcam company and was previously called K833 Collagenase Inhibitor Screening Kit (Fluorometric). K833-100 is the same size as the 100 test size of ab211108.
Collagenase (EC 3.4.24.3) is an enzyme in the matrix metalloproteinase (MMP) family that breaks down collagen, assisting in degradation of the extracellular matrix, a key step in the pathogenesis of bacteria and tumor cell invasion. Collagen is an abundant structural protein present in the connective tissue of animals. Collagenase has been used clinically for the treatment of Dupuytren's contracture, an affliction characterized by a thickening of connective tissue.
This supplementary information is collated from multiple sources and compiled automatically.
Collagenase refers to a group of enzymes capable of breaking down collagen an important structural protein in the extracellular matrix. These enzymes including matrix metalloproteinase (MMP) family members like MMP-1 (also known as fibroblast collagenase) have molecular weights typically around 50-60 kDa. Collagenase enzymes can be expressed in various tissues such as skin cartilage and bone and also in fibroblasts and inflammatory cells. They play a significant role in remodeling and degrading extracellular matrix components which is essential for processes like tissue repair and development.
These enzymes participate in collagen degradation allowing for cellular migration and growth by clearing path through the dense collagen network. Collagenase activity is carefully regulated because uncontrolled activity can lead to tissue damage and diseases. The enzymes do not function alone; they often form complexes or interact with inhibitors such as tissue inhibitors of metalloproteinases (TIMPs) which maintain the necessary balance in matrix remodeling. Researchers utilize collagenase inhibition assay techniques to study these interactions as well as to gauge enzyme efficiency and control.
Collagenase enzymes significantly contribute to the pathways of tissue remodeling and wound healing. They are involved in the matrix metalloproteinase pathway working with other MMPs like MMP-2 and MMP-9. In these pathways they collaborate with proteins such as elastase and stromelysin to degrade various matrix components. Balance between collagenase and TIMP proteins regulates these processes ensuring that tissue integrity is maintained during dynamic changes in the body.
Excessive collagenase activity connects to arthritic disease and tumor metastasis. In arthritis there is an overproduction of collagenase particularly in joints which leads to the excessive breakdown of cartilage. Increased collagenase activity results as well in cancerous tumors enabling them to invade neighboring tissues. Various therapies target the regulation of collagenase and TIMP activity to mitigate tissue destruction and inhibit cancer progression.
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Collagenase Inhibitor Screening Kit (ab211108). Typical inhibition curve of Collagenase activity by collagenase inhibitor. IC50 of (1,10)-Phenanthroline was determine to be 110.5 μM. Assay was performed following the kit protocol.
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