Collagenase Inhibitor Screening Kit (Fluorometric) (ab211108) provides a quick, sensitive and reliable test for high-throughput screening of collagenase inhibitors.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Images
Publications
Filter by
- International journal of molecular sciences 24:2023Comparative Study of Cytotoxicity and Antioxidant, Anti-Aging and Antibacterial Properties of Unfermented and Fermented Extract of L.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartyna Zagórska-Dziok,Aleksandra Ziemlewska,Agnieszka Mokrzyńska,Zofia Nizioł-Łukaszewska,Ireneusz Sowa,Dariusz Szczepanek,Magdalena WójciakPubMed 37686038
- Molecules (Basel, Switzerland) 28:2023Phenolic Profile and Comparison of the Antioxidant, Anti-Ageing, Anti-Inflammatory, and Protective Activities of Extracts on Skin Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMonika Michalak,Martyna Zagórska-Dziok,Marta Klimek-Szczykutowicz,Agnieszka SzopaPubMed 36677923
- Molecules (Basel, Switzerland) 27:2022A New Xanthone Glycoside from L.: Physicochemical Properties and In Vitro Anti-Skin Aging Activities.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeba A S El-Nashar,Eman M El-Labbad,Mahmood A Al-Azzawi,Naglaa S AshmawyPubMed 35565960
- Molecules (Basel, Switzerland) 27:2022Evaluation of Cosmetic and Dermatological Properties of Kombucha-Fermented Berry Leaf Extracts Considered to Be By-Products.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAleksandra Ziemlewska,Zofia Nizioł-Łukaszewska,Martyna Zagórska-Dziok,Tomasz Bujak,Magdalena Wójciak,Ireneusz SowaPubMed 35408743
- Molecules (Basel, Switzerland) 27:2022Flower Extracts as Multifunctional Dyes in the Cosmetics Industry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomasz Bujak,Martyna Zagórska-Dziok,Aleksandra Ziemlewska,Zofia Nizioł-Łukaszewska,Kamila Lal,Tomasz Wasilewski,Zofia Hordyjewicz-BaranPubMed 35164187
- Scientific reports 11:187922021Effect of fermentation time on the content of bioactive compounds with cosmetic and dermatological properties in Kombucha Yerba Mate extracts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAleksandra Ziemlewska,Zofia Nizioł-Łukaszewska,Tomasz Bujak,Martyna Zagórska-Dziok,Magdalena Wójciak,Ireneusz SowaPubMed 34552134
- Molecules (Basel, Switzerland) 26:2021L. Extracts as Valuable Ingredients in Cosmetic and Dermatological Products.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Nowak,Martyna Zagórska-Dziok,Paula Ossowicz-Rupniewska,Edyta Makuch,Wiktoria Duchnik,Łukasz Kucharski,Urszula Adamiak-Giera,Piotr Prowans,Norbert Czapla,Piotr Bargiel,Jan Petriczko,Marta Markowska,Adam KlimowiczPubMed 34200200
- Molecules (Basel, Switzerland) 26:2021Antioxidant and Cytoprotective Properties of Plant Extract from Dry Flowers as Functional Dyes for Cosmetic Products.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomasz Bujak,Martyna Zagórska-Dziok,Aleksandra Ziemlewska,Zofia Nizioł-Łukaszewska,Tomasz Wasilewski,Zofia Hordyjewicz-BaranPubMed 34068613
- Molecules (Basel, Switzerland) 26:2021Positive Effect of L. Herb Extracts on Skin Cells and Assessment of Cannabinoid-Based Hydrogels Properties.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartyna Zagórska-Dziok et. al.PubMed 33557174
- Molecules (Basel, Switzerland) 26:2021Cosmetic and Dermatological Properties of Selected Ayurvedic Plant Extracts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartyna Zagórska-Dziok et. al.PubMed 33503925
Key facts
- Detection method
Fluorescent
- Sample types
Inhibitor compounds
- Assay type
Semi-quantitative
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Alternative names
CLG, Fibroblast collagenase, Matrix metallopeptidase 1 (interstitial collagenase), matrix metallopeptidase 1
What's included?
Recommended products
Collagenase Inhibitor Screening Kit (Fluorometric) (ab211108) provides a quick, sensitive and reliable test for high-throughput screening of collagenase inhibitors.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
CLG, Fibroblast collagenase, Matrix metallopeptidase 1 (interstitial collagenase), matrix metallopeptidase 1
Key facts
- Detection method
Fluorescent
- Sample types
Inhibitor compounds
- Assay type
Semi-quantitative
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Collagenase Inhibitor Screening Kit (Fluorometric) (ab211108) provides a quick, sensitive and reliable test for high-throughput screening of collagenase inhibitors. The assay uses self-quenched BODIPY conjugate of Gelatin (Type B) as a fluorogenic substrate to monitor the activity of Collagenase. Upon proteolytic digestion of the highly quenched BODIPY-labeled gelatin by Collagenase, the de-quenched BODIPY yields bright green fluorescence that can be assayed using a fluorescence microplate reader at Ex/Em = 490/520 nm (515 nm cutoff). In the presence of a collagenase inhibitor, the gelatin is not digested, the dequenching of BODIPY does not occur and the fluorescent signal is not produced.
This kit can be used to screen/study/characterize potential inhibitors of Collagenase.
This product is manufactured by BioVision, an Abcam company and was previously called K833 Collagenase Inhibitor Screening Kit (Fluorometric). K833-100 is the same size as the 100 test size of ab211108.
Collagenase (EC 3.4.24.3) is an enzyme in the matrix metalloproteinase (MMP) family that breaks down collagen, assisting in degradation of the extracellular matrix, a key step in the pathogenesis of bacteria and tumor cell invasion. Collagen is an abundant structural protein present in the connective tissue of animals. Collagenase has been used clinically for the treatment of Dupuytren's contracture, an affliction characterized by a thickening of connective tissue.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Collagenase refers to a group of enzymes capable of breaking down collagen an important structural protein in the extracellular matrix. These enzymes including matrix metalloproteinase (MMP) family members like MMP-1 (also known as fibroblast collagenase) have molecular weights typically around 50-60 kDa. Collagenase enzymes can be expressed in various tissues such as skin cartilage and bone and also in fibroblasts and inflammatory cells. They play a significant role in remodeling and degrading extracellular matrix components which is essential for processes like tissue repair and development.
- Biological function summary
These enzymes participate in collagen degradation allowing for cellular migration and growth by clearing path through the dense collagen network. Collagenase activity is carefully regulated because uncontrolled activity can lead to tissue damage and diseases. The enzymes do not function alone; they often form complexes or interact with inhibitors such as tissue inhibitors of metalloproteinases (TIMPs) which maintain the necessary balance in matrix remodeling. Researchers utilize collagenase inhibition assay techniques to study these interactions as well as to gauge enzyme efficiency and control.
- Pathways
Collagenase enzymes significantly contribute to the pathways of tissue remodeling and wound healing. They are involved in the matrix metalloproteinase pathway working with other MMPs like MMP-2 and MMP-9. In these pathways they collaborate with proteins such as elastase and stromelysin to degrade various matrix components. Balance between collagenase and TIMP proteins regulates these processes ensuring that tissue integrity is maintained during dynamic changes in the body.
- Associated diseases and disorders
Excessive collagenase activity connects to arthritic disease and tumor metastasis. In arthritis there is an overproduction of collagenase particularly in joints which leads to the excessive breakdown of cartilage. Increased collagenase activity results as well in cancerous tumors enabling them to invade neighboring tissues. Various therapies target the regulation of collagenase and TIMP activity to mitigate tissue destruction and inhibit cancer progression.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Functional Studies - Collagenase Inhibitor Assay Kit (Fluorometric) (ab211108)
Collagenase Inhibitor Screening Kit (ab211108). Typical inhibition curve of Collagenase activity by collagenase inhibitor. IC50 of (1,10)-Phenanthroline was determine to be 110.5 μM. Assay was performed following the kit protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com