Collagenase Inhibitor Assay Kit (Fluorometric)
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(13 Publications)
Collagenase Inhibitor Screening Kit (Fluorometric) (ab211108) provides a quick, sensitive and reliable test for high-throughput screening of collagenase inhibitors.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
View Alternative Names
CLG, Fibroblast collagenase, Matrix metallopeptidase 1 (interstitial collagenase), matrix metallopeptidase 1
- FuncS
Supplier Data
Functional Studies - Collagenase Inhibitor Assay Kit (Fluorometric) (AB211108)
Collagenase Inhibitor Screening Kit (ab211108). Typical inhibition curve of Collagenase activity by collagenase inhibitor. IC50 of (1,10)-Phenanthroline was determine to be 110.5 μM. Assay was performed following the kit protocol.
Reactivity data
Product details
Collagenase Inhibitor Screening Kit (Fluorometric) (ab211108) provides a quick, sensitive and reliable test for high-throughput screening of collagenase inhibitors. The assay uses self-quenched BODIPY conjugate of Gelatin (Type B) as a fluorogenic substrate to monitor the activity of Collagenase. Upon proteolytic digestion of the highly quenched BODIPY-labeled gelatin by Collagenase, the de-quenched BODIPY yields bright green fluorescence that can be assayed using a fluorescence microplate reader at Ex/Em = 490/520 nm (515 nm cutoff). In the presence of a collagenase inhibitor, the gelatin is not digested, the dequenching of BODIPY does not occur and the fluorescent signal is not produced.
This kit can be used to screen/study/characterize potential inhibitors of Collagenase.
Other Notes
This product was previously called K833 Biovision Collagenase Inhibitor Screening Kit (Fluorometric). Biovision was acquired by Abcam in 2021.
Collagenase (EC 3.4.24.3) is an enzyme in the matrix metalloproteinase (MMP) family that breaks down collagen, assisting in degradation of the extracellular matrix, a key step in the pathogenesis of bacteria and tumor cell invasion. Collagen is an abundant structural protein present in the connective tissue of animals. Collagenase has been used clinically for the treatment of Dupuytren's contracture, an affliction characterized by a thickening of connective tissue.
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Properties and storage information
Shipped at conditions
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Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
These enzymes participate in collagen degradation allowing for cellular migration and growth by clearing path through the dense collagen network. Collagenase activity is carefully regulated because uncontrolled activity can lead to tissue damage and diseases. The enzymes do not function alone; they often form complexes or interact with inhibitors such as tissue inhibitors of metalloproteinases (TIMPs) which maintain the necessary balance in matrix remodeling. Researchers utilize collagenase inhibition assay techniques to study these interactions as well as to gauge enzyme efficiency and control.
Pathways
Collagenase enzymes significantly contribute to the pathways of tissue remodeling and wound healing. They are involved in the matrix metalloproteinase pathway working with other MMPs like MMP-2 and MMP-9. In these pathways they collaborate with proteins such as elastase and stromelysin to degrade various matrix components. Balance between collagenase and TIMP proteins regulates these processes ensuring that tissue integrity is maintained during dynamic changes in the body.
Product protocols
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Publications (13)
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International journal of molecular sciences 25: PubMed38892121
2024
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International journal of molecular sciences 24: PubMed37686038
2023
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Molecules (Basel, Switzerland) 28: PubMed36677923
2023
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Molecules (Basel, Switzerland) 27: PubMed35565960
2022
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Molecules (Basel, Switzerland) 27: PubMed35408743
2022
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Molecules (Basel, Switzerland) 27: PubMed35164187
2022
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Scientific reports 11:18792 PubMed34552134
2021
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Molecules (Basel, Switzerland) 26: PubMed34200200
2021
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Molecules (Basel, Switzerland) 26: PubMed34068613
2021
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Molecules (Basel, Switzerland) 26: PubMed33557174
2021
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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