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Optimized for isolation of Complex I from small amounts of tissue for activity assays and other analyses.


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Key facts

Assay type

Quantitative

Reactive species

Mouse, Rat, Cow, Human

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What's included?

250 µg
Components
Complex 1 monoclonal antibody coupled agarose beads
2 x 250 µg

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Optimized for isolation of Complex I from small amounts of tissue for activity assays and other analyses.

Key facts

Assay type

Quantitative

Reactive species

Mouse, Rat, Cow, Human

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Optimized for isolation of Complex I from small amounts of tissue for activity assays and other analyses. Based on a highly specific antibody.

250 μg, 500 μg or 750 μg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~60 μg, ~120 μg or ~180 μg respectively of Complex I from heart mitochondria.

ab109711 allows for isolation of Complex I from small amounts of tissue. This facilitates subsequent analysis of assembly state and activity. Thus the enzyme retains NADH-ferricyanide, NADH-CoQ1 and NADH hexaaminoruthenium reductase activities after isolation, and with added lipids it also shows significant rotenone sensitivity. Finally, the extent of post translational modifications including oxidative damage can be readily analyzed by proteomic approaches or antibody detection of these modifications. Uses for ab109711 include but are not limited to examining alterations of Complex I subunits in inherited mitochondrial diseases, Parkinson's disease, Alzheimer's disease, ALS, schizophrenia, and aging.

Note: The immunocapture protocol for this kit requires Abcam detergent (lauryl maltoside, ab109857).

Product supplied as a 6.25% slurry. For the 250 μg, this consists of 250 μg antibody conjugated to 25 μL beads in a volume of 250 μL.

**Related products** Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

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1 product image

  • Immunoprecipitation - Complex I Immunocapture Kit (ab109711), expandable thumbnail

    Immunoprecipitation - Complex I Immunocapture Kit (ab109711)

    Complex I was immunopurified from mitochondria isolated from human heart (HHM), cow/bovine heart (BHM), mouse heart (MHM) and mouse brain (MBM). The lanes were stained with Coomassie Brilliant Blue R. Bands were excised from the gel and proteolytically digested for mass spectrometry analysis."

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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