Complex I Immunocapture Kit

Optimized for isolation of Complex I from small amounts of tissue for activity assays and other analyses. Based on a highly specific antibody.

250 μg, 500 μg or 750 μg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~60 μg, ~120 μg or ~180 μg respectively of Complex I from heart mitochondria.

ab109711 allows for isolation of Complex I from small amounts of tissue. This facilitates subsequent analysis of assembly state and activity. Thus the enzyme retains NADH-ferricyanide, NADH-CoQ1 and NADH hexaaminoruthenium reductase activities after isolation, and with added lipids it also shows significant rotenone sensitivity. Finally, the extent of post translational modifications including oxidative damage can be readily analyzed by proteomic approaches or antibody detection of these modifications. Uses for ab109711 include but are not limited to examining alterations of Complex I subunits in inherited mitochondrial diseases, Parkinson's disease, Alzheimer's disease, ALS, schizophrenia, and aging.

Note: The immunocapture protocol for this kit requires Abcam detergent (lauryl maltoside, ab109857).

Product supplied as a 6.25% slurry. For the 250 μg, this consists of 250 μg antibody conjugated to 25 μL beads in a volume of 250 μL.

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