Optimized for isolation of Complex I from small amounts of tissue for activity assays and other analyses.
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- Journal of neurochemistry 167:218-2472023Neuroanatomical zones of human traumatic brain injury reveal significant differences in protein profile and protein oxidation: Implications for secondary injury events.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNiya Gowthami,Nithya Pursotham,Gourav Dey,Vivek Ghose,Gajanan Sathe,Nupur Pruthi,Dhaval Shukla,Narayanappa Gayathri,Rashmi Santhoshkumar,Balasundaram Padmanabhan,Vivek Chandramohan,Anita Mahadevan,M M Srinivas BharathPubMed 37694499
- Communications biology 5:6202022Most mitochondrial dGTP is tightly bound to respiratory complex I through the NDUFA10 subunit.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid Molina-Granada,Emiliano González-Vioque,Marris G Dibley,Raquel Cabrera-Pérez,Antoni Vallbona-Garcia,Javier Torres-Torronteras,Leonid A Sazanov,Michael T Ryan,Yolanda Cámara,Ramon MartíPubMed 35739187
- Developmental cell 57:260-276.e92022Regulation of reverse electron transfer at mitochondrial complex I by unconventional Notch action in cancer stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRani Ojha,Ishaq Tantray,Suman Rimal,Siddhartha Mitra,Sam Cheshier,Bingwei LuPubMed 35077680
- Scientific reports 11:194082021DJ-1 attenuates the glycation of mitochondrial complex I and complex III in the post-ischemic heart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYvanna Pantner,Rohini Polavarapu,Lih-Shen Chin,Lian Li,Yuuki Shimizu,John W CalvertPubMed 34593886
- Cell reports 35:1090022021NDUFS3 depletion permits complex I maturation and reveals TMEM126A/OPA7 as an assembly factor binding the ND4-module intermediate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuigi D'Angelo,Elisa Astro,Monica De Luise,Ivana Kurelac,Nikkitha Umesh-Ganesh,Shujing Ding,Ian M Fearnley,Giuseppe Gasparre,Massimo Zeviani,Anna Maria Porcelli,Erika Fernandez-Vizarra,Luisa IommariniPubMed 33882309
- Journal of medicinal chemistry 63:11819-118302020Characterization and Discovery of a Selective Small-Molecule Modulator of Mitochondrial Complex I Targeting a Unique Binding Site.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJakob C Green et. al.PubMed 32945676
- Redox biology 37:1017052020Insight into the mechanism of cytotoxicity of membrane-permeant psoralenic Kv1.3 channel inhibitors by chemical dissection of a novel member of the family.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoberta Peruzzo et. al.PubMed 33007503
- Mitochondrion 49:73-822019Complex I and II are required for normal mitochondrial Ca homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFabian Jaña et. al.PubMed 31310854
- Cell chemical biology 25:413-425.e62018Switch of Mitochondrial Superoxide Dismutase into a Prooxidant Peroxidase in Manganese-Deficient Cells and Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDouglas Ganini,Janine H Santos,Marcelo G Bonini,Ronald P MasonPubMed 29398562
- Antioxidants & redox signaling 28:44-612017Mitochondrial Complex I Reversible S-Nitrosation Improves Bioenergetics and Is Protective in Parkinson's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChiara Milanese,Victor Tapias,Sylvia Gabriels,Silvia Cerri,Giovanna Levandis,Fabio Blandini,Maria Tresini,Sruti Shiva,John Timothy Greenamyre,Mark T Gladwin,Pier G MastroberardinoPubMed 28816057
Key facts
- Assay type
- Quantitative
- Reactive species
- Mouse, Rat, Cow, Human
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Optimized for isolation of Complex I from small amounts of tissue for activity assays and other analyses.
Key facts
- Assay type
- Quantitative
- Reactive species
- Mouse, Rat, Cow, Human
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Optimized for isolation of Complex I from small amounts of tissue for activity assays and other analyses. Based on a highly specific antibody.
250 μg, 500 μg or 750 μg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~60 μg, ~120 μg or ~180 μg respectively of Complex I from heart mitochondria.
ab109711 allows for isolation of Complex I from small amounts of tissue. This facilitates subsequent analysis of assembly state and activity. Thus the enzyme retains NADH-ferricyanide, NADH-CoQ1 and NADH hexaaminoruthenium reductase activities after isolation, and with added lipids it also shows significant rotenone sensitivity. Finally, the extent of post translational modifications including oxidative damage can be readily analyzed by proteomic approaches or antibody detection of these modifications. Uses for ab109711 include but are not limited to examining alterations of Complex I subunits in inherited mitochondrial diseases, Parkinson's disease, Alzheimer's disease, ALS, schizophrenia, and aging.
Note: The immunocapture protocol for this kit requires Abcam detergent (lauryl maltoside, 10% Lauryl Maltoside Solution ab109857).
Product supplied as a 6.25% slurry. For the 250 μg, this consists of 250 μg antibody conjugated to 25 μL beads in a volume of 250 μL.
**Related products** Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Complex I also known as NADH dehydrogenase or NADH:ubiquinone oxidoreductase is a large enzyme complex with a molecular mass of approximately 1000 kDa. It is expressed in the inner mitochondrial membrane of eukaryotic cells. As the first enzyme in the mitochondrial respiratory chain Complex I plays a critical mechanical role in cellular respiration. It transfers electrons from NADH to ubiquinone coupled with the translocation of protons across the inner mitochondrial membrane contributing to the generation of a proton gradient used to produce ATP.
- Biological function summary
Complex I acts as an integral component of the mitochondrial respiratory chain which is a series of protein complexes involved in cellular energy production. As part of this complex system Complex I is essential for effective oxidative phosphorylation. Its activity is assessed using protein activity assays including immunocapture or complex activity assays and microplate assays. Complex I activity influences the overall efficiency of ATP production affecting energy-dependent cellular processes.
- Pathways
Complex I functions within the electron transport chain one of the major pathways in cellular respiration. This pathway is vital for ATP synthesis providing the energy currency required by cells. Complex I works closely with other electron transport chain complexes such as Complex II (succinate dehydrogenase complex) and Complex III (cytochrome c reductase) to drive oxidation-reduction reactions and maintain cellular metabolism.
- Associated diseases and disorders
Defects in Complex I are linked with mitochondrial diseases and neurodegenerative disorders such as Leigh syndrome and Parkinson's disease. Mutations in Complex I subunits disrupt normal electron transport and ATP production leading to increased oxidative stress and neuronal cell damage. Other mitochondrial proteins such as cytochrome c oxidase are also implicated in these conditions emphasizing the interconnected nature of mitochondrial dysfunction in disease progression.
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Immunoprecipitation - Complex I Immunocapture Kit (ab109711)
Complex I was immunopurified from mitochondria isolated from human heart (HHM), cow/bovine heart (BHM), mouse heart (MHM) and mouse brain (MBM). The lanes were stained with Coomassie Brilliant Blue R. Bands were excised from the gel and proteolytically digested for mass spectrometry analysis."
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