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Complex II Enzyme Activity Microplate Assay Kit is designed for determining the Complex II activity in a human, mouse, rat or bovine sample.

Detection method

Colorimetric

Sample types

Tissue Lysate, Purified mitochondria, Cell Lysate

Assay type

Enzyme activity

Reactive species

Mouse, Rat, Cow, Human

Assay time

3h

Images

Target data

What's included?

96 Test
Components
10X Blocking Solution
1 x 5 mL
20X Buffer
1 x 15 mL
Complex II Activity Buffer
1 x 25 mL
DCPIP/DCIP
1 x 250 µL
Detergent
2 x 1 mL
Phospholipids
1 x 6 mL
Pre-coated 96-well microplate (12 strips)
1 x 1 Unit
Succinate
1 x 500 µL
Ubiquinone 2
1 x 60 µL

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Complex II Enzyme Activity Microplate Assay Kit is designed for determining the Complex II activity in a human, mouse, rat or bovine sample.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Purified mitochondria, Cell Lysate

Assay type

Enzyme activity

Reactive species

Mouse, Rat, Cow, Human

Assay time

3h

Assay Platform

Microplate reader

Storage

Shipped at conditions

Dry Ice

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Complex II Enzyme Activity Microplate Assay Kit is designed for determining the Complex II activity in a human, mouse, rat or bovine sample. Each of the 96 wells in the kit has been coated with an anti-Complex II monoclonal antibody (mAb) which purifies the enzyme from a complex sample such as mitochondria, tissue homogenate or cell lysate. After this in-well purification the production of ubiquinol by the enzyme is coupled to the reduction of the dye DCPIP (2,6-diclorophenolindophenol) and a decreases in its absorbance at 600 nm, which in turn recycles the substrate ubiquinone.

Succinate, Ubiquinone 2, DCPIP and Phospholipids should be stored at -80°C. All other components should be stored at 4°C. **Related products** Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

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Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

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