Complex II Immunocapture Kit
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(7 Publications)
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 µg, ~26 µg or ~39 µg respectively of Complex II from heart mitochondria.
View Alternative Names
SDH2, SDHF, SDHA, Flavoprotein subunit of complex II, Malate dehydrogenase [quinone] flavoprotein subunit, Fp
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Supplier Data
Immunoprecipitation - Complex II Immunocapture Kit (AB109799)
Complex II immunoprecipitation using antibody ab109865 crosslinked to protein G-agarose beads as product ab109799. Complex II was immunoprecipitated from a lauryl maltoside detergent extract of 1000 ug bovine heart mitochondria or 500 ug human heart mitochondria. The gel was stained with coomassie brilliant blue.
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Product details
250 μg, 500 μg or 750 μg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 μg, ~26 μg or ~39 μg respectively of Complex II from heart mitochondria. Isolated mitochondria are available from several species for use as control samples.
The Complex II Immunocapture Kit allows isolation of the succinate dehydrogenase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for the Complex II immunocapture kit include research on aging genetic mitochondrial disease, and various cancers due to mutations in this enzyme complex such as paragangliomas and phaeo-chromocytomas.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).
**Related products**Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
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Biological function summary
Complex II functions as part of the larger electron transport chain complex and plays a role in the Krebs cycle. It links two critical metabolic pathways converting succinate to fumarate while transferring electrons to the electron transport chain. This makes it integral for proper cellular respiration and energy production. The complex consists of multiple subunits and utilizes co-factors like FAD and iron-sulfur clusters for enzymatic activity. It is also a part of the supercomplexes that optimize the efficiency of oxidative phosphorylation.
Pathways
Complex II plays a significant role in both the citric acid cycle and oxidative phosphorylation. It acts as a connecting bridge between these two pathways facilitating the flow of electrons. Complex II works alongside other proteins such as complex I and complex III to maintain the electron transport chain's function and energy production. Succinate dehydrogenase transfers electrons within the chain directly affecting the generation of ATP by complex V (ATP synthase).
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Publications (7)
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Molecular metabolism 29:40-54 PubMed31668391
2019
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Mitochondrion 49:73-82 PubMed31310854
2019
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American journal of human genetics 91:729-36 PubMed23022099
2012
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PloS one 6:e23295 PubMed21858060
2011
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Mouse
Advances in experimental medicine and biology 648:361-8 PubMed19536500
2009
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Proceedings of the National Academy of Sciences of 105:14447-52 PubMed18794531
2008
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Biochimica et biophysica acta 1762:213-22 PubMed16120479
2005
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