250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 µg, ~26 µg or ~39 µg respectively of Complex II from heart mitochondria.
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- Molecular metabolism 29:40-542019miR-873-5p targets mitochondrial GNMT-Complex II interface contributing to non-alcoholic fatty liver disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPablo Fernández-Tussy et. al.PubMed 31668391
- Mitochondrion 49:73-822019Complex I and II are required for normal mitochondrial Ca homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFabian Jaña et. al.PubMed 31310854
- American journal of human genetics 91:729-362012Infantile encephaloneuromyopathy and defective mitochondrial translation are due to a homozygous RMND1 mutation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBeatriz Garcia-Diaz et. al.PubMed 23022099
- PloS one 6:e232952011Succinate dehydrogenase is a direct target of sirtuin 3 deacetylase activity.
- Advances in experimental medicine and biology 648:361-82009Impact of modulators of mitochondrial ATP-sensitive potassium channel (mitoK(ATP)) on hypoxic pulmonary vasoconstriction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesR Paddenberg,P Faulhammer,A Goldenberg,B Gries,J Heinl,W KummerPubMed 19536500
- Proceedings of the National Academy of Sciences of 105:14447-522008A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBong-Hyun Ahn et. al.PubMed 18794531
- Biochimica et biophysica acta 1762:213-222005Proteomic analysis of succinate dehydrogenase and ubiquinol-cytochrome c reductase (Complex II and III) isolated by immunoprecipitation from bovine and mouse heart mitochondria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBirgit Schilling,James Murray,Chris B Yoo,Richard H Row,Michael P Cusack,Roderick A Capaldi,Bradford W GibsonPubMed 16120479
Key facts
- Assay type
Quantitative
- Reactive species
Mouse, Rat, Cow, Human
Associated Products
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Target data
Function
Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA (PubMed:24766809, PubMed:24766810). Its role in tRNA splicing and maturation is required for cerebellar development (PubMed:24766809, PubMed:24766810). Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.
Targets
SDHA
Alternative names
Polyribonucleotide 5'-hydroxyl-kinase Clp1, Polyadenylation factor Clp1, Polynucleotide kinase Clp1, Pre-mRNA cleavage complex II protein Clp1, HEAB, CLP1
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250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 µg, ~26 µg or ~39 µg respectively of Complex II from heart mitochondria.
Key facts
- Assay type
Quantitative
- Reactive species
Mouse, Rat, Cow, Human
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
250 μg, 500 μg or 750 μg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 μg, ~26 μg or ~39 μg respectively of Complex II from heart mitochondria. Isolated mitochondria are available from several species for use as control samples.
The Complex II Immunocapture Kit allows isolation of the succinate dehydrogenase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for the Complex II immunocapture kit include research on aging genetic mitochondrial disease, and various cancers due to mutations in this enzyme complex such as paragangliomas and phaeo-chromocytomas.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (10% Lauryl Maltoside Solution ab109857/MS910).
**Related products**Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Complex II also known as succinate dehydrogenase (SDH) or succinate-ubiquinone oxidoreductase plays an important mechanical role in the mitochondrial electron transport chain. It catalyzes the oxidation of succinate to fumarate while reducing ubiquinone to ubiquinol. The enzyme complex is approximately 140 kDa in mass and resides in the inner mitochondrial membrane. Complex II is expressed in most tissues particularly in high-energy demand tissues such as the heart and skeletal muscles.
- Biological function summary
Complex II functions as part of the larger electron transport chain complex and plays a role in the Krebs cycle. It links two critical metabolic pathways converting succinate to fumarate while transferring electrons to the electron transport chain. This makes it integral for proper cellular respiration and energy production. The complex consists of multiple subunits and utilizes co-factors like FAD and iron-sulfur clusters for enzymatic activity. It is also a part of the supercomplexes that optimize the efficiency of oxidative phosphorylation.
- Pathways
Complex II plays a significant role in both the citric acid cycle and oxidative phosphorylation. It acts as a connecting bridge between these two pathways facilitating the flow of electrons. Complex II works alongside other proteins such as complex I and complex III to maintain the electron transport chain's function and energy production. Succinate dehydrogenase transfers electrons within the chain directly affecting the generation of ATP by complex V (ATP synthase).
- Associated diseases and disorders
Dysfunction of complex II is associated with mitochondrial diseases and cancers. Mutations or deficiencies in its subunits can lead to conditions like Leigh syndrome and hereditary paraganglioma. These conditions frequently involve other mitochondrial proteins and complexes such as complex I which can exacerbate the electron transport chain dysfunction. In cancers alterations in succinate dehydrogenase activity can result in oncogenic metabolisms by falsely stabilizing hypoxia-inducible factors linking it further with the genetic and metabolic regulation.
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Immunoprecipitation - Complex II Immunocapture Kit (ab109799)
Complex II immunoprecipitation using antibody Anti-Complex II Immunocapture antibody [4H12BG12AG2] ab109865 crosslinked to protein G-agarose beads as product ab109799. Complex II was immunoprecipitated from a lauryl maltoside detergent extract of 1000 ug bovine heart mitochondria or 500 ug human heart mitochondria. The gel was stained with coomassie brilliant blue.
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