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Complex IV Rodent Enzyme Activity Microplate Assay Kit (ab109911) is used to determine the activity of cytochrome c oxidase in a mouse sample with speed and simplicity.

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue

Assay type

Enzyme activity (quantitative)

Reactive species

Mouse, Rat

Assay time

6h

Images

Target data

What's included?

96 Test
Components
96-well microplate
1 x 1 Unit
Detergent
1 x 1 mL
Reagent C (Reduced Cytochrome c)
1 x 1 mL
Tube 1 (Buffer)
1 x 10 mL

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Complex IV Rodent Enzyme Activity Microplate Assay Kit (ab109911) is used to determine the activity of cytochrome c oxidase in a mouse sample with speed and simplicity.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue

Assay type

Enzyme activity (quantitative)

Reactive species

Mouse, Rat

Assay time

6h

Assay Platform

Microplate reader

Storage

Shipped at conditions

Dry Ice

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Complex IV Rodent Enzyme Activity Microplate Assay Kit (ab109911) is used to determine the activity of cytochrome c oxidase in a mouse sample with speed and simplicity. The COX enzyme is immunocaptured within the wells of the microplate and activity is determined colorimetrically by following the oxidation of reduced cytochrome c by the absorbance change at 550 nm. Included in this kit for performance of the activity assay are buffer, detergent, substrate, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

Complex IV assay protocol summary:
- add samples to wells to capture enzyme and incubate for 3 hrs
- wash wells
- add reaction mix
- analyze with microplate reader in kinetic mode for 120 min

Buffer, detergent, and microplate should be stored at 4°C, Reagent C (reduced cytochrome c) should be stored at -80°C.

**Range of complex IV / cytochrome c oxidase assay kits**Biochemical assay - Immunocapture with biochemical assay (plate-based)*** - (this kit) and *** Most popular assay formatImmunocapture with biochemical assay (dipstick) - and Immunocapture with biochemical assay and ELISA - ELISA - **Other related products**Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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