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AB272528

Copper Assay Kit

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(6 Publications)

Simple, direct and automation-ready procedure for measuring copper concentrations in a wide range of applications in research, drug discovery and environmental monitoring.
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Functional Studies - Copper Assay Kit (AB272528)
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Supplier Data

Functional Studies - Copper Assay Kit (AB272528)

Copper Assay Kit Standard Curve.

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Other biological fluids

Results type

Quantitative

Range

7 - 300 µg/dL

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Product details

Simple, direct and automation-ready procedure for measuring copper concentrations in a wide range of applications in research, drug discovery and environmental monitoring. This copper assay kit is designed to measure copper with no or minimal sample treatment. The improved method utilizes a chromogen that forms a colored complex specifically with copper ions. The intensity of the color, measured at 359nm, is directly proportional to copper concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

Sensitive and accurate. Linear detection range 7 μg/dL (1.0 μM) to 300 μg/dL (47 μM) copper in 96-well plate assay.

Simple and high-throughput. The simple procedure can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.

Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.

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What's included?

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Copper is a trace element essential for various enzymatic reactions also known as a transition metal. It is an important component of several metalloproteins and enzymes such as cytochrome c oxidase with a molecular mass ranging from 6000 to 16000 Daltons for its various bound forms. Copper expresses in tissues throughout the body particularly in the liver brain and kidneys. Analytical tools like copper assay kits and copper stain histology identify and quantify its presence within biological samples.
Biological function summary

Copper is integral to processes like oxidative phosphorylation and free radical detoxification. It functions within multi-subunit enzyme complexes aiding electron transfer and free radical scavenging. Copper-dependent enzymes such as superoxide dismutase and lysyl oxidase require copper for activity showcasing its role in cellular defense mechanisms and connective tissue biogenesis. Copper measurement is critical to assess its biological impact on these processes.

Pathways

Copper is involved in mitochondrial respiration and the electron transport chain important parts of energy metabolism. Copper influences pathways by participating in the electron transfer as part of cytochrome c oxidase and regulating the activity of mitochondrial cytochromes. It is closely related to proteins such as ATP7A and ATP7B which manage its cellular distribution and export integral to maintaining copper homeostasis in metabolic pathways.

Copper imbalance can lead to conditions like Wilson's disease and Menkes disease. Excessive copper accumulation in organs causes Wilson's disease attributed to mutations affecting copper-transporting ATPase (ATP7B) proteins. Menkes disease results from deficient copper absorption linked to ATP7A protein issues highlighting copper's importance in metabolic disorders. Copper test kits and copper staining aid in diagnosing and understanding these pathologies.
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Product protocols

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Target data

See full target information Copper
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40362619

2025

Rescue of the First Mitochondrial Membrane Carrier, the mPiC, by TAT-Mediated Protein Replacement Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Samar Zabit,Orly Melloul,Michal Lichtenstein,Erin L Seifert,Haya Lorberboum-Galski

Frontiers in immunology 16:1560322 PubMed40248707

2025

Cuproptosis-related gene ACAD8 inhibits the metastatic ability of colorectal cancer by inducing cuproptosis.

Applications

Unspecified application

Species

Unspecified reactive species

HuiE Zhuang,Yizhen Chen,Sifu Huang

Clinical and translational medicine 14:e70100 PubMed39581695

2024

CircSpna2 attenuates cuproptosis by mediating ubiquitin ligase Keap1 to regulate the Nrf2-Atp7b signalling axis in depression after traumatic brain injury in a mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Mengran Du,Jiayuanyuan Fu,Jie Zhang,Ziyu Zhu,Xuekang Huang,Weilin Tan,Lian Liu,Zhijian Huang,Xin Liu,Qiuhao Tan,ZhengBu Liao,Yuan Cheng

Bioscience trends 17:381-392 PubMed37866883

2023

The function and immune role of cuproptosis associated hub gene in Barrett's esophagus and esophageal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kailin Lin,Ke Hu,Qiwen Chen,Jiangchun Wu

Cells 12: PubMed37048114

2023

Inhibition of LRRK2 Attenuates Depression-Related Symptoms in Mice with Moderate Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Alessia Filippone,Laura Cucinotta,Valentina Bova,Marika Lanza,Giovanna Casili,Irene Paterniti,Michela Campolo,Salvatore Cuzzocrea,Emanuela Esposito

International journal of molecular sciences 24: PubMed36675183

2023

ATF3/SPI1/SLC31A1 Signaling Promotes Cuproptosis Induced by Advanced Glycosylation End Products in Diabetic Myocardial Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shengqi Huo,Qian Wang,Wei Shi,Lulu Peng,Yue Jiang,Mengying Zhu,Junyi Guo,Dewei Peng,Moran Wang,Lintong Men,Bingyu Huang,Jiagao Lv,Li Lin
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Product promise

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