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AB315367

Cortical Glutamatergic Neurons - Human iPSC derived cells (GFP - expressing) (Male, WC-30)

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These products are extremely sensitive and require specific conditions to maintain cell health and functionality. Please refer to the recommended protocols for the correct methodology in plating and maintaining these cells.
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Functional Studies - Cortical Glutamatergic Neurons - Human iPSC derived cells (GFP - expressing) (Male, WC-30) (AB315367)
  • FuncS

Supplier Data

Functional Studies - Cortical Glutamatergic Neurons - Human iPSC derived cells (GFP - expressing) (Male, WC-30) (AB315367)

This image was produced using ab315366 - the non GFP-expressing cells. Function : Cortical Glutamatergic Neurons (ab315366) exhibit pronounced electrophysiological activity after two weeks in culture, as demonstrated by multi-electrode array (MEA) recordings.

Immunocytochemistry/ Immunofluorescence - Cortical Glutamatergic Neurons - Human iPSC derived cells (GFP - expressing) (Male, WC-30) (AB315367)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Cortical Glutamatergic Neurons - Human iPSC derived cells (GFP - expressing) (Male, WC-30) (AB315367)

This image was produced using ab315366 - the non GFP-expressing cells.

Marker Expression : Cortical Glutamatergic Neurons (ab315366) have high neuronal purity (>90%) and comprise predominantly excitatory neurons. Labeling with the pan-neuronal marker MAP2 (red) highlights the neuronal purity of these cells. Labeling with the glutamatergic-specific markers CTIP2 (green) and TBR1 (blue) indicates overwhelming presence of glutamatergic neurons.

Immunocytochemistry/ Immunofluorescence - Cortical Glutamatergic Neurons - Human iPSC derived cells (GFP - expressing) (Male, WC-30) (AB315367)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Cortical Glutamatergic Neurons - Human iPSC derived cells (GFP - expressing) (Male, WC-30) (AB315367)

This image was produced using ab315366 - the non GFP-expressing cells. Morphology : Cortical Glutamatergic Neurons (ab315366) exhibit substantial neurite outgrowth within a week in culture and are adherent. Calcein staining (green) demonstrates the characteristic oval cell shape and long processes of Cortical Glutamatergic Neurons in culture.

Key facts

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Reactivity data

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Product details

These products are extremely sensitive and require specific conditions to maintain cell health and functionality. Please refer to the recommended protocols for the correct methodology in plating and maintaining these cells.

This product is a GFP expressing alternative to (ab315366).

Human Cortical Glutamatergic Neurons are derived from normal control iPSC line WC-30, ubiquitously expressing eGFP. BrainXell Cortical Glutamatergic Neurons have high neuronal purity (>80%) and comprise predominantly excitatory neurons. Labelling with the pan-neuronal marker MAP2 highlights the neuronal purity of these cells. Labelling with the glutamatergic-specific marker FOXP2 indicates overwhelming presence of glutamatergic neurons. Cortical Glutamatergic Neurons are adherent and exhibit substantial neurite outgrowth within the first week in culture, showing the characteristic oval cell shape and long processes of classic cortical glutamatergic neurons. These neurons also demonstrate pronounced electrophysiological activity after two weeks in culture, as demonstrated by multi-electrode array (MEA) recordings.

Vials of Cortical Glutamatergic Neurons are delivered with three supplements which are designed to facilitate the maturation and health of the cultures. BrainFast Glut is a seeding supplement which promotes cell survival at thaw and completes the differentiation process from late-stage neural progenitor to neuron. BrainFast SK enhances neuronal purity, and BrainFast D4 accelerates maturation.

In partnership with BrainXell

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What's included?

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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

See full target information Human Cortical Glutamatergic Neurons (GFP-Expressing) (Male, WC-30)
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websiteProtocolBooklet
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com