Creatine Assay Kit (Colorimetric/Fluorometric)

Creatine Assay Kit (Colorimetric/Fluorometric) (ab65339) provides an accurate, convenient measure of creatine in a variety of biological samples.

In the creatine assay protocol, creatine is enzymatically converted to sarcosine which is then specifically oxidized to generate a product that converts a colorless probe to an intensely red color (λmax = 570 nm), and highly fluorescent (Ex/Em = 538/587 nm) product. Creatine is therefore easily detected by either colorimetric or fluorometric methods. Detection range 0.001–10 mM Creatine.

Creatine assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 60 min
- analyze with a microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K635 Creatine Colorimetric/Fluorometric Assay Kit. K635-100 is the same size as the 100 test size of ab65339.

Creatine is an endogenous compound whose function is to maintain a high ATP/ADP ratio, by way of its phosphorylated form and creatine kinase. Creatine supplementation has been used in the treatment of muscular, neurological and neurodegenerative diseases, as well as a sport performance enhancer. Detection of creatine level has wide applications in research and development.