Creatine Kinase Activity Assay Kit (Colorimetric) ab155901 is a simple and fast assay (1 h assay time) for creatine kinase (CK) activity. Readout on any colorimetric (450 nm) plate reader.
- Kit includes standard curve for quantitation, and positive control enzyme
- Cited in > 50 publications.
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- PloS one 18:e02921242023Cinnamaldehyde alleviates doxorubicin-induced cardiotoxicity by decreasing oxidative stress and ferroptosis in cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeijiao Mao,Wang Zheng,Bin Deng,Youhua Wang,Duan Zhou,Lin Shen,Wankang Niku,Na ZhangPubMed 37824478
- JCI insight 8:2023Cytokine storm-based mechanisms for extrapulmonary manifestations of SARS-CoV-2 infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Del Nogal Avila,Ranjan Das,Joubert Kharlyngdoh,Eduardo Molina-Jijon,Hector Donoro Blazquez,Stéphanie Gambut,Michael Crowley,David K Crossman,Rasheed A Gbadegesin,Sunveer S Chugh,Sunjeet S Chugh,Carmen Avila-Casado,Camille Macé,Lionel C Clement,Sumant S ChughPubMed 37040185
- Bone & joint research 12:212-2182023Systemic glucose-insulin-potassium reduces skeletal muscle injury, kidney injury, and pain in a murine ischaemia-reperfusion model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel B Buchalter,David J Kirby,Utkarsh Anil,Sanjit R Konda,Philipp LeuchtPubMed 37051831
- Cell death & disease 13:10152022Valosin-containing protein (VCP/p97) inhibition reduces viral clearance and induces toxicity associated with muscular damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarta Del Rio Oliva,Michael BaslerPubMed 36456548
- JCI insight 7:2022NR1D1 controls skeletal muscle calcium homeostasis through myoregulin repression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexis Boulinguiez,Christian Duhem,Alicia Mayeuf-Louchart,Benoit Pourcet,Yasmine Sebti,Kateryna Kondratska,Valérie Montel,Stéphane Delhaye,Quentin Thorel,Justine Beauchamp,Aurore Hebras,Marion Gimenez,Marie Couvelaere,Mathilde Zecchin,Lise Ferri,Natalia Prevarskaya,Anne Forand,Christel Gentil,Jessica Ohana,France Piétri-Rouxel,Bruno Bastide,Bart Staels,Helene Duez,Steve LancelPubMed 35917173
- Nucleic acids research 50:11401-114142022Enhanced exon skipping and prolonged dystrophin restoration achieved by TfR1-targeted delivery of antisense oligonucleotide using FORCE conjugation in mdx mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCody A Desjardins,Monica Yao,John Hall,Emma O'Donnell,Reshmii Venkatesan,Sean Spring,Aiyun Wen,Nelson Hsia,Peiyi Shen,Ryan Russo,Bo Lan,Tyler Picariello,Kim Tang,Timothy Weeden,Stefano Zanotti,Romesh Subramanian,Oxana Ibraghimov-BeskrovnayaPubMed 35944903
- Current research in toxicology 3:1000832022Oxidative damage in metal fragment-embedded Sprague-Dawley rat gastrocnemius muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn F Kalinich,Vernieda B Vergara,Jessica F HoffmanPubMed 35935915
- Journal of cardiovascular development and disease 9:2022β-Caryophyllene Ameliorates Cyclophosphamide Induced Cardiac Injury: The Association of TLR4/NFκB and Nrf2/HO1/NQO1 Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNancy S YounisPubMed 35621844
- Cell death discovery 8:1782022HIF-1α-regulated lncRNA-TUG1 promotes mitochondrial dysfunction and pyroptosis by directly binding to FUS in myocardial infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong-Wang Wang,Hong-Zhi Dong,Yong-Xing Tan,Xu Bao,Ying-Man Su,Xin Li,Fang Jiang,Jing Liang,Zhen-Cai Huang,Yan-Ling Ren,Yu-Li Xu,Qiang SuPubMed 35396503
- Molecular medicine reports 25:2022Synergetic protective effect of remote ischemic preconditioning and prolyl 4‑hydroxylase inhibition in ischemic cardiac injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuesheng Yang,Jiajun Xu,Lei Tao,Shiyong Wang,Haiyan Xiang,Yanhua TangPubMed 35029283
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Tissue, Suspension cells, Tissue Homogenate, Serum, Adherent cells
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Range
- 2 - 10 nmol/well
- Assay time
- 1h
- Sensitivity
- < 1 mU/well
What's included?
Recommended products
Creatine Kinase Activity Assay Kit (Colorimetric) ab155901 is a simple and fast assay (1 h assay time) for creatine kinase (CK) activity. Readout on any colorimetric (450 nm) plate reader.
- Kit includes standard curve for quantitation, and positive control enzyme
- Cited in > 50 publications.
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Tissue, Suspension cells, Tissue Homogenate, Serum, Adherent cells
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Range
- 2 - 10 nmol/well
- Assay time
- 1h
- Assay Platform
- Microplate reader
- Sensitivity
- < 1 mU/well
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
How the assay works
In the creatine kinase assay protocol, creatine kinase (CK) converts creatine into phosphocreatine and ADP. The phosphocreatine and ADP then react with the CK enzyme mix to form an intermediate, which reduces a colorless probe to a colored product with strong absorbance at λ= 450 nm.
Creatine kinase assay protocol summary:
- Add samples and standards to wells
- Add reaction mix
- Analyze every 1-2 min for 10-40 min with microplate reader in kinetic mode at 37°C
How other researchers are using
Creatine Kinase Activity Assay Kit (Colorimetric) has been used in a variety of sample type including:
Human embryonic cardiomyocyte cell line H9C2 1
Mouse and rat glomerular tissues 2
Mouse skeletal muscle samples 3
References:
1-Mao M et al. 2023
2-Del Nogal Avila M et al. 2022
3- Buchalter D et al. 2023
This product is manufactured by BioVision, an Abcam company and was previously called K777 Creatine Kinase (CK) Activity Colorimetric Assay Kit. K777-100 is the same size as the 100 test size of ab155901.
Creatine Kinase (CK) also known as creatine phosphokinase (CPK) and ATP: creatine N-phosphotransferase is a common cellular enzyme (EC 2.7.3.2). It catalyzes the reversible conversion of creatine and ATP into ADP and phosphocreatine.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901)
Figure 1.
Creatine kinase activity tested in 5 μl human serum and 192 ng rat heart lysate. Assay performed following kit protocol.
Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901)
Creatine Kinase Activity measured in various samples.
Initial protein concentration for tissue lysates varied from 1 mg/mL to 3 mg/mL. 40 μl of filtered tissue lysates were used. 30 μl of filtered biological fluids were used.
Sengle, Gerhard et al., PLoS genetics: vol. 11,6 e1005340., Table 1, doi:10.1371/journal.pgen.1005340Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901)
Creatine kinase activities in blood samples from wildtype and Fbn2 null mice.
Mean +/- standard deviation for a given genotype and age is shown, except where only one animal was tested. N = number of animals tested. Units are nmol NADH/min/ml.
Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901)
Functional studies - Creatine Kinase Activity Assay Kit.
Typical NADH standard calibration curve using colorimetric reading.
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