Creatine Kinase Activity Assay Kit (Colorimetric)
4
(2 Reviews)
|
(60 Publications)
- Kit includes standard curve for quantitation, and positive control enzyme
- Cited in > 50 publications.
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
- FuncS
Supplier Data
Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901)
Creatine kinase activity tested in 5 μl human serum and 192 ng rat heart lysate. Assay performed following kit protocol.
- FuncS
Unknown
Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901)
Creatine kinase activities in blood samples from wildtype and Fbn2 null mice.
Mean +/- standard deviation for a given genotype and age is shown, except where only one animal was tested. N = number of animals tested. Units are nmol NADH/min/ml.
Sengle, Gerhard et al., PLoS genetics: vol. 11,6 e1005340., Table 1, doi:10.1371/journal.pgen.1005340
- FuncS
Supplier Data
Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901)
Functional studies - Creatine Kinase Activity Assay Kit.
Typical NADH standard calibration curve using colorimetric reading.
- FuncS
Lab
Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901)
Creatine Kinase Activity measured in various samples.
Initial protein concentration for tissue lysates varied from 1 mg/mL to 3 mg/mL. 40 μl of filtered tissue lysates were used. 30 μl of filtered biological fluids were used.
- Schematic Diagram
Supplier Data
Schematic Diagram - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901)
Representative image of Creatine Kinase Activity Assay Kit (Colorimetric) ab155901
Components shown from left to right :
- NADH Standard I
- Creatine
- Developer Solution III
- Active Creatine Kinase
- ATP I
- CK Enzyme Mix
- Assay Buffer 20
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.
Reactivity data
Product details
How the assay works
In the creatine kinase assay protocol, creatine kinase (CK) converts creatine into phosphocreatine and ADP. The phosphocreatine and ADP then react with the CK enzyme mix to form an intermediate, which reduces a colorless probe to a colored product with strong absorbance at λ= 450 nm.
Creatine kinase assay protocol summary:
- Add samples and standards to wells
- Add reaction mix
- Analyze every 1-2 min for 10-40 min with microplate reader in kinetic mode at 37°C
How other researchers are using
Creatine Kinase Activity Assay Kit (Colorimetric) has been used in a variety of sample type including:
Human embryonic cardiomyocyte cell line H9C2 1
Mouse and rat glomerular tissues 2
Mouse skeletal muscle samples 3
References:
1-Mao M et al. 2023
2-Del Nogal Avila M et al. 2022
3- Buchalter D et al. 2023
This product is manufactured by BioVision, an Abcam company and was previously called K777 Creatine Kinase (CK) Activity Colorimetric Assay Kit. K777-100 is the same size as the 100 test size of ab155901.
Creatine Kinase (CK) also known as creatine phosphokinase (CPK) and ATP: creatine N-phosphotransferase is a common cellular enzyme (EC 2.7.3.2). It catalyzes the reversible conversion of creatine and ATP into ADP and phosphocreatine.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (60)
Recent publications for all applications. Explore the full list and refine your search
Nature communications 16:2398 PubMed40064877
2025
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PloS one 19:e0313693 PubMed39621655
2024
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Antioxidants (Basel, Switzerland) 13: PubMed39594567
2024
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Redox biology 78:103418 PubMed39531828
2024
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eLife 13: PubMed39316042
2024
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General physiology and biophysics 43:13-23 PubMed38312031
2024
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Open veterinary journal 13:1718-1728 PubMed38292716
2024
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PloS one 18:e0292124 PubMed37824478
2023
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Journal of controlled release : official journal of the Controlled Release Society 362:536-547 PubMed37648082
2023
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JCI insight 8: PubMed37040185
2023
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com