Creatine Kinase Activity Assay Kit (Colorimetric) ab155901 uses a high-throughput adaptable, simple and sensitive assay for creatine kinase (CK) activity.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Colorimetric
Plasma, Tissue, Suspension cells, Tissue Homogenate, Serum, Adherent cells
Enzyme activity
Mammals
2 - 10 nmol/well
1h
< 1 mU/well
Creatine Kinase Activity Assay Kit (Colorimetric) ab155901 uses a high-throughput adaptable, simple and sensitive assay for creatine kinase (CK) activity.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Colorimetric
Plasma, Tissue, Suspension cells, Tissue Homogenate, Serum, Adherent cells
Enzyme activity
Mammals
2 - 10 nmol/well
1h
Microplate reader
< 1 mU/well
Blue Ice
-20°C
-20°C
-20°C
Creatine Kinase Activity Assay Kit (Colorimetric) ab155901 uses a high-throughput adaptable, simple and sensitive assay for creatine kinase (CK) activity.
In the creatine kinase assay protocol, creatine kinase (CK) converts creatine into phosphocreatine and ADP. The phosphocreatine and ADP then react with the CK enzyme mix to form an intermediate, which reduces a colorless probe to a colored product with strong absorbance at λ= 450 nm.
This assay kit can detect CK activity to less than 1 mU.
Creatine kinase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze every 1-2 min for 10-40 min with microplate reader in kinetic mode at 37°C
This product is manufactured by BioVision, an Abcam company and was previously called K777 Creatine Kinase (CK) Activity Colorimetric Assay Kit. K777-100 is the same size as the 100 test size of ab155901.
Creatine Kinase (CK) also known as creatine phosphokinase (CPK) and ATP: creatine N-phosphotransferase is a common cellular enzyme (EC 2.7.3.2). It catalyzes the reversible conversion of creatine and ATP into ADP and phosphocreatine.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Creatine Kinase Activity measured in various samples.
Initial protein concentration for tissue lysates varied from 1 mg/mL to 3 mg/mL. 40 μl of filtered tissue lysates were used. 30 μl of filtered biological fluids were used.
Creatine kinase activities in blood samples from wildtype and Fbn2 null mice.
Mean +/- standard deviation for a given genotype and age is shown, except where only one animal was tested. N = number of animals tested. Units are nmol NADH/min/ml.
Functional studies - Creatine Kinase Activity Assay Kit.
Typical NADH standard calibration curve using colorimetric reading.
Figure 1.
Creatine kinase activity tested in 5 μl human serum and 192 ng rat heart lysate. Assay performed following kit protocol.
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