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Creatine Kinase Activity Assay Kit (Colorimetric) ab155901 uses a high-throughput adaptable, simple and sensitive assay for creatine kinase (CK) activity.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.


Images

Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901), expandable thumbnail
  • Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901), expandable thumbnail
  • Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901), expandable thumbnail
  • Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue, Suspension cells, Tissue Homogenate, Serum, Adherent cells

Assay type

Enzyme activity

Reactive species

Mammals

Range

2 - 10 nmol/well

Assay time

1h

Sensitivity

< 1 mU/well

What's included?

100 Test
Components
ATP I
1 x 1 Vial
Active Creatine Kinase
1 x 1 Vial
Assay Buffer XX
1 x 25 mL
CK Enzyme Mix
1 x 1 Vial
Creatine
1 x 1 mL
Developer Solution III
1 x 1 Vial
NADH Standard I
1 x 1 Vial

Recommended products

Creatine Kinase Activity Assay Kit (Colorimetric) ab155901 uses a high-throughput adaptable, simple and sensitive assay for creatine kinase (CK) activity.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue, Suspension cells, Tissue Homogenate, Serum, Adherent cells

Assay type

Enzyme activity

Reactive species

Mammals

Range

2 - 10 nmol/well

Assay time

1h

Assay Platform

Microplate reader

Sensitivity

< 1 mU/well

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Creatine Kinase Activity Assay Kit (Colorimetric) ab155901 uses a high-throughput adaptable, simple and sensitive assay for creatine kinase (CK) activity.

In the creatine kinase assay protocol, creatine kinase (CK) converts creatine into phosphocreatine and ADP. The phosphocreatine and ADP then react with the CK enzyme mix to form an intermediate, which reduces a colorless probe to a colored product with strong absorbance at λ= 450 nm.

This assay kit can detect CK activity to less than 1 mU.

Creatine kinase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze every 1-2 min for 10-40 min with microplate reader in kinetic mode at 37°C

This product is manufactured by BioVision, an Abcam company and was previously called K777 Creatine Kinase (CK) Activity Colorimetric Assay Kit. K777-100 is the same size as the 100 test size of ab155901.

Creatine Kinase (CK) also known as creatine phosphokinase (CPK) and ATP: creatine N-phosphotransferase is a common cellular enzyme (EC 2.7.3.2). It catalyzes the reversible conversion of creatine and ATP into ADP and phosphocreatine.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901), expandable thumbnail

    Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901)

    Creatine Kinase Activity measured in various samples.

    Initial protein concentration for tissue lysates varied from 1 mg/mL to 3 mg/mL. 40 μl of filtered tissue lysates were used. 30 μl of filtered biological fluids were used.

  • Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901), expandable thumbnail
    Sengle, Gerhard et al., PLoS genetics?vol. 11,6 e1005340., Table 1, doi:10.1371/journal.pgen.1005340

    Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901)

    Creatine kinase activities in blood samples from wildtype and Fbn2 null mice.
    Mean +/- standard deviation for a given genotype and age is shown, except where only one animal was tested. N = number of animals tested. Units are nmol NADH/min/ml.

  • Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901), expandable thumbnail

    Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901)

    Functional studies - Creatine Kinase Activity Assay Kit.

    Typical NADH standard calibration curve using colorimetric reading.

  • Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901), expandable thumbnail

    Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901)

    Figure 1.

    Creatine kinase activity tested in 5 μl human serum and 192 ng rat heart lysate. Assay performed following kit protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com