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AB155901

Creatine Kinase Activity Assay Kit (Colorimetric)

4

(2 Reviews)

|

(60 Publications)

Creatine Kinase Activity Assay Kit (Colorimetric) ab155901 is a simple and fast assay (1 h assay time) for creatine kinase (CK) activity. Readout on any colorimetric (450 nm) plate reader.

- Kit includes standard curve for quantitation, and positive control enzyme
- Cited in > 50 publications.
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
5 Images
Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901)
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Supplier Data

Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901)

Creatine kinase activity tested in 5 μl human serum and 192 ng rat heart lysate. Assay performed following kit protocol.

Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901)
  • FuncS

Unknown

Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901)

Creatine kinase activities in blood samples from wildtype and Fbn2 null mice.
Mean +/- standard deviation for a given genotype and age is shown, except where only one animal was tested. N = number of animals tested. Units are nmol NADH/min/ml.

Sengle, Gerhard et al., PLoS genetics: vol. 11,6 e1005340., Table 1, doi:10.1371/journal.pgen.1005340

Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901)
  • FuncS

Supplier Data

Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901)

Functional studies - Creatine Kinase Activity Assay Kit.

Typical NADH standard calibration curve using colorimetric reading.

Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901)
  • FuncS

Lab

Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901)

Creatine Kinase Activity measured in various samples.

Initial protein concentration for tissue lysates varied from 1 mg/mL to 3 mg/mL. 40 μl of filtered tissue lysates were used. 30 μl of filtered biological fluids were used.

Schematic Diagram - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901)

Representative image of Creatine Kinase Activity Assay Kit (Colorimetric) ab155901

Components shown from left to right :

- NADH Standard I

- Creatine

- Developer Solution III

- Active Creatine Kinase

- ATP I

- CK Enzyme Mix

- Assay Buffer 20

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue, Suspension cells, Tissue Homogenate, Serum, Adherent cells

Assay type

Enzyme activity

Sensitivity

< 1 mU/well

Range

2 - 10 nmol/well

Assay time

1h

Assay Platform

Microplate reader

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

How the assay works

In the creatine kinase assay protocol, creatine kinase (CK) converts creatine into phosphocreatine and ADP. The phosphocreatine and ADP then react with the CK enzyme mix to form an intermediate, which reduces a colorless probe to a colored product with strong absorbance at λ= 450 nm.

Creatine kinase assay protocol summary:

- Add samples and standards to wells
- Add reaction mix
- Analyze every 1-2 min for 10-40 min with microplate reader in kinetic mode at 37°C

How other researchers are using

Creatine Kinase Activity Assay Kit (Colorimetric) has been used in a variety of sample type including:
Human embryonic cardiomyocyte cell line H9C2 1
Mouse and rat glomerular tissues 2
Mouse skeletal muscle samples 3
References:

1-Mao M et al. 2023
2-Del Nogal Avila M et al. 2022
3- Buchalter D et al. 2023

This product is manufactured by BioVision, an Abcam company and was previously called K777 Creatine Kinase (CK) Activity Colorimetric Assay Kit. K777-100 is the same size as the 100 test size of ab155901.

Creatine Kinase (CK) also known as creatine phosphokinase (CPK) and ATP: creatine N-phosphotransferase is a common cellular enzyme (EC 2.7.3.2). It catalyzes the reversible conversion of creatine and ATP into ADP and phosphocreatine.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab155901-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"NADH Standard I", "number":"AB155901-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution III", "number":"AB155901-CMP03", "productcode":"AB255098" }, { "size":"1 x 1 mL", "name":"Creatine", "number":"AB155901-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"CK Enzyme Mix", "number":"AB155901-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 20", "number":"AB155901-CMP02", "productcode":"AB323962" }, { "size":"1 x 1 Vial", "name":"Active Creatine Kinase", "number":"AB155901-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"ATP I", "number":"AB155901-CMP01", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Publications (60)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2398 PubMed40064877

2025

Activation of endogenous full-length utrophin by MyoAAV-UA as a therapeutic approach for Duchenne muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Ruo Wu,Peng Li,Puhao Xiao,Shu Zhang,Xiaopeng Wang,Jie Liu,Wenjie Sun,Yue Chang,Xiuyi Ai,Lijiao Chen,Yan Zhuo,Jiaojian Wang,Zhengbo Wang,Shangang Li,Yuanyuan Li,Weizhi Ji,Wenting Guo,Shiwen Wu,Yongchang Chen

PloS one 19:e0313693 PubMed39621655

2024

Anti-necroptotic effects of human Wharton's jelly-derived mesenchymal stem cells in skeletal muscle cell death model via secretion of GRO-α.

Applications

Unspecified application

Species

Unspecified reactive species

Sang Eon Park,Soo Jin Kwon,Sun Jeong Kim,Jang Bin Jeong,Min-Jeong Kim,Suk-Joo Choi,Soo-Young Oh,Gyu Ha Ryu,Hong Bae Jeon,Jong Wook Chang

Antioxidants (Basel, Switzerland) 13: PubMed39594567

2024

25-Hydroxycholecalciferol Improves Cardiac Metabolic Adaption, Mitochondrial Biogenetics, and Redox Status to Ameliorate Pathological Remodeling and Functional Failure in Obese Chickens.

Applications

Unspecified application

Species

Unspecified reactive species

Shih-Kai Chiang,Mei-Ying Sin,Jun-Wen Lin,Maraddin Siregar,Gilmour Valdez,Yu-Hui Chen,Thau Kiong Chung,Rosemary L Walzem,Lin-Chu Chang,Shuen-Ei Chen

Redox biology 78:103418 PubMed39531828

2024

Deficiency of muscle-generated brain-derived neurotrophic factor causes inflammatory myopathy through reactive oxygen species-mediated necroptosis and pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Brian Pak Shing Pang,Elsie Chit Yu Iu,Miaojia Hang,Wing Suen Chan,Margaret Chui Ling Tse,Connie Tsz Ying Yeung,Mingfu Wang,Parco Ming Fai Siu,Chi Wai Lee,Keqiang Ye,Ho So,Chi Bun Chan

eLife 13: PubMed39316042

2024

Identification of pharmacological inducers of a reversible hypometabolic state for whole organ preservation.

Applications

Unspecified application

Species

Unspecified reactive species

Megan M Sperry,Berenice Charrez,Haleh Fotowat,Erica Gardner,Kanoelani Pilobello,Zohreh Izadifar,Tiffany Lin,Abigail Kuelker,Sahith Kaki,Michael Lewandowski,Shanda Lightbown,Ramses Martinez,Susan Marquez,Joel Moore,Maria Plaza-Oliver,Adama M Sesay,Kostyantyn Shcherbina,Katherine Sheehan,Takako Takeda,Daniela Del Campo,Kristina Andrijauskaite,Exal Cisneros,Riley Lopez,Isabella Cano,Zachary Maxwell,Israel Jessop,Rafa Veraza,Leon Bunegin,Thomas J Percival,Jaclyn Yracheta,Jorge J Pena,Diandra M Wood,Zachary T Homas,Cody J Hinshaw,Jennifer Cox-Hinshaw,Olivia G Parry,Justin J Sleeter,Erik K Weitzel,Michael Levin,Michael Super,Richard Novak,Donald E Ingber

General physiology and biophysics 43:13-23 PubMed38312031

2024

Naringenin protects myocardial ischemia/reperfusion injury by regulating miR-24-3p to inhibit cell death-inducing p53 target 1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Jin,Ling Jin,Bingxin Wu,Danping Xu

Open veterinary journal 13:1718-1728 PubMed38292716

2024

The role of hesperidin as a cardioprotective strategy against doxorubicin-induced cardiotoxicity: The antioxidant, anti-inflammatory, antiapoptotic, and cytoprotective potentials.

Applications

Unspecified application

Species

Unspecified reactive species

Fawiziah Khalaf Alharbi,Zafer S Alshehri,Faez F Alshehri,Sharif Alhajlah,Hesham A Khalifa,Naief Dahran,Wael A M Ghonimi

PloS one 18:e0292124 PubMed37824478

2023

Cinnamaldehyde alleviates doxorubicin-induced cardiotoxicity by decreasing oxidative stress and ferroptosis in cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Meijiao Mao,Wang Zheng,Bin Deng,Youhua Wang,Duan Zhou,Lin Shen,Wankang Niku,Na Zhang

Journal of controlled release : official journal of the Controlled Release Society 362:536-547 PubMed37648082

2023

Peptide delivery of a multivalent mRNA SARS-CoV-2 vaccine.

Applications

Unspecified application

Species

Unspecified reactive species

Cian M McCrudden,Lindsey Bennie,Philip Chambers,Jordan Wilson,Megan Kerr,Monika Ziminska,Hayley Douglas,Sarah Kuhn,Emma Carroll,Garrett O'Brien,Niamh Buckley,Nicholas J Dunne,Helen O McCarthy

JCI insight 8: PubMed37040185

2023

Cytokine storm-based mechanisms for extrapulmonary manifestations of SARS-CoV-2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Del Nogal Avila,Ranjan Das,Joubert Kharlyngdoh,Eduardo Molina-Jijon,Hector Donoro Blazquez,Stéphanie Gambut,Michael Crowley,David K Crossman,Rasheed A Gbadegesin,Sunveer S Chugh,Sunjeet S Chugh,Carmen Avila-Casado,Camille Macé,Lionel C Clement,Sumant S Chugh
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