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Creatine Kinase Activity Assay Kit (Colorimetric) ab155901 is a simple and fast assay (1 h assay time) for creatine kinase (CK) activity. Readout on any colorimetric (450 nm) plate reader.

- Kit includes standard curve for quantitation, and positive control enzyme
- Cited in > 50 publications.
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.


Images

Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901), expandable thumbnail
  • Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901), expandable thumbnail
  • Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901), expandable thumbnail
  • Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (AB155901), expandable thumbnail

Publications

Key facts

Detection method
Colorimetric
Sample types
Plasma, Tissue, Suspension cells, Tissue Homogenate, Serum, Adherent cells
Assay type
Enzyme activity
Reactive species
Mammals
Range
2 - 10 nmol/well
Assay time
1h
Sensitivity
< 1 mU/well

What's included?

100 Test
Components
ATP I
1 x 1 Each
Active Creatine Kinase
1 x 1 Each
Assay Buffer 20
1 x 25 mL
CK Enzyme Mix
1 x 1 Each
Creatine
1 x 1 mL
Developer Solution III
1 x 1 Each
NADH Standard I
1 x 1 Vial

Recommended products

Creatine Kinase Activity Assay Kit (Colorimetric) ab155901 is a simple and fast assay (1 h assay time) for creatine kinase (CK) activity. Readout on any colorimetric (450 nm) plate reader.

- Kit includes standard curve for quantitation, and positive control enzyme
- Cited in > 50 publications.
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Key facts

Detection method
Colorimetric
Sample types
Plasma, Tissue, Suspension cells, Tissue Homogenate, Serum, Adherent cells
Assay type
Enzyme activity
Reactive species
Mammals
Range
2 - 10 nmol/well
Assay time
1h
Assay Platform
Microplate reader
Sensitivity
< 1 mU/well

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

How the assay works

In the creatine kinase assay protocol, creatine kinase (CK) converts creatine into phosphocreatine and ADP. The phosphocreatine and ADP then react with the CK enzyme mix to form an intermediate, which reduces a colorless probe to a colored product with strong absorbance at λ= 450 nm.

Creatine kinase assay protocol summary:

- Add samples and standards to wells
- Add reaction mix
- Analyze every 1-2 min for 10-40 min with microplate reader in kinetic mode at 37°C

How other researchers are using

Creatine Kinase Activity Assay Kit (Colorimetric) has been used in a variety of sample type including:
Human embryonic cardiomyocyte cell line H9C2 1
Mouse and rat glomerular tissues 2
Mouse skeletal muscle samples 3
References: 

1-Mao M et al. 2023
2-Del Nogal Avila M et al. 2022
3- Buchalter D et al. 2023

This product is manufactured by BioVision, an Abcam company and was previously called K777 Creatine Kinase (CK) Activity Colorimetric Assay Kit. K777-100 is the same size as the 100 test size of ab155901.

Creatine Kinase (CK) also known as creatine phosphokinase (CPK) and ATP: creatine N-phosphotransferase is a common cellular enzyme (EC 2.7.3.2). It catalyzes the reversible conversion of creatine and ATP into ADP and phosphocreatine.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901), expandable thumbnail

    Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901)

    Figure 1.

    Creatine kinase activity tested in 5 μl human serum and 192 ng rat heart lysate. Assay performed following kit protocol.

  • Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901), expandable thumbnail

    Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901)

    Creatine Kinase Activity measured in various samples.

    Initial protein concentration for tissue lysates varied from 1 mg/mL to 3 mg/mL. 40 μl of filtered tissue lysates were used. 30 μl of filtered biological fluids were used.

  • Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901), expandable thumbnail
    Sengle, Gerhard et al., PLoS genetics: vol. 11,6 e1005340., Table 1, doi:10.1371/journal.pgen.1005340

    Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901)

    Creatine kinase activities in blood samples from wildtype and Fbn2 null mice.
    Mean +/- standard deviation for a given genotype and age is shown, except where only one animal was tested. N = number of animals tested. Units are nmol NADH/min/ml.

  • Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901), expandable thumbnail

    Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901)

    Functional studies - Creatine Kinase Activity Assay Kit.

    Typical NADH standard calibration curve using colorimetric reading.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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