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Creatinine Assay Kit ab65340 provides an accurate, convenient measure of creatinine concentration in biological fluids such as serum, urine or CSF.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.


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Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Cerebral Spinal Fluid, Other biological fluids, Serum, Urine

Assay type

Quantitative

Assay time

1h

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What's included?

2000 Test
Components
Assay Buffer II
20 x 25 mL
Creatinase
20 x 1 Vial
Creatininase
20 x 1 Vial
Creatinine Standard
20 x 1 Vial
OxiRed Probe
20 x 200 µL
Sarcosine Enzyme Mix
20 x 1 Vial

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Creatinine Assay Kit ab65340 provides an accurate, convenient measure of creatinine concentration in biological fluids such as serum, urine or CSF.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Cerebral Spinal Fluid, Other biological fluids, Serum, Urine

Assay type

Quantitative

Assay time

1h

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Creatinine Assay Kit ab65340 provides an accurate, convenient measure of creatinine concentration in biological fluids such as serum, urine or CSF.

In the creatinine assay protocol, creatinine is converted to creatine by creatininase, creatine is converted to sarcosine, which is specifically oxidized to produce a product which reacts with a probe to generate red color (λmax = 570 nm) and fluorescence (Ex/Em = 538/587 nm).

Unlike picric acid assays, this kit is suitable for serum creatinine determinations, as well as for urine and other biological samples.

Creatinine assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 60 min at 37°C
- analyze with microplate reader

For deproteinization of samples: Better results are typically seen with this assay when using a 10kda filter for sample deproteinization than when using the PCA method.

This product is manufactured by BioVision, an Abcam company and was previously called K625 Creatinine Colorimetric/Fluorometric Assay Kit. K625-100 is the same size as the 100 test size of ab65340.

Supplementary info

Biological function summary

In the human body creatinine plays an integral role in reflecting muscle metabolism rates and biochemical balance. Creatinine levels aid in assessing glomerular filtration rate (GFR) an important measure of kidney function. It is not part of a protein complex as it results from muscle activity. Its levels in blood and urine help calculate the protein to creatinine ratio providing insights into renal health. The constant exchange and excretion of creatinine make it a reliable biomarker in metabolism studies.

Activity summary

Creatinine also known as 2-amino-1-methyl-5H-imidazol-4-one is a small molecule with a molecular mass of approximately 113.12 g/mol. Its mechanical functions stem from its role as a breakdown product of creatine phosphate primarily in muscle. Creatinine serves as a marker for kidney function due to its constant production and relatively stable concentration in the blood. It gets filtered out of the blood by the kidneys and is present in urine. Laboratories focus on measuring creatinine levels using tools like the creatinine assay kit and creatinine test kit to evaluate renal function.

Pathways

Muscle metabolism and kidney filtration both involve creatinine's function. Creatinine formation and elimination connect to pathways that maintain homeostasis and waste removal. Key proteins in these pathways include creatine kinase which catalyzes the conversion of creatine to creatine phosphate. The creatinine protein ratio becomes relevant in evaluating proteinuria a condition where excess protein is present in urine indicating possible kidney dysfunction.

Associated diseases and disorders

Kidney-related conditions and muscle disorders often involve creatinine. Common health concerns like chronic kidney disease (CKD) associate with elevated creatinine levels indicating reduced kidney function. Additionally conditions such as muscular dystrophy can alter muscle creatinine levels. These diseases relate to proteins such as nephrin involved in the kidney filtration barrier and dystrophin essential for muscular integrity. For clinical assessments tools like the creatinine assay and understanding the protein creatinine ratio are key for early detection and management.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Functional Studies - Creatinine Assay Kit (ab65340), expandable thumbnail
    Gai, Zhibo et al., Scientific reports vol. 7,1 9815., Fig 2, doi:10.1038/s41598-017-10168-6

    Functional Studies - Creatinine Assay Kit (ab65340)

    Mice were injected i.p. with 6-ECDCA before I/R injury. At 24 h after I/R, kidney, serum and urine samples were collected for measurements of creatinine in serum with ab65340

  • Functional Studies - Creatinine Assay Kit (ab65340), expandable thumbnail

    Functional Studies - Creatinine Assay Kit (ab65340)

    Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Functional Studies - Creatinine Assay Kit (ab65340), expandable thumbnail

    Functional Studies - Creatinine Assay Kit (ab65340)

    Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Functional Studies - Creatinine Assay Kit (ab65340), expandable thumbnail

    Functional Studies - Creatinine Assay Kit (ab65340)

    Creatinine measured in mouse and human serum plotted against RFU. Samples were diluted 5-10 fold.

  • Functional Studies - Creatinine Assay Kit (ab65340), expandable thumbnail

    Functional Studies - Creatinine Assay Kit (ab65340)

    Creatinine measured in mouse and human urine plotted against RFU. Samples were diluted 400-800 fold.

  • Immunodiffusion - Creatinine Assay Kit (ab65340), expandable thumbnail

    Immunodiffusion - Creatinine Assay Kit (ab65340)

    Creatinine levels in filtered human urine was measured in the presence or absence of creatininase (background signal subtracted.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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