Creatinine Assay Kit ab65340 provides an accurate, convenient measure of creatinine concentration in biological fluids such as serum, urine or CSF.
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- Nature communications 15:3712024Deletion of Aurora kinase A prevents the development of polycystic kidney disease in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Shen Tham,Denny L Cottle,Allara K Zylberberg,Kieran M Short,Lynelle K Jones,Perkin Chan,Sarah E Conduit,Jennifer M Dyson,Christina A Mitchell,Ian M SmythPubMed 38191531
- Science advances 9:eadi47772023Adrenomedullin has a pivotal role in trophoblast differentiation: A promising nanotechnology-based therapeutic target for early-onset preeclampsia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingqing Zhang,Cheuk-Lun Lee,Tingyu Yang,Jianlin Li,Qunxiong Zeng,Xiaofeng Liu,Zhongzhen Liu,Degong Ruan,Zhuoxuan Li,Anita S Y Kan,Ka-Wang Cheung,Annisa S L Mak,Vivian W Y Ng,Huashan Zhao,Xiujun Fan,Yong-Gang Duan,Liuying Zhong,Min Chen,Meirong Du,Raymond H W Li,Pengtao Liu,Ernest H Y Ng,William S B Yeung,Ya Gao,Yuanqing Yao,Philip C N ChiuPubMed 37922358
- Lupus science & medicine 10:2023p16, a marker of cellular senescence, is associated with renal disease in the B6. mouse model of lupus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaëlle Tilman,Emilie Dupré,Laura Watteyne,Charlotte Anne Baert,Delphine Nolf,Fatima Benhaddi,Fanny Lambert,Aurélie Daumerie,Caroline Bouzin,Sophie Lucas,Nisha LimayePubMed 37899089
- Molecular medicine (Cambridge, Mass.) 29:1382023Knockout of STE20-type kinase TAOK3 does not attenuate diet-induced NAFLD development in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Xia,Emma Andersson,Mara Caputo,Emmelie Cansby,Francesca Sedda,Ferran Font-Gironès,Johan Ruud,Yeshwant Kurhe,Bengt Hallberg,Hanns-Ulrich Marschall,Ingrid Wernstedt Asterholm,Stefano Romeo,Matthias Blüher,Margit MahlapuuPubMed 37864157
- International journal of biological sciences 19:5145-51592023PTEN-induced kinase 1 exerts protective effects in diabetic kidney disease by attenuating mitochondrial dysfunction and necroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin-Ji Sung,Hyun-Ju An,Min Heui Ha,Seon Hwa Park,Hye Yun Jeong,Jihyun Baek,Sang Ho Lee,Yu Ho Lee,So-Young LeePubMed 37928264
- Molecular therapy. Nucleic acids 34:1020412023A mutual regulatory loop between miR-155 and SOCS1 influences renal inflammation and diabetic kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIgnacio Prieto,María Kavanagh,Luna Jimenez-Castilla,Marisa Pardines,Iolanda Lazaro,Isabel Herrero Del Real,Monica Flores-Muñoz,Jesus Egido,Oscar Lopez-Franco,Carmen Gomez-GuerreroPubMed 37842165
- Physiological reports 11:e158092023Investigation of mesalazine as an antifibrotic drug following myocardial infarction in male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephan R Künzel,Luise Winter,Maximilian Hoffmann,Theresa A Kant,Jessica Thiel,Romy Kronstein-Wiedemann,Erik Klapproth,Kristina Lorenz,Ali El-Armouche,Susanne KämmererPubMed 37688424
- Cellular and molecular biology (Noisy-le-Grand, France) 69:198-2022023Nuclear factor-kappa B p50-induced microRNA-20a-3p plays a detrimental role in sepsis-induced acute kidney injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeihong Mao,Xiaoming Wang,Yongchen Zhang,Huding Zhu,Li Dai,Juanjuan ChenPubMed 37715384
- PloS one 18:e02894762023The effects of a comparatively higher dose of 1000 mg/kg/d of oral L- or D-arginine on the L-arginine metabolic pathways in male Sprague-Dawley rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDain Raina Kim,Sarah Martin,Kaushik DesaiPubMed 37527267
- EBioMedicine 93:1046642023H3K27me3-modulated Hofbauer cell BMP2 signalling enhancement compensates for shallow trophoblast invasion in preeclampsia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianye Deng,Hong-Jin Zhao,Ying Zhong,Cuiping Hu,Jinlai Meng,Chunling Wang,Xiangxin Lan,Xiyao Wang,Zi-Jiang Chen,Junhao Yan,Wei Wang,Yan LiPubMed 37331163
Key facts
- Detection method
- Colorimetric/Fluorometric
- Sample types
- Other biological fluids, Serum, Urine
- Assay type
- Quantitative
- Assay time
- 1h
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Creatinine Assay Kit ab65340 provides an accurate, convenient measure of creatinine concentration in biological fluids such as serum, urine or CSF.
Key facts
- Detection method
- Colorimetric/Fluorometric
- Sample types
- Other biological fluids, Serum, Urine
- Assay type
- Quantitative
- Assay time
- 1h
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Creatinine Assay Kit ab65340 provides an accurate, convenient measure of creatinine concentration in biological fluids such as serum, urine or CSF.
How the assay works
In the creatinine assay protocol, creatinine is converted to creatine by creatininase, creatine is converted to sarcosine by creatinase. Sarcosine is then oxidized by sarcosine oxidase producing hydrogen peroxide. Hydrogen peroxide is then processed by a peroxidase causing a reaction with a probe to generate red color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm).
Creatinine assay protocol summary
- - Add samples and standards to wells.
- - Add reaction mix and incubate for 60 min at 37°C.
- - Analyze with microplate reader.
Getting the best performance from ab65340
Unlike picric acid assays, this kit is suitable for serum creatinine determinations, as well as for urine and other biological samples.
For deproteinization of samples: Better results are typically seen with this assay when using a 10kda filter for sample deproteinization than when using the PCA method.
Related and recommended products
Creatinine assay kit ab65340 is commonly used by researchers to measure kidney function.
Kidney function marker assays include:
- - Creatinine Assay Kit ab65340 - suitable for use as a serum creatinine assay and a urine creatinine assay.
- - Mouse Albumin ELISA Kit Mouse Albumin ELISA Kit ab108792 and Human Albumin ELISA Kit Human Albumin ELISA Kit ab227933 – suitable for use with serum/plasma and urine
- - Albumin (BCG) Assay Kit (Colorimetric) Albumin (BCG) Assay Kit (Colorimetric) ab235628 – suitable for use with serum
- - Urea Assay Kit Urea Assay Kit ab83362 – suitable for use with serum/plasma and urine, and used as a blood urea nitrogen assay.
The albumin-creatinine ratio is often measured by using in combination Creatine Assay Kit ab65340, and either Mouse Albumin ELISA Kit Mouse Albumin ELISA Kit ab108792 or Human Albumin ELISA Kit Human Albumin ELISA Kit ab227933.
The urea-creatinine ratio is often measured by using in combination Urea Assay Kit Urea Assay Kit ab83362 and Creatinine Assay Kit ab65340.
Albumin Creatinine Ratio Assay Kit Albumin Creatinine Ratio Assay Kit ab241018 is suitable for urine and serum, and combines a fluorescent albumin assay with a colorimetric creatinine assay in a convenient pack.
Creatinine assay methods
There are two established methods of creatinine assays:
- Jaffe assay
The Jaffe assay method is based on the reaction of creatinine with picric acid at an alkaline pH. The creatinine‐picrate complex has an absorbance of 500 nm.
- Enzymatic creatinine assay
The standard enzymatic creatinine assay uses the method described above for ab65340.
The Jaffe method is substantially simpler and cheaper than enzymatic creatinine assays, however it suffers substantially from interference by metabolites found in urine and serum.
Other notes
This product is manufactured by BioVision, an Abcam company and was previously called K625 Creatinine Colorimetric/Fluorometric Assay Kit. K625-100 is the same size as the 100 test size of ab65340.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Creatinine also known as 2-amino-1-methyl-5H-imidazol-4-one is a small molecule with a molecular mass of approximately 113.12 g/mol. Its mechanical functions stem from its role as a breakdown product of creatine phosphate primarily in muscle. Creatinine serves as a marker for kidney function due to its constant production and relatively stable concentration in the blood. It gets filtered out of the blood by the kidneys and is present in urine. Laboratories focus on measuring creatinine levels using tools like the creatinine assay kit and creatinine test kit to evaluate renal function.
- Biological function summary
In the human body creatinine plays an integral role in reflecting muscle metabolism rates and biochemical balance. Creatinine levels aid in assessing glomerular filtration rate (GFR) an important measure of kidney function. It is not part of a protein complex as it results from muscle activity. Its levels in blood and urine help calculate the protein to creatinine ratio providing insights into renal health. The constant exchange and excretion of creatinine make it a reliable biomarker in metabolism studies.
- Pathways
Muscle metabolism and kidney filtration both involve creatinine's function. Creatinine formation and elimination connect to pathways that maintain homeostasis and waste removal. Key proteins in these pathways include creatine kinase which catalyzes the conversion of creatine to creatine phosphate. The creatinine protein ratio becomes relevant in evaluating proteinuria a condition where excess protein is present in urine indicating possible kidney dysfunction.
- Associated diseases and disorders
Kidney-related conditions and muscle disorders often involve creatinine. Common health concerns like chronic kidney disease (CKD) associate with elevated creatinine levels indicating reduced kidney function. Additionally conditions such as muscular dystrophy can alter muscle creatinine levels. These diseases relate to proteins such as nephrin involved in the kidney filtration barrier and dystrophin essential for muscular integrity. For clinical assessments tools like the creatinine assay and understanding the protein creatinine ratio are key for early detection and management.
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10 product images
Functional Studies - Creatinine Assay Kit (ab65340)
Creatinine measured in mouse and human serum plotted against RFU. Samples were diluted 5-10 fold.
Gai, Zhibo et al., Scientific reports vol. 7,1 9815., Fig 2, doi:10.1038/s41598-017-10168-6Functional Studies - Creatinine Assay Kit (ab65340)
Mice were injected i.p. with 6-ECDCA before I/R injury. At 24 h after I/R, kidney, serum and urine samples were collected for measurements of creatinine in serum with ab65340
Functional Studies - Creatinine Assay Kit (ab65340)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Functional Studies - Creatinine Assay Kit (ab65340)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Functional Studies - Creatinine Assay Kit (ab65340)
Creatinine measured in mouse and human urine plotted against RFU. Samples were diluted 400-800 fold.
Immunodiffusion - Creatinine Assay Kit (ab65340)
Creatinine levels in filtered human urine was measured in the presence or absence of creatininase (background signal subtracted.
Biochemical assay - Creatinine Assay Kit (ab65340)
Creatinine Assay Kit ab65340 used with mouse plasma.
Sung et al used Creatinine Assay Kit ab65340 to measure differences in plasma creatinine levels between normoglycemia (NG) and diabetes mellitus (DM) PINK1 KO and normal mice.
Plasma samples were collected from each mouse at the time of sacrifice. Plasma creatinine levels were measured using a creatinine ELISA kit (Creatinine Assay kit, Abcam, ab65340, Cambridge, UK) following the manufacturer's instructions.
Biochemical assay - Creatinine Assay Kit (ab65340)
Mouse Albumin ELISA kit Mouse Albumin ELISA Kit ab108792 and Creatinine Assay Kit ab65340 used with mouse urine.
Szabo et al measured urine albumin and urine albumin creatinine ratio in wildtype, and Epcam and Tacstd2 null mice.
To collect urine, 2-3 week old mice were held over plastic sheet for up to 30 s with lightly stroking the belly to allow urination. Urine was then transferred into an Eppendorf tube, cleared by centrifugation, and stored at −80°C until analyzed. 1 µl of urine was used to measure albumin concentration by colorimetric ELISA assay (Mouse Albumin ELISA kit, Mouse Albumin ELISA Kit ab108792, Abcam) according to the manufacturer's protocol. To determine creatinine concentration, 2 µl of urine were used in a colorimetric assay (Creatinine Assay Kit, ab65340, Abcam). At the conclusion of the assay, absorbances were read on Synergy Neo microplate reader (Agilent Biotek, Santa Clara, CA, USA). All standards and samples were measured in duplicates and the concentrations were determined from the average of the two values.
Biochemical assay - Creatinine Assay Kit (ab65340)
Urea Assay Kit Urea Assay Kit ab83362 used to measure blood urea nitrogen with mouse serum, and Mouse Albumin ELISA kit Mouse Albumin ELISA Kit ab108792 and Creatinine Assay Kit ab65340 used to measure albumin-creatinine ratio with mouse urine.
Widjaja et al subjected IL11 null mice and wild type controls to acute kidney injury and ran a blood urea nitrogen assay (Urea Assay Kit Urea Assay Kit ab83362), and measured urine albumin-creatinine ratio using Mouse Albumin ELISA kit Mouse Albumin ELISA Kit ab108792 and Creatinine Assay Kit ab65340.
Red indicates acute kidney injury. AKI was induced by intraperitoneal (IP) injection of folic acid (FA, 200 mg/kg) in vehicle (0.3 M NaHCO3) to 10–12-week-old male mice; control mice were administered vehicle alone.
All ELISA and colorimetric assays were performed according to the manufacturer’s protocol.
Biochemical assay - Creatinine Assay Kit (ab65340)
Diagram showing the principles of the Creatinine assay method.
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