Creatinine Assay Kit ab65340 provides an accurate, convenient measure of creatinine concentration in biological fluids such as serum, urine or CSF.
Images
Publications
- Nature communications 15:3712024Deletion of Aurora kinase A prevents the development of polycystic kidney disease in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Shen Tham,Denny L Cottle,Allara K Zylberberg,Kieran M Short,Lynelle K Jones,Perkin Chan,Sarah E Conduit,Jennifer M Dyson,Christina A Mitchell,Ian M SmythPubMed 38191531
- Science advances 9:eadi47772023Adrenomedullin has a pivotal role in trophoblast differentiation: A promising nanotechnology-based therapeutic target for early-onset preeclampsia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingqing Zhang,Cheuk-Lun Lee,Tingyu Yang,Jianlin Li,Qunxiong Zeng,Xiaofeng Liu,Zhongzhen Liu,Degong Ruan,Zhuoxuan Li,Anita S Y Kan,Ka-Wang Cheung,Annisa S L Mak,Vivian W Y Ng,Huashan Zhao,Xiujun Fan,Yong-Gang Duan,Liuying Zhong,Min Chen,Meirong Du,Raymond H W Li,Pengtao Liu,Ernest H Y Ng,William S B Yeung,Ya Gao,Yuanqing Yao,Philip C N ChiuPubMed 37922358
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Cerebral Spinal Fluid, Other biological fluids, Serum, Urine
- Assay type
Quantitative
- Assay time
1h
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
What's included?
Recommended products
Creatinine Assay Kit ab65340 provides an accurate, convenient measure of creatinine concentration in biological fluids such as serum, urine or CSF.
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Cerebral Spinal Fluid, Other biological fluids, Serum, Urine
- Assay type
Quantitative
- Assay time
1h
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Creatinine Assay Kit ab65340 provides an accurate, convenient measure of creatinine concentration in biological fluids such as serum, urine or CSF.
In the creatinine assay protocol, creatinine is converted to creatine by creatininase, creatine is converted to sarcosine, which is specifically oxidized to produce a product which reacts with a probe to generate red color (λmax = 570 nm) and fluorescence (Ex/Em = 538/587 nm).
Unlike picric acid assays, this kit is suitable for serum creatinine determinations, as well as for urine and other biological samples.
Creatinine assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 60 min at 37°C
- analyze with microplate reader
For deproteinization of samples: Better results are typically seen with this assay when using a 10kda filter for sample deproteinization than when using the PCA method.
This product is manufactured by BioVision, an Abcam company and was previously called K625 Creatinine Colorimetric/Fluorometric Assay Kit. K625-100 is the same size as the 100 test size of ab65340.
Supplementary info
- Activity summary
Creatinine also known as 2-amino-1-methyl-5H-imidazol-4-one is a small molecule with a molecular mass of approximately 113.12 g/mol. Its mechanical functions stem from its role as a breakdown product of creatine phosphate primarily in muscle. Creatinine serves as a marker for kidney function due to its constant production and relatively stable concentration in the blood. It gets filtered out of the blood by the kidneys and is present in urine. Laboratories focus on measuring creatinine levels using tools like the creatinine assay kit and creatinine test kit to evaluate renal function.
- Biological function summary
In the human body creatinine plays an integral role in reflecting muscle metabolism rates and biochemical balance. Creatinine levels aid in assessing glomerular filtration rate (GFR) an important measure of kidney function. It is not part of a protein complex as it results from muscle activity. Its levels in blood and urine help calculate the protein to creatinine ratio providing insights into renal health. The constant exchange and excretion of creatinine make it a reliable biomarker in metabolism studies.
- Pathways
Muscle metabolism and kidney filtration both involve creatinine's function. Creatinine formation and elimination connect to pathways that maintain homeostasis and waste removal. Key proteins in these pathways include creatine kinase which catalyzes the conversion of creatine to creatine phosphate. The creatinine protein ratio becomes relevant in evaluating proteinuria a condition where excess protein is present in urine indicating possible kidney dysfunction.
- Associated diseases and disorders
Kidney-related conditions and muscle disorders often involve creatinine. Common health concerns like chronic kidney disease (CKD) associate with elevated creatinine levels indicating reduced kidney function. Additionally conditions such as muscular dystrophy can alter muscle creatinine levels. These diseases relate to proteins such as nephrin involved in the kidney filtration barrier and dystrophin essential for muscular integrity. For clinical assessments tools like the creatinine assay and understanding the protein creatinine ratio are key for early detection and management.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Gai, Zhibo et al., Scientific reports vol. 7,1 9815., Fig 2, doi:10.1038/s41598-017-10168-6Functional Studies - Creatinine Assay Kit (ab65340)
Mice were injected i.p. with 6-ECDCA before I/R injury. At 24 h after I/R, kidney, serum and urine samples were collected for measurements of creatinine in serum with ab65340
Functional Studies - Creatinine Assay Kit (ab65340)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Functional Studies - Creatinine Assay Kit (ab65340)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Functional Studies - Creatinine Assay Kit (ab65340)
Creatinine measured in mouse and human serum plotted against RFU. Samples were diluted 5-10 fold.
Functional Studies - Creatinine Assay Kit (ab65340)
Creatinine measured in mouse and human urine plotted against RFU. Samples were diluted 400-800 fold.
Immunodiffusion - Creatinine Assay Kit (ab65340)
Creatinine levels in filtered human urine was measured in the presence or absence of creatininase (background signal subtracted.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com