Creatinine Assay Kit

• Biochemical assay

Lab

Biochemical assay - Creatinine Assay Kit (AB65340)

Diagram showing the principles of the Creatinine assay method.

• Biochemical assay

PubMed

Biochemical assay - Creatinine Assay Kit (AB65340)

Creatinine Assay Kit ab65340 used with mouse plasma.

Sung et al used Creatinine Assay Kit ab65340 to measure differences in plasma creatinine levels between normoglycemia (NG) and diabetes mellitus (DM) PINK1 KO and normal mice.

Plasma samples were collected from each mouse at the time of sacrifice. Plasma creatinine levels were measured using a creatinine ELISA kit (Creatinine Assay kit, Abcam, ab65340, Cambridge, UK) following the manufacturer's instructions.

• FuncS

Lab

Functional Studies - Creatinine Assay Kit (AB65340)

Creatinine measured in mouse and human serum plotted against RFU. Samples were diluted 5-10 fold.

• FuncS

Lab

Functional Studies - Creatinine Assay Kit (AB65340)

Creatinine measured in mouse and human urine plotted against RFU. Samples were diluted 400-800 fold.

• FuncS

Unknown

Functional Studies - Creatinine Assay Kit (AB65340)

Creatinine levels in filtered human urine was measured in the presence or absence of creatininase (background signal subtracted).

• FuncS

Unknown

Functional Studies - Creatinine Assay Kit (AB65340)

Mice were injected i.p. with 6-ECDCA before I/R injury. At 24 h after I/R, kidney, serum and urine samples were collected for measurements of creatinine in serum with ab65340

Gai, Zhibo et al., Scientific reports vol. 7,1 9815., Fig 2, doi:10.1038/s41598-017-10168-6

• FuncS

Lab

Functional Studies - Creatinine Assay Kit (AB65340)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

• FuncS

Lab

Functional Studies - Creatinine Assay Kit (AB65340)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

• Biochemical assay

PubMed

Biochemical assay - Creatinine Assay Kit (AB65340)

Mouse Albumin ELISA kit ab108792 and Creatinine Assay Kit ab65340 used with mouse urine.

Szabo et al measured urine albumin and urine albumin creatinine ratio in wildtype, and Epcam and Tacstd2 null mice.

To collect urine, 2-3 week old mice were held over plastic sheet for up to 30 s with lightly stroking the belly to allow urination. Urine was then transferred into an Eppendorf tube, cleared by centrifugation, and stored at −80°C until analyzed. 1 µl of urine was used to measure albumin concentration by colorimetric ELISA assay (Mouse Albumin ELISA kit, ab108792, Abcam) according to the manufacturer's protocol. To determine creatinine concentration, 2 µl of urine were used in a colorimetric assay (Creatinine Assay Kit, ab65340, Abcam). At the conclusion of the assay, absorbances were read on Synergy Neo microplate reader (Agilent Biotek, Santa Clara, CA, USA). All standards and samples were measured in duplicates and the concentrations were determined from the average of the two values.

• Biochemical assay

PubMed

Biochemical assay - Creatinine Assay Kit (AB65340)

Urea Assay Kit ab83362 used to measure blood urea nitrogen with mouse serum, and Mouse Albumin ELISA kit ab108792 and Creatinine Assay Kit ab65340 used to measure albumin-creatinine ratio with mouse urine.

Widjaja et al subjected IL11 null mice and wild type controls to acute kidney injury and ran a blood urea nitrogen assay (Urea Assay Kit ab83362), and measured urine albumin-creatinine ratio using Mouse Albumin ELISA kit ab108792 and Creatinine Assay Kit ab65340.

Red indicates acute kidney injury. AKI was induced by intraperitoneal (IP) injection of folic acid (FA, 200 mg/kg) in vehicle (0.3 M NaHCO3) to 10–12-week-old male mice; control mice were administered vehicle alone.

All ELISA and colorimetric assays were performed according to the manufacturer’s protocol.

• Other

Supplier Data

Other - Creatinine Assay Kit (AB65340)

Representative image of Creatinine Assay Kit ab65340

Components shown from left to right :

- OxiRed Probe

- Creatinase

- Creatinine Standard

- Sarcosine Enzyme Mix

- Creatininase

- Assay Buffer 2

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.