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AB204537

Creatinine Assay Kit (Colorimetric)

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(18 Publications)

Creatinine Assay Kit (Colorimetric) (ab204537) is a complete kit for the quantitative determination of creatinine in urine.

Creatinine Assay Kit (Colorimetric) designed for success:
- Fast homogeneous mix-and-ready assay with no wash steps, results in just 30 minutes
- Calibrated against the NIST standard and offers reproducible results with less than 6% inter- and intra-assay variation.
- Highly sensitive, of which the detection limit can be as low as 0.042 mg/dL.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Functional Studies - Creatinine Assay Kit (Colorimetric) (AB204537)
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Supplier Data

Functional Studies - Creatinine Assay Kit (Colorimetric) (AB204537)

Typical standard curve obtained using Creatinine Assay Kit (Colorimetric) (ab204537) which is a complete kit for the quantitative determination of creatinine in urine, and is based upon the Jaffe reaction.

Key facts

Detection method

Colorimetric

Sample types

Urine

Assay type

Quantitative

Assay time

30m

Assay Platform

Microplate reader

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Product details

Creatinine Assay Kit (Colorimetric) (ab204537) is a complete kit for the quantitative determination of creatinine in urine.

Creatine assay method
Creatinine Assay Kit ab204537 is based upon the Jaffe reaction. Creatinine reacts with alkaline picrate to produce an orange color. The color intensity is measured before and after adding acid, and the difference is proportional to the creatinine concentration.

Creatinine Assay protocol summary:
- Add samples to wells
- Add reaction mix and incubate for 30 minutes at room temperature
- Measure optical density (OD490 nm)

Related and recommended products
See below other alternative Creatinine Assay Kits:
- Creatinine Assay Kit ab65340
- Albumin Creatinine Ratio Assay Kit ab241018
- Protein Creatinine Ratio Assay Kit ab272539

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Precision

[ { "reproducibilityType": "Inter", "sample": "1.932 mg/dL Spike", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.027" }, { "reproducibilityType": "Inter", "sample": "7.085 mg/dL Spike", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.433" }, { "reproducibilityType": "Inter", "sample": "7.232 mg/dL Spike", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.052" }, { "reproducibilityType": "Inter", "sample": "7.591 mg/dL Spike", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.249" }, { "reproducibilityType": "Intra", "sample": "2.007 mg/dL Spike", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.995" }, { "reproducibilityType": "Intra", "sample": "7.326 mg/dL Spike", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.729" }, { "reproducibilityType": "Intra", "sample": "7.452 mg/dL Spike", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.203" }, { "reproducibilityType": "Intra", "sample": "7.981 mg/dL Spike", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.159" } ]
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Recovery

[ { "sample": "Urine", "range": "92 - 99 %", "average": "= 95" } ]
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What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab204537-2x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Unit", "name":"Plate Sealer", "number":"AB204537-CMP04", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Clear Microtiter Plates", "number":"AB204537-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Creatinine Detection Reagent", "number":"AB204537-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Creatinine Standard", "number":"AB204537-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information creatinine
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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 16:544-556 PubMed38463588

2024

Dysregulation of kidney proteases in the pathogenesis of hypertension following unilateral nephrectomy in juvenile mice.

Applications

Unspecified application

Species

Unspecified reactive species

Rasha Aly,Yunus E Dogan,Niharika Bala,Carlos Lugo,Seena Darwish,Lawrence Shoemaker,Abdel A Alli

Communications biology 7:42 PubMed38182741

2024

Increased urinary creatinine during hibernation and day roosting in the Eastern bent-winged bat (Miniopterus fuliginosus) in Korea.

Applications

Unspecified application

Species

Unspecified reactive species

Heungjin Ryu,Kodzue Kinoshita,Sungbae Joo,Yu-Seong Choi,Sun-Sook Kim

Aging 15:10291-10306 PubMed37812195

2023

polysaccharide decreases podocyte injury in diabetic nephropathy by regulating IRS-1/AKT signal and promoting mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Huahua Li,Jin Zheng,Yacen Wu,Hong Zhou,Suli Zeng,Quanqing Li

Molecular medicine (Cambridge, Mass.) 29:92 PubMed37415117

2023

Nicotine exacerbates diabetic nephropathy through upregulation of Grem1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jianning Chen,Haiting Xiao,Rui Xue,Vinod Kumar,Rukhsana Aslam,Syed Faizan Mehdi,Huairong Luo,Ashwani Malhotra,Xiqian Lan,Pravin Singhal

Toxics 11: PubMed36977038

2023

Modulatory Role of Autophagy in Metformin Therapeutic Activity toward Doxorubicin-Induced Nephrotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Samar A Antar,Marwa Abd-Elsalam,Walied Abdo,Ahmed Abdeen,Mohamed Abdo,Liana Fericean,Nahed A Raslan,Samah F Ibrahim,Asmaa F Sharif,Amira Elalfy,Hend E Nasr,Ahmed B Zaid,Rania Atia,Ahmed M Atwa,Mohammed A Gebba,Amany A Alzokaky

Scientific reports 13:4111 PubMed36914734

2023

Renal toxicity and biokinetics models after repeated uranium instillation.

Applications

Unspecified application

Species

Unspecified reactive species

Laurie De Castro,Annabelle Manoury,Olivier Claude,Bastien Simoneau,Virginie Monceau,David Suhard,Christelle Elie,Victor Magneron,Laurence Roy,Céline Bouvier-Capely,Chrystelle Ibanez,Estelle Davesne,Yann Guéguen

Cell 185:3617-3636.e19 PubMed36070752

2022

Design, construction, and in vivo augmentation of a complex gut microbiome.

Applications

Unspecified application

Species

Unspecified reactive species

Alice G Cheng,Po-Yi Ho,Andrés Aranda-Díaz,Sunit Jain,Feiqiao B Yu,Xiandong Meng,Min Wang,Mikhail Iakiviak,Kazuki Nagashima,Aishan Zhao,Pallavi Murugkar,Advait Patil,Katayoon Atabakhsh,Allison Weakley,Jia Yan,Ariel R Brumbaugh,Steven Higginbottom,Alejandra Dimas,Anthony L Shiver,Adam Deutschbauer,Norma Neff,Justin L Sonnenburg,Kerwyn Casey Huang,Michael A Fischbach

Brazilian journal of biology = Revista brasleira de biologia 84:e258234 PubMed35830129

2022

Ameliorative potential role of Rosmarinus officinalis extract on toxicity induced by etoposide in male albino rats.

Applications

Unspecified application

Species

Unspecified reactive species

Reham M Morsi,Doaa S Mansour,Amr M Mousa

Frontiers in physiology 13:934597 PubMed35812319

2022

Combined Systemic Intake of K-ATP Opener (Nicorandil) and Mesenchymal Stem Cells Preconditioned With Nicorandil Alleviates Pancreatic Insufficiency in a Model of Bilateral Renal Ischemia/Reperfusion Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Asmaa Mohammed ShamsEldeen,Sarah A Abd El-Aal,Basma Emad Aboulhoda,Hend AbdAllah,Sara Mahmoud Gamal,Fatma E Hassan,Marwa Nagi Mehesen,Laila Ahmed Rashed,Abeer Mostafa,Nermeen Bakr Sadek

Kidney & blood pressure research 47:514-522 PubMed35717941

2022

Pterostilbene, a Resveratrol Derivative, Improves Ectopic Lipid Deposition in the Kidneys of Mice Induced by a High-Fat Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Gu,Linquan Yang,Xing Wang,Jianlin Geng,Xiaolong Li,Kunjie Zheng,Yunpeng Guan,Xiaoyu Hou,Chao Wang,Guangyao Song
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

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Alternative Product
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(2 reviews)

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