Crystal violet Assay Kit (Cell viability)

Product details

Crystal Violet Assay Kit ab232855 is used for cytoxicity and cell viability studies with adherent cell cultures.

Crystal violet assay principle

The Crystal Violet assay is based on staining cells that are attached to cell culture plates. It relies on the detachment of adherent cells from cell culture plates during cell death. During the assay, dead detached cells are washed away. The remaining attached live cells are stained with Crystal violet. After a wash step, the Crystal violet dye is solubilized and measured by absorbance at 570 nm.

The amount of Crystal Violet staining in the assay is directly proportional to the cell biomass that is attached to the plate. The cell biomass can be used to infer levels of cell viability / cytotoxicity. The Crystal Violet assay assumes that all cells that are attached to the plate are "alive" and that all cells that detach are "dead".

Crystal violet is a triarylmethane dye that binds to ribose type molecules such as DNA in nuclei.

Crystal Violet assay protocol summary:
- remove culture medium
- wash cells
- add Crystal Violet staining solution
- incubate for 20 mins
- wash cells and air dry plate
- add solubilization solution
- analyze using a microplate reader at 570 nm absorbance

REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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