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Crystal Violet Assay Kit ab232855 is used for cytoxicity and cell viability studies with adherent cell cultures.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

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Images

Key facts

Detection method

Colorimetric

Sample types

Adherent cells

Reactive species

Mammals

Assay time

1h

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Alternative names

What's included?

1000 Test
Components
10X Wash Buffer I
1 x 115 mL
CV Solubilization Solution
1 x 100 mL
CV Solution
1 x 40 mL
Doxorubicin
1 x 100 µL

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Crystal Violet Assay Kit ab232855 is used for cytoxicity and cell viability studies with adherent cell cultures.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Adherent cells

Reactive species

Mammals

Assay time

1h

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Crystal Violet Assay Kit ab232855 is used for cytoxicity and cell viability studies with adherent cell cultures.

The Crystal Violet assay is based on staining cells that are attached to cell culture plates. It relies on the detachment of adherent cells from cell culture plates during cell death. During the assay, dead detached cells are washed away. The remaining attached live cells are stained with Crystal violet. After a wash step, the Crystal violet dye is solubilized and measured by absorbance at 570 nm.

The amount of Crystal Violet staining in the assay is directly proportional to the cell biomass that is attached to the plate. The cell biomass can be used to infer levels of cell viability / cytotoxicity. The Crystal Violet assay assumes that all cells that are attached to the plate are "alive" and that all cells that detach are "dead".

Crystal violet is a triarylmethane dye that binds to ribose type molecules such as DNA in nuclei.

Crystal Violet assay protocol summary:
- remove culture medium
- wash cells
- add Crystal Violet staining solution
- incubate for 20 mins
- wash cells and air dry plate
- add solubilization solution
- analyze using a microplate reader at 570 nm absorbance

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2 product images

  • Microcytotoxicity testing - Crystal violet Assay Kit (Cell viability) (ab232855), expandable thumbnail

    Microcytotoxicity testing - Crystal violet Assay Kit (Cell viability) (ab232855)

    Dose-response curve of MCF7 (Human breast adenocarcinoma cell line) cells to Doxorubicin for 72 hours determined by the Crystal violet staining assay. Assays were performed according to the kit protocol in triplicate.

  • Microcytotoxicity testing - Crystal violet Assay Kit (Cell viability) (ab232855), expandable thumbnail

    Microcytotoxicity testing - Crystal violet Assay Kit (Cell viability) (ab232855)

    Dose-response curve of HepG2 (Human liver hepatocellular carcinoma cell line) cells to Doxorubicin for 72 hours determined by the Crystal violet staining assay. Assays were performed according to the kit protocol in triplicate.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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