Cyclooxygenase COX Activity Assay Kit (Fluorometric) (ab204699) provides a simple, sensitive, and high-throughput adaptable method to detect the peroxidase activity of COX in biological samples or purified/crude enzyme preparations.
- Fluorometric readout (535/587 nm) - works on microplate-reader
- includes COX-1 and COX-2 specific inhibitors to differentiate the activity of COX-1 and COX-2
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Scientific reports 13:194712023Orai1α and Orai1β support calcium entry and mammosphere formation in breast cancer stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsaac Jardin,Sandra Alvarado,Vanesa Jimenez-Velarde,Joel Nieto-Felipe,Jose J Lopez,Gines M Salido,Tarik Smani,Juan A RosadoPubMed 37945647
- Function (Oxford, England) 4:zqad0532023Inactivation of TRPM7 Kinase Targets AKT Signaling and Cyclooxygenase-2 Expression in Human CML Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBirgit Hoeger,Wiebke Nadolni,Sarah Hampe,Kilian Hoelting,Marco Fraticelli,Nadja Zaborsky,Anna Madlmayr,Viktoria Sperrer,Laura Fraticelli,Lynda Addington,Dirk Steinritz,Vladimir Chubanov,Roland Geisberger,Richard Greil,Andreas Breit,Ingrid Boekhoff,Thomas Gudermann,Susanna ZierlerPubMed 37786778
- Pharmaceutics 14:2022Enhanced Antioxidant Effects of the Anti-Inflammatory Compound Probucol When Released from Mesoporous Silica Particles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael Lau,Benjamin Sealy,Valery Combes,Marco Morsch,Alfonso E Garcia-BennettPubMed 35335878
- Avicenna journal of phytomedicine 12:89-1002022attenuates neuroinflammation in an animal model of Alzheimer's disease through modulation of nuclear factor κB pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSoomaayeh Heysieattalab,Leila SadeghiPubMed 35145898
- Cells 11:2022Effect of Withanolide A on 7-Ketocholesterol Induced Cytotoxicity in hCMEC/D3 Brain Endothelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandra Soh,Wei-Yi OngPubMed 35159267
- Frontiers in pharmacology 12:7150762021Structural Modifications Yield Novel Insights Into the Intriguing Pharmacodynamic Potential of Anti-inflammatory Nitro-Fatty Acids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNadine Hellmuth,Camilla Brat,Omar Awad,Sven George,Astrid Kahnt,Tom Bauer,Hai Phong Huynh Phuoc,Dieter Steinhilber,Carlo Angioni,Mohamed Hassan,Katharina J Hock,Georg Manolikakes,Kai Zacharowski,Jessica Roos,Thorsten J MaierPubMed 34867322
- International journal of immunopathology and pharmacology 35:205873842110346862021Anti-inflammatory properties of a proprietary bromelain extract (Bromeyal™) after simulated gastrointestinal digestion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoberta Bottega,Ilaria Persico,Francesco De Seta,Federico Romano,Giovanni Di LorenzoPubMed 34387509
- Frontiers in bioengineering and biotechnology 9:6976712021Indomethacin Treatment Post-irradiation Improves Mouse Parotid Salivary Gland Function via Modulation of Prostaglandin E Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKristy E Gilman,Jean M Camden,Lucas T Woods,Gary A Weisman,Kirsten H LimesandPubMed 34381764
- Molecules (Basel, Switzerland) 26:2021Toward Multitasking Pharmacological COX-Targeting Agents: Non-Steroidal Anti-Inflammatory Prodrugs with Antiproliferative Effects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFedora Grande,Francesca Giordano,Maria Antonietta Occhiuzzi,Carmine Rocca,Giuseppina Ioele,Michele De Luca,Gaetano Ragno,Maria Luisa Panno,Bruno Rizzuti,Antonio GarofaloPubMed 34203324
- Journal of tissue engineering and regenerative medicine 14:1929-19382020COX-2 inhibition does not alter wound healing outcomes of a volumetric muscle loss injury treated with a biologic scaffold.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephen M Goldman,Michael S Valerio,Naveena B Janakiram,Christopher L DearthPubMed 33049117
Key facts
- Detection method
- Fluorescent
- Sample types
- Tissue, Suspension cells, Adherent cells
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Assay time
- 45m
Target data
Function
Dual cyclooxygenase and peroxidase that plays an important role in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate, AA, C20:4(n-6)), with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates AA to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide prostaglandin H2 (PGH2), the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons (PubMed:7947975). Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells (Probable). Can also use linoleate (LA, (9Z,12Z)-octadecadienoate, C18:2(n-6)) as substrate and produce hydroxyoctadecadienoates (HODEs) in a regio- and stereospecific manner, being (9R)-HODE ((9R)-hydroxy-(10E,12Z)-octadecadienoate) and (13S)-HODE ((13S)-hydroxy-(9Z,11E)-octadecadienoate) its major products (By similarity).
Additional Targets
PTGS2
Alternative names
COX1, PTGS1, Prostaglandin G/H synthase 1, Cyclooxygenase-1, Prostaglandin H2 synthase 1, Prostaglandin-endoperoxide synthase 1, COX-1, PGH synthase 1, PGHS-1, PHS 1
What's included?
Recommended products
Cyclooxygenase COX Activity Assay Kit (Fluorometric) (ab204699) provides a simple, sensitive, and high-throughput adaptable method to detect the peroxidase activity of COX in biological samples or purified/crude enzyme preparations.
- Fluorometric readout (535/587 nm) - works on microplate-reader
- includes COX-1 and COX-2 specific inhibitors to differentiate the activity of COX-1 and COX-2
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Fluorescent
- Sample types
- Tissue, Suspension cells, Adherent cells
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Assay time
- 45m
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Cyclooxygenase COX Activity Assay Kit (Fluorometric) (ab204699) provides a simple, sensitive, and high-throughput adaptable method to detect the peroxidase activity of COX in biological samples or purified/crude enzyme preparations. The kit includes COX-1 and COX-2 specific inhibitors to differentiate the activity of COX-1 and COX-2 as well as other peroxidases, which may be present in the sample. Detection limit: 6 μU/mg.
How the Cox activity assay works
Cyclooxygenase (COX) Activity Assay Kit ab204699 measures COX peroxidase activity by detecting the conversion of arachidonic acid to prostaglandin G2 (PGG2), which reacts with a COX probe to produce a fluorescent signal proportional to enzyme activity.
Cyclooxygenase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- add arachidonic acid/NaOH solution
- analyze with microplate reader every 15 s for 30 min
This product is manufactured by BioVision, an Abcam company and was previously called K549 Cyclooxygenase (COX) Activity Assay Kit (Fluorometric). K549-100 is the same size as the 100 test size of ab204699.
Cyclooxygenase (COX), also known as prostaglandin-endoperoxide synthase (PTGS, EC 1.14.99.1), is an enzyme that is responsible for the formation of important biological mediators called prostanoids, including prostaglandins, prostacyclin and thromboxane. COX is the central enzyme in the biosynthetic pathway to prostanoids from arachidonic acid. There are two known isoenzymes: COX-1 and COX-2. COX-1 is constitutively expressed in many tissues and is the predominant form in gastric mucosa and in kidney. COX-2 is not expressed under normal conditions in most cells, but elevated levels are observed during inflammation. Pharmacological inhibition of COX by nonsteroidal anti-inflammatory drugs (NSAID) can provide relief from the symptoms of inflammation and pain.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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4 product images
Functional Studies - Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (ab204699)
Measurement of COX-1 activity in absence (blue line) and presence of COX-1 inhibitor (SC560, red line) using the COS-1 positive control (PC) provided in the kit.
Functional Studies - Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (ab204699)
Typical Resorfurin Standard calibration curve.
Functional Studies - Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (ab204699)
Detection of endogenous COX-1 activity in J774 cell lysate (6 μg) and rat liver homogenate (210 μg).
Functional Studies - Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (ab204699)
Detection of endogenous COX-2 activity in J774 cell lysate (7 μg) stimulated with or without 100 ng/ml LPS and 100 ng/ml murine IFN-gamma.
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- Visit the general protocols
- Visit troubleshooting
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