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AB204699

Cyclooxygenase (COX) Activity Assay Kit (Fluorometric)

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(24 Publications)

Cyclooxygenase COX Activity Assay Kit (Fluorometric) (ab204699) provides a simple, sensitive, and high-throughput adaptable method to detect the peroxidase activity of COX in biological samples or purified/crude enzyme preparations.

- Fluorometric readout (535/587 nm) - works on microplate-reader
- includes COX-1 and COX-2 specific inhibitors to differentiate the activity of COX-1 and COX-2
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

COX1, PTGS1, Prostaglandin G/H synthase 1, Cyclooxygenase-1, Prostaglandin H2 synthase 1, Prostaglandin-endoperoxide synthase 1, COX-1, PGH synthase 1, PGHS-1, PHS 1, COX2, PTGS2, Prostaglandin G/H synthase 2, Cyclooxygenase-2, PHS II, Prostaglandin H2 synthase 2, Prostaglandin-endoperoxide synthase 2, COX-2, PGH synthase 2, PGHS-2

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Functional Studies - Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (AB204699)
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Supplier Data

Functional Studies - Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (AB204699)

Measurement of COX-1 activity in absence (blue line) and presence of COX-1 inhibitor (SC560, red line) using the COS-1 positive control (PC) provided in the kit.

Functional Studies - Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (AB204699)
  • FuncS

Supplier Data

Functional Studies - Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (AB204699)

Detection of endogenous COX-1 activity in J774 cell lysate (6 μg) and rat liver homogenate (210 μg).

Functional Studies - Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (AB204699)
  • FuncS

Supplier Data

Functional Studies - Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (AB204699)

Detection of endogenous COX-2 activity in J774 cell lysate (7 μg) stimulated with or without 100 ng/ml LPS and 100 ng/ml murine IFN-gamma.

Functional Studies - Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (AB204699)
  • FuncS

Supplier Data

Functional Studies - Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (AB204699)

Typical Resorfurin Standard calibration curve.

Key facts

Detection method

Fluorescent

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Enzyme activity

Assay time

45m

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Cyclooxygenase COX Activity Assay Kit (Fluorometric) (ab204699) provides a simple, sensitive, and high-throughput adaptable method to detect the peroxidase activity of COX in biological samples or purified/crude enzyme preparations. The kit includes COX-1 and COX-2 specific inhibitors to differentiate the activity of COX-1 and COX-2 as well as other peroxidases, which may be present in the sample. Detection limit: 6 μU/mg.

How the Cox activity assay works

Cyclooxygenase (COX) Activity Assay Kit ab204699 measures COX peroxidase activity by detecting the conversion of arachidonic acid to prostaglandin G2 (PGG2), which reacts with a COX probe to produce a fluorescent signal proportional to enzyme activity.

Cyclooxygenase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- add arachidonic acid/NaOH solution
- analyze with microplate reader every 15 s for 30 min

This product is manufactured by BioVision, an Abcam company and was previously called K549 Cyclooxygenase (COX) Activity Assay Kit (Fluorometric). K549-100 is the same size as the 100 test size of ab204699.

Cyclooxygenase (COX), also known as prostaglandin-endoperoxide synthase (PTGS, EC 1.14.99.1), is an enzyme that is responsible for the formation of important biological mediators called prostanoids, including prostaglandins, prostacyclin and thromboxane. COX is the central enzyme in the biosynthetic pathway to prostanoids from arachidonic acid. There are two known isoenzymes: COX-1 and COX-2. COX-1 is constitutively expressed in many tissues and is the predominant form in gastric mucosa and in kidney. COX-2 is not expressed under normal conditions in most cells, but elevated levels are observed during inflammation. Pharmacological inhibition of COX by nonsteroidal anti-inflammatory drugs (NSAID) can provide relief from the symptoms of inflammation and pain.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab204699-100Test", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"SC560 (COX-1 Inhibitor)", "number":"AB204699-CMP09", "productcode":"" }, { "size":"1 x 50 µL", "name":"Resorufin Standard", "number":"AB204699-CMP08", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB204699-CMP05", "productcode":"AB309437" }, { "size":"1 x 500 µL", "name":"NaOH Solution", "number":"AB204699-CMP07", "productcode":"" }, { "size":"1 x 100 µL", "name":"Celecoxib (COX-2 Inhibitor)", "number":"AB204699-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"COX-1 Positive Control", "number":"AB204699-CMP04", "productcode":"" }, { "size":"1 x 20 µL", "name":"COX Cofactor", "number":"AB204699-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"COX Assay Buffer", "number":"AB204699-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Arachidonic Acid", "number":"AB204699-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Dual cyclooxygenase and peroxidase that plays an important role in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate, AA, C20 : 4(n-6)), with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates AA to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide prostaglandin H2 (PGH2), the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons (PubMed : 7947975). Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells (Probable). Can also use linoleate (LA, (9Z,12Z)-octadecadienoate, C18 : 2(n-6)) as substrate and produce hydroxyoctadecadienoates (HODEs) in a regio- and stereospecific manner, being (9R)-HODE ((9R)-hydroxy-(10E,12Z)-octadecadienoate) and (13S)-HODE ((13S)-hydroxy-(9Z,11E)-octadecadienoate) its major products (By similarity).
See full target information PTGS1

Additional targets

PTGS2
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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Inorganic chemistry 63:19769-19782 PubMed39389034

2024

Gold(I) Complexes Based on Nonsteroidal Anti-Inflammatory Derivatives as Multi-Target Drugs against Colon Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Javier Saez,Javier Quero,María Jesús Rodriguez-Yoldi,M Concepción Gimeno,Elena Cerrada

Journal of cachexia, sarcopenia and muscle 15:562-574 PubMed38302863

2024

R-ketorolac ameliorates cancer-associated cachexia and prolongs survival of tumour-bearing mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sophia E Chrysostomou,Sandra Eder,Isabella Pototschnig,Anna-Lena Mayer,Martina Derler,Marion Mussbacher,Silvia Schauer,Dongxu Zhang,Dongmei Yan,Gennie Liu,Gerald Hoefler,Thomas Weichhart,Paul W Vesely,Lingbing Zhang,Martina Schweiger

Antioxidants (Basel, Switzerland) 13: PubMed38247536

2024

Effects of Particulate Matter Inhalation during Exercise on Oxidative Stress and Mitochondrial Function in Mouse Skeletal Muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Jinhan Park,Junho Jang,Byunghun So,Kanggyu Lee,Dongjin Yeom,Ziyi Zhang,Woo Shik Shin,Chounghun Kang

mBio 15:e0306523 PubMed38190129

2024

Suppression of progesterone by influenza A virus mediates adverse maternal and fetal outcomes in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick S Creisher,Maclaine A Parish,Jun Lei,Jin Liu,Jamie L Perry,Ariana D Campbell,Morgan L Sherer,Irina Burd,Sabra L Klein

Scientific reports 13:19471 PubMed37945647

2023

Orai1α and Orai1β support calcium entry and mammosphere formation in breast cancer stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Isaac Jardin,Sandra Alvarado,Vanesa Jimenez-Velarde,Joel Nieto-Felipe,Jose J Lopez,Gines M Salido,Tarik Smani,Juan A Rosado

Function (Oxford, England) 4:zqad053 PubMed37786778

2023

Inactivation of TRPM7 Kinase Targets AKT Signaling and Cyclooxygenase-2 Expression in Human CML Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Birgit Hoeger,Wiebke Nadolni,Sarah Hampe,Kilian Hoelting,Marco Fraticelli,Nadja Zaborsky,Anna Madlmayr,Viktoria Sperrer,Laura Fraticelli,Lynda Addington,Dirk Steinritz,Vladimir Chubanov,Roland Geisberger,Richard Greil,Andreas Breit,Ingrid Boekhoff,Thomas Gudermann,Susanna Zierler

Pharmaceutics 14: PubMed35335878

2022

Enhanced Antioxidant Effects of the Anti-Inflammatory Compound Probucol When Released from Mesoporous Silica Particles.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Lau,Benjamin Sealy,Valery Combes,Marco Morsch,Alfonso E Garcia-Bennett

Avicenna journal of phytomedicine 12:89-100 PubMed35145898

2022

attenuates neuroinflammation in an animal model of Alzheimer's disease through modulation of nuclear factor κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Soomaayeh Heysieattalab,Leila Sadeghi

Cells 11: PubMed35159267

2022

Effect of Withanolide A on 7-Ketocholesterol Induced Cytotoxicity in hCMEC/D3 Brain Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Soh,Wei-Yi Ong

Frontiers in pharmacology 12:715076 PubMed34867322

2021

Structural Modifications Yield Novel Insights Into the Intriguing Pharmacodynamic Potential of Anti-inflammatory Nitro-Fatty Acids.

Applications

Unspecified application

Species

Unspecified reactive species

Nadine Hellmuth,Camilla Brat,Omar Awad,Sven George,Astrid Kahnt,Tom Bauer,Hai Phong Huynh Phuoc,Dieter Steinhilber,Carlo Angioni,Mohamed Hassan,Katharina J Hock,Georg Manolikakes,Kai Zacharowski,Jessica Roos,Thorsten J Maier
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