Cystathionine beta Synthase Assay Kit (ab241043) uses cysteine and homocysteine as substrates to produce H2S.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Publications
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- The Journal of nutrition 151:1407-14152021Letrozole-Induced Polycystic Ovary Syndrome Attenuates Cystathionine-β Synthase mRNA and Protein Abundance in the Ovaries of Female Sprague Dawley Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmanda E Bries,Joseph L Webb,Brooke Vogel,Claudia Carrillo,Aileen F Keating,Samantha K Pritchard,Gina Roslan,Joshua W Miller,Kevin L SchalinskePubMed 33758914
Key facts
- Detection method
Fluorescent
- Sample types
Tissue, Suspension cells, Cell culture supernatant, Adherent cells
Target data
Function
Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine (PubMed:23981774, PubMed:20506325, PubMed:23974653). Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons (By similarity).
Alternative names
Cystathionine beta-synthase, Beta-thionase, Serine sulfhydrase, CBS
What's included?
Recommended products
Cystathionine beta Synthase Assay Kit (ab241043) uses cysteine and homocysteine as substrates to produce H2S.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Cystathionine beta-synthase, Beta-thionase, Serine sulfhydrase, CBS
Key facts
- Detection method
Fluorescent
- Sample types
Tissue, Suspension cells, Cell culture supernatant, Adherent cells
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Cystathionine beta Synthase Assay Kit (ab241043) utilizes cysteine and homocysteine as substrates to produce H2S. Hydrogen sulfide reacts with the azido-functional group of the fluorescent probe yielding a fluorescent amino group (Ex/Em = 368/460 nm). The assay is highly sensitive, has a simple easy-to-follow protocol, and can detect as low as 1.45 mU of CβS activity.
This product is manufactured by BioVision, an Abcam company and was previously called K998 Cystathionine ß Synthase Activity Assay Kit (Fluorometric). K998-100 is the same size as the 100 test size of ab241043.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cystathionine beta-synthase (CBS) is an enzyme that catalyzes the conversion of homocysteine and serine into cystathionine. It is sometimes referred to as CBS monelyne or cystathionine synthase. CBS is a heme-containing protein with a molecular mass of approximately 63 kDa. It is expressed widely in tissues including the liver brain and kidney. The activity of CBS is regulated by various factors including the availability of cofactors like pyridoxal 5'-phosphate and adenosine triphosphate (ATP).
- Biological function summary
The enzyme CBS plays a role in the transsulfuration pathway where it helps in the metabolism of homocysteine. CBS is a part of a larger complex in some tissues where it interacts with other enzymes involved in sulfur amino acid metabolism. This function is important in maintaining cellular sulfur amino acid balance and protecting cells from oxidative stress caused by elevated levels of homocysteine.
- Pathways
CBS functions in the transsulfuration pathway which connects the methionine cycle and the synthesis of glutathione. This pathway is essential for detoxification and antioxidant protection. CBS interrelates with other proteins like cystathionine gamma-lyase (CGL) within this pathway. The interplay between CBS and CGL ensures the conversion of homocysteine to cysteine which is a precursor for the synthesis of glutathione.
- Associated diseases and disorders
CBS deficiency is linked to homocystinuria a disorder characterized by high levels of homocysteine in the blood and urine. Symptoms include cardiovascular problems and developmental delays. Furthermore alterations in CBS activity relate to cardiovascular diseases due to hyperhomocysteinemia. Within these conditions CBS interacts with other proteins involved in homocysteine metabolism such as methionine synthase which also contributes to the complexity of these disorders.
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3 product images
Functional Studies - Cystathionine beta Synthase Assay Kit (ab241043)
CβS Standard Curve.
CβS Standard Curve.
Functional Studies - Cystathionine beta Synthase Assay Kit (ab241043)
CβS activity in Liver Lysate.
CβS activity in Liver Lysate (20 μg) & Positive Control (1.5 μg).
Functional Studies - Cystathionine beta Synthase Assay Kit (ab241043)
CβS specific activity.
CβS specific activity in HepG2 cell lysate and Liver lysate. Assays were performed following the kit protocol.
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