AB110417

Cytochrome c Apoptosis ICC Antibody Kit

Name: Cytochrome c Apoptosis ICC Antibody Kit (ab110417) | Abcam
Brand: Abcam Limited
SKU: AB110417
Price: 535 USD
Availability: InStock

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(11 Publications)

Apoptotic cells which have released mitochondrial cytochrome c into the cytosol can be differentiated from non apoptotic cells which still retain cytochrome c in their mitochondria by fluorescence microscopy.

View Alternative Names

CYC, CYCS, Cytochrome c

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Immunocytochemistry/ Immunofluorescence - Cytochrome c Apoptosis ICC Antibody Kit (AB110417)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Cytochrome c Apoptosis ICC Antibody Kit (AB110417)

Immunocytochemistry/ Immunofluorescence - ApoTrack™ Cytochrome c Apoptosis ICC Antibody Kit (ab110417)

Key facts

Detection method

Fluorescent

Sample types

Adherent cells

Reacts with

Mouse, Rat, Human

Assay type

Direct

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

ab110417 (MSA07) contains a cytochrome c monoclonal antibody (50 μg) and an isotype-specific secondary antibody (50 μg) which has a fluorescein fluorophore conjugated to it. The kit also contains an ATP synthase V subunit alpha monoclonal antibody (50 μg) and the appropriate isotype-specific secondary antibody (50 μg) which has a Texas Red fluorophore conjugated to it.

ATP synthase is an inner mitochondrial membrane protein and serves as a mitochondrial marker since it is not released into the cytoplasm during apoptosis, unlike cytochrome c. Thus one is able to observe cytochrome c and a mitochondrial marker (ATP synthase subunit alpha) simultaneously by fluorescence microscopy using ab110417. Buffers and blocking solution for immunocytochemical analysis are also included in the kit.

This product was previously called ApoTrack™ Cytochrome c Apoptosis ICC Antibody Kit.**Other apoptosis assays**For more apoptosis assays, review the .

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome c commonly referred to as 'cyt c' or 'apotrack' is a small heme protein with a molecular mass of around 12 kDa. It gets expressed in the mitochondria of eukaryotic cells. During the process of apoptosis cytochrome c translocates from the mitochondria to the cytoplasm. This movement acts as an important step in the initiation of apoptosis. It's possible to identify this protein through western blot techniques often indicated by 'cyt c WB' or 'cytochrome c western blot'.

Biological function summary

Cytochrome c plays an integral role in both the respiratory chain and the intrinsic pathway of apoptosis. It functions as an electron carrier in the mitochondrial electron transport chain facilitating ATP production. In apoptosis cytochrome c partners with Apaf-1 to form the apoptosome complex. This complex is essential for activating caspase-9 a critical executer of the cell death process. Understanding its presence in these complexes is fundamental for studying programmed cell death mechanisms.

Pathways

Cytochrome c has significant roles within the mitochondrial apoptosis pathway. This process involves a series of molecular events leading to programmed cell death. Cytochrome c initiates the activation of the caspase cascade particularly influencing the work of proteins like caspase-3. It also links to the oxidative phosphorylation pathway where cytochrome c facilitates electron transfer between complex III and complex IV critical for ATP synthesis. These interactions highlight cytochrome c's multifaceted function in cellular metabolism and apoptosis.

Cytochrome c's displacement from the mitochondria connects to conditions like cancer and neurodegenerative diseases. Aberrant regulation of apoptosis can lead to uncontrolled cell growth in cancers with cytochrome c's release being a pivotal factor. In neurodegenerative diseases such as Parkinson's disease mitochondrial dysfunctions and altered apoptosis involving cytochrome c are observed. Additionally cytochrome c's relationship with Bcl-2 family proteins illustrates how it's embedded in the balance of pro-apoptotic and anti-apoptotic signals influencing disease progression.

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Product protocols

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Target data

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

See full target information CYCS

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed37408275

2023

Effect of Photobiomodulation on Protein Kinase Cδ, Cytochrome C, and Mitochondria in U87 MG Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Viktória Pevná,Georges Wagnières,Daniel Jancura,Veronika Huntošová

Oncology reports 48: PubMed35856441

2022

Administration of 4‑hexylresorcinol increases p53‑mediated transcriptional activity in oral cancer cells with the p53 mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Yei-Jin Kang,Won-Geun Yang,Weon-Sik Chae,Dae-Won Kim,Seong-Gon Kim,Horatiu Rotaru

Small (Weinheim an der Bergstrasse, Germany) 18:e2200710 PubMed35304816

2022

7-Dehydrocholesterol Encapsulated Polymeric Nanoparticles As a Radiation-Responsive Sensitizer for Enhancing Radiation Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ian Delahunty,Jianwen Li,Wen Jiang,Chaebin Lee,Xueyuan Yang,Anil Kumar,Zhi Liu,Weizhong Zhang,Jin Xie

Nanoscale 13:9252-9263 PubMed33982686

2021

Ultrasmall Gd@Cdots as a radiosensitizing agent for non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chaebin Lee,Xiangji Liu,Weizhong Zhang,Michael A Duncan,Fangchao Jiang,Christine Kim,Xuefeng Yan,Yong Teng,Hui Wang,Wen Jiang,Zibo Li,Jin Xie

Cancer letters 431:31-42 PubMed29807112

2018

Pinocembrin induces ER stress mediated apoptosis and suppresses autophagy in melanoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yufei Zheng,Kai Wang,Yuqi Wu,Yifan Chen,Xi Chen,Chenyue W Hu,Fuliang Hu

Neuroscience letters 561:134-9 PubMed24287378

2013

17β-estradiol protects cerebellar granule cells against β-amyloid-induced toxicity via the apoptotic mitochondrial pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Maddalena Napolitano,Loredana Costa,Roberto Piacentini,Claudio Grassi,Antonio Lanzone,Alberto Gulino

Food and chemical toxicology : an international jo 62:817-24 PubMed24161488

2013

Extra virgin olive oil potentiates the effects of aromatase inhibitors via glutathione depletion in estrogen receptor-positive human breast cancer (MCF-7) cells.

Applications

Unspecified application

Species

Human

Amar Mohamed Ismail,Lionel L A In,Mohammad Tasyriq,Devi Rosmy Syamsir,Khalijah Awang,Ayda Hussein Omer Mustafa,Omer Fadul Idris,Imad Fadl-Elmula,Noor Hasima

The Journal of biological chemistry 285:4025-4037 PubMed20007710

2009

The mitochondrial pathway of anesthetic isoflurane-induced apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yiying Zhang,Yuanlin Dong,Xu Wu,Yan Lu,Zhipeng Xu,Andrew Knapp,Yun Yue,Tiejun Xu,Zhongcong Xie

PloS one 3:e2455 PubMed18560593

2008

PINK1 is necessary for long term survival and mitochondrial function in human dopaminergic neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Alison Wood-Kaczmar,Sonia Gandhi,Zhi Yao,Andrey Y Abramov,Andrey S Y Abramov,Erik A Miljan,Gregory Keen,Lee Stanyer,Iain Hargreaves,Kristina Klupsch,Emma Deas,Julian Downward,Louise Mansfield,Parmjit Jat,Joanne Taylor,Simon Heales,Michael R Duchen,David Latchman,Sarah J Tabrizi,Nicholas W Wood

Molecular pharmacology 71:695-703 PubMed17158200

2006

The nucleotide analog cidofovir suppresses basic fibroblast growth factor (FGF2) expression and signaling and induces apoptosis in FGF2-overexpressing endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Liekens,Sofie Gijsbers,Els Vanstreels,Dirk Daelemans,Erik De Clercq,Sigrid Hatse

View all publications

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Product promise

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Cytochrome c Apoptosis ICC Antibody Kit

Immunocytochemistry/ Immunofluorescence - Cytochrome c Apoptosis ICC Antibody Kit (AB110417)

Key facts

Detection method

Sample types

Reacts with

Assay type

Complementary Products

Reagents, Controls & Accessories

Assay Kits

Assay Kits

Assay Kits

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise