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AB239711

Cytochrome C Oxidase Assay Kit

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(10 Publications)

Cytochrome C Oxidase Activity Assay Kit (ab239711) is simple, fast and high-throughput adaptable.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

COX4, COX4I1, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1

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Functional Studies - Cytochrome C Oxidase Assay Kit (AB239711)
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Functional Studies - Cytochrome C Oxidase Assay Kit (AB239711)

Cytochrome Oxidase Activity.

Purified mitochondria were used to calculate a decrease in OD at 550 nm (0.625-5 μg/reaction). In Blank, no change in the OD was observed. Rate is calculated by subtracting the initial OD reading from the final OD, t1 and t2 represents linear rate of reaction.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Tissue, Cell culture supernatant, Cell Lysate

Assay Platform

Microplate reader

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Secondary Antibodies

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Reactivity data

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Product details

Cytochrome C Oxidase Activity Assay Kit (ab239711) is simple, fast and high-throughput adaptable. This assay kit can be used for purified mitochondria or tissue extracts containing mitochondria. The kit provides for the fast and simple measurement of Cytochrome c oxidase activity on a 96-well plate format. The activity of the enzyme is determined colorimetrically by following the oxidation of reduced Cytochrome c as an absorbance decrease at 550 nm.

Other Notes
This product was previously called K287 Biovision Cytochrome Oxidase Activity Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complex IV also known as cytochrome c oxidase is an important component of the electron transport chain in mitochondria. This enzyme complex has significant mass approximately 204 kDa and operates primarily in the inner mitochondrial membrane. It catalyzes the transfer of electrons from cytochrome c to oxygen facilitating ATP generation. Complex IV is ubiquitously expressed in all tissues with higher expression in organs with high energy demands like heart and skeletal muscle. This enzyme is composed of multiple subunits encoded by both mitochondrial and nuclear DNA.
Biological function summary

Cytochrome c oxidase plays a critical role in cellular respiration. It forms part of the larger enzyme complex which also includes Complex I II and III. These complexes work together to create a proton gradient across the inner mitochondrial membrane essential for ATP synthesis via oxidative phosphorylation. This enzyme's activity is measured through assays like the cytochrome c oxidase assay which evaluates its function in various tissues. These assays help researchers understand how effectively electrons are being transferred and protons are driven across the membrane.

Pathways

Complex IV is integral to the oxidative phosphorylation pathway and the broader mitochondrial respiratory chain. It interacts closely with cytochrome c a small heme protein that shuttles electrons between Complex III (cytochrome c reductase) and Complex IV. Through these interactions the proton gradient is established enabling ATP synthase to convert chemical energy into usable cell energy. This process significantly impacts cellular metabolism and energy production influencing how efficiently cells function.

Complex IV dysfunction is associated with mitochondrial diseases and certain neurodegenerative disorders. For instance defects in cytochrome c oxidase lead to conditions like Leigh syndrome a severe neurological disorder. Also studies show that disruptions in the electron transport chain involving Complex IV relate closely to Alzheimer's disease. Alterations in proteins like cytochrome c which work in tandem with Complex IV can exacerbate these conditions highlighting the importance of this target in understanding and potentially treating these diseases.
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Product protocols

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Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
See full target information COX4I1
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40076489

2025

Integrin-Linked Kinase (ILK) Promotes Mitochondrial Dysfunction by Decreasing CPT1A Expression in a Folic Acid-Based Model of Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Mariano de la Serna-Soto,Laura Calleros,María Martos-Elvira,Ariadna Moreno-Piedra,Sergio García-Villoria,Mercedes Griera,Elena Alcalde-Estévez,Ana Asenjo-Bueno,Diego Rodríguez-Puyol,Sergio de Frutos,María Piedad Ruiz-Torres

Biology of sex differences 15:95 PubMed39587693

2024

Age- and sex-associated alterations in hypothalamic mitochondrial bioenergetics and inflammatory-associated signaling in the 3xTg mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Aida Adlimoghaddam,Kyle M Fontaine,Benedict C Albensi

Science advances 10:eadl1584 PubMed39110797

2024

KAT2A and KAT2B prevent double-stranded RNA accumulation and interferon signaling to maintain intestinal stem cell renewal.

Applications

Unspecified application

Species

Unspecified reactive species

Mai-Uyen Nguyen,Jahangir Iqbal,Sarah Potgieter,Winston Huang,Julie Pfeffer,Sean Woo,Caifeng Zhao,Matthew Lawlor,Richard Yang,Rahma Rizly,Angela Halstead,Sharon Dent,José B Sáenz,Haiyan Zheng,Zuo-Fei Yuan,Simone Sidoli,Christopher E Ellison,Michael P Verzi

Andrology 11:1472-1483 PubMed36869699

2023

Effect of near-infrared laser treatment on improving erectile function in rats with diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Yang,Guoxiong Liu,Dali Jiang,Guiting Lin,Zejun Ren,Hengtong Fan,Bing Yang,Liyue Mu,Tom F Lue,Dalin He

BMC medicine 21:56 PubMed36922820

2023

Exogenous mitochondrial transplantation improves survival and neurological outcomes after resuscitation from cardiac arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Kei Hayashida,Ryosuke Takegawa,Yusuke Endo,Tai Yin,Rishabh C Choudhary,Tomoaki Aoki,Mitsuaki Nishikimi,Atsushi Murao,Eriko Nakamura,Muhammad Shoaib,Cyrus Kuschner,Santiago J Miyara,Junhwan Kim,Koichiro Shinozaki,Ping Wang,Lance B Becker

Scientific reports 12:21113 PubMed36477472

2022

Aporphine and isoquinoline derivatives block glioblastoma cell stemness and enhance temozolomide cytotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Dorival Mendes Rodrigues-Junior,Cristiano Raminelli,Haifa Hassanie,Gustavo Henrique Goulart Trossini,Givago Prado Perecim,Laia Caja,Aristidis Moustakas,André Luiz Vettore

Frontiers in plant science 12:727596 PubMed34646287

2021

Melatonin Alleviates Low-Temperature Stress ABI5-Mediated Signals During Seed Germination in Rice ( L.).

Applications

Unspecified application

Species

Unspecified reactive species

Ruiqing Li,Meng Jiang,Yue Song,Huali Zhang

Scientific reports 11:16359 PubMed34381166

2021

Ketogenesis controls mitochondrial gene expression and rescues mitochondrial bioenergetics after cervical spinal cord injury in rats.

Applications

Unspecified application

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Unspecified reactive species

Oscar Seira,Kathleen Kolehmainen,Jie Liu,Femke Streijger,Anne Haegert,Stéphane Lebihan,Robert Boushel,Wolfram Tetzlaff

Nature biotechnology 39:357-367 PubMed33077961

2020

Mitochondrial copper depletion suppresses triple-negative breast cancer in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Liyang Cui,Arvin M Gouw,Edward L LaGory,Shenghao Guo,Nabeel Attarwala,Yao Tang,Ji Qi,Yun-Sheng Chen,Zhou Gao,Kerriann M Casey,Arkadiy A Bazhin,Min Chen,Leeann Hu,Jinghang Xie,Mingxi Fang,Cissy Zhang,Qihua Zhu,Zhiyuan Wang,Amato J Giaccia,Sanjiv Sam Gambhir,Weiping Zhu,Dean W Felsher,Mark D Pegram,Elena A Goun,Anne Le,Jianghong Rao

Nature communications 11:2778 PubMed32513979

2020

Marriage of black phosphorus and Cu as effective photothermal agents for PET-guided combination cancer therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Kuan Hu,Lin Xie,Yiding Zhang,Masayuki Hanyu,Zhimin Yang,Kotaro Nagatsu,Hisashi Suzuki,Jiang Ouyang,Xiaoyuan Ji,Junjie Wei,Hao Xu,Omid C Farokhzad,Steven H Liang,Lu Wang,Wei Tao,Ming-Rong Zhang
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