Cytochrome C Oxidase Activity Assay Kit (ab239711) is simple, fast and high-throughput adaptable.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Andrology 11:1472-14832023Effect of near-infrared laser treatment on improving erectile function in rats with diabetes mellitus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Yang,Guoxiong Liu,Dali Jiang,Guiting Lin,Zejun Ren,Hengtong Fan,Bing Yang,Liyue Mu,Tom F Lue,Dalin HePubMed 36869699
- BMC medicine 21:562023Exogenous mitochondrial transplantation improves survival and neurological outcomes after resuscitation from cardiac arrest.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKei Hayashida,Ryosuke Takegawa,Yusuke Endo,Tai Yin,Rishabh C Choudhary,Tomoaki Aoki,Mitsuaki Nishikimi,Atsushi Murao,Eriko Nakamura,Muhammad Shoaib,Cyrus Kuschner,Santiago J Miyara,Junhwan Kim,Koichiro Shinozaki,Ping Wang,Lance B BeckerPubMed 36922820
- Scientific reports 12:211132022Aporphine and isoquinoline derivatives block glioblastoma cell stemness and enhance temozolomide cytotoxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDorival Mendes Rodrigues-Junior,Cristiano Raminelli,Haifa Hassanie,Gustavo Henrique Goulart Trossini,Givago Prado Perecim,Laia Caja,Aristidis Moustakas,André Luiz VettorePubMed 36477472
- Frontiers in plant science 12:7275962021Melatonin Alleviates Low-Temperature Stress ABI5-Mediated Signals During Seed Germination in Rice ( L.).Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuiqing Li,Meng Jiang,Yue Song,Huali ZhangPubMed 34646287
- Scientific reports 11:163592021Ketogenesis controls mitochondrial gene expression and rescues mitochondrial bioenergetics after cervical spinal cord injury in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOscar Seira,Kathleen Kolehmainen,Jie Liu,Femke Streijger,Anne Haegert,Stéphane Lebihan,Robert Boushel,Wolfram TetzlaffPubMed 34381166
- Nature biotechnology 39:357-3672020Mitochondrial copper depletion suppresses triple-negative breast cancer in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiyang Cui,Arvin M Gouw,Edward L LaGory,Shenghao Guo,Nabeel Attarwala,Yao Tang,Ji Qi,Yun-Sheng Chen,Zhou Gao,Kerriann M Casey,Arkadiy A Bazhin,Min Chen,Leeann Hu,Jinghang Xie,Mingxi Fang,Cissy Zhang,Qihua Zhu,Zhiyuan Wang,Amato J Giaccia,Sanjiv Sam Gambhir,Weiping Zhu,Dean W Felsher,Mark D Pegram,Elena A Goun,Anne Le,Jianghong RaoPubMed 33077961
- Nature communications 11:27782020Marriage of black phosphorus and Cu as effective photothermal agents for PET-guided combination cancer therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKuan Hu et. al.PubMed 32513979
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Tissue, Cell culture supernatant, Cell Lysate
Associated Products
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Target data
Function
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Alternative names
COX4, COX4I1, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1
What's included?
Recommended products
Cytochrome C Oxidase Activity Assay Kit (ab239711) is simple, fast and high-throughput adaptable.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Tissue, Cell culture supernatant, Cell Lysate
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Cytochrome C Oxidase Activity Assay Kit (ab239711) is simple, fast and high-throughput adaptable. This assay kit can be used for purified mitochondria or tissue extracts containing mitochondria. The kit provides for the fast and simple measurement of Cytochrome c oxidase activity on a 96-well plate format. The activity of the enzyme is determined colorimetrically by following the oxidation of reduced Cytochrome c as an absorbance decrease at 550 nm.
This product is manufactured by BioVision, an Abcam company and was previously called K287 Cytochrome Oxidase Activity Colorimetric Assay Kit. K287-100 is the same size as the 100 test size of ab239711.
Range of complex IV / cytochrome c oxidase assay kits
Biochemical assay - ab239711 (this kit)
Immunocapture with biochemical assay (plate-based)*** - Complex IV Rodent Enzyme Activity Microplate Assay Kit ab109911 (rodent) and Complex IV Human Enzyme Activity Microplate Assay Kit ab109909 (human)
*** Most popular assay format
Immunocapture with biochemical assay (dipstick) - Complex IV Rodent Enzyme Activity Dipstick Assay Kit ab109878 (rodent) and Complex IV Human Enzyme Activity Dipstick Assay Kit ab109876 (human)
Immunocapture with biochemical assay and ELISA - Complex IV Human Specific Activity Microplate Assay Kit ab109910 (human)
ELISA - ab179880 (human)
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Complex IV also known as cytochrome c oxidase is an important component of the electron transport chain in mitochondria. This enzyme complex has significant mass approximately 204 kDa and operates primarily in the inner mitochondrial membrane. It catalyzes the transfer of electrons from cytochrome c to oxygen facilitating ATP generation. Complex IV is ubiquitously expressed in all tissues with higher expression in organs with high energy demands like heart and skeletal muscle. This enzyme is composed of multiple subunits encoded by both mitochondrial and nuclear DNA.
- Biological function summary
Cytochrome c oxidase plays a critical role in cellular respiration. It forms part of the larger enzyme complex which also includes Complex I II and III. These complexes work together to create a proton gradient across the inner mitochondrial membrane essential for ATP synthesis via oxidative phosphorylation. This enzyme's activity is measured through assays like the cytochrome c oxidase assay which evaluates its function in various tissues. These assays help researchers understand how effectively electrons are being transferred and protons are driven across the membrane.
- Pathways
Complex IV is integral to the oxidative phosphorylation pathway and the broader mitochondrial respiratory chain. It interacts closely with cytochrome c a small heme protein that shuttles electrons between Complex III (cytochrome c reductase) and Complex IV. Through these interactions the proton gradient is established enabling ATP synthase to convert chemical energy into usable cell energy. This process significantly impacts cellular metabolism and energy production influencing how efficiently cells function.
- Associated diseases and disorders
Complex IV dysfunction is associated with mitochondrial diseases and certain neurodegenerative disorders. For instance defects in cytochrome c oxidase lead to conditions like Leigh syndrome a severe neurological disorder. Also studies show that disruptions in the electron transport chain involving Complex IV relate closely to Alzheimer’s disease. Alterations in proteins like cytochrome c which work in tandem with Complex IV can exacerbate these conditions highlighting the importance of this target in understanding and potentially treating these diseases.
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Functional Studies - Cytochrome C Oxidase Assay Kit (ab239711)
Cytochrome Oxidase Activity.
Purified mitochondria were used to calculate a decrease in OD at 550 nm (0.625-5 μg/reaction). In Blank, no change in the OD was observed. Rate is calculated by subtracting the initial OD reading from the final OD, t1 and t2 represents linear rate of reaction.
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