D-2-Hydroxyglutarate Assay Kit (ab211070) provides a convenient method to detect D-2-Hydroxyglutarate (D2HG).
- Highly specific for D-2-Hydroxyglutarate and does not detect L-2-Hydroxyglutarate
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- Nature communications 14:67812023IDH1 mutation impairs antiviral response and potentiates oncolytic virotherapy in glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXueqin Chen,Jun Liu,Yuqin Li,Yuequan Zeng,Fang Wang,Zexiong Cheng,Hao Duan,Guopeng Pan,Shangqi Yang,Yuling Chen,Qing Li,Xi Shen,Ying Li,Zixi Qin,Jiahong Chen,Youwei Huang,Xiangyu Wang,Yuli Lu,Minfeng Shu,Yubo Zhang,Guocai Wang,Kai Li,Xi Lin,Fan Xing,Haipeng ZhangPubMed 37880243
- Cell reports. Medicine 4:1010822023Haploinsufficiency of NFKBIA reshapes the epigenome antipodal to the IDH mutation and imparts disease fate in diffuse gliomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarkus Bredel,Lluís Espinosa,Hyunsoo Kim,Denise M Scholtens,Joseph P McElroy,Rajani Rajbhandari,Wei Meng,Thomas M Kollmeyer,Tathiane M Malta,Michael A Quezada,Griffith R Harsh,Teresa Lobo-Jarne,Laura Solé,Aran Merati,Surya Nagaraja,Sindhu Nair,Jaclyn J White,Nanda K Thudi,Jessica L Fleming,Amy Webb,Atsushi Natsume,Seishi Ogawa,Ruthild G Weber,Joan Bertran,S Jaharul Haque,Bettina Hentschel,C Ryan Miller,Frank B Furnari,Timothy A Chan,Anca-Ligia Grosu,Michael Weller,Jill S Barnholtz-Sloan,Michelle Monje,Houtan Noushmehr,Robert B Jenkins,C Leland Rogers,David R MacDonald,Stephanie L Pugh,Arnab ChakravartiPubMed 37343523
- Cell death discovery 9:1822023α-KG inhibits tumor growth of diffuse large B-cell lymphoma by inducing ROS and TP53-mediated ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiqing Cai,Liemei Lv,Tiange Lu,Mengfei Ding,Zhuoya Yu,Xiaomin Chen,Xiangxiang Zhou,Xin WangPubMed 37308557
- Biosensors 12:2022An Enzymatic Biosensor for the Detection of D-2-Hydroxyglutaric Acid in Serum and Urine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Wu,Zehua Li,Zepeng Kang,Chunling Ma,Haiyan Song,Fuping Lu,Zhiguang ZhuPubMed 35200327
- Journal of neuro-oncology 154:159-1702021IDH1 mutant glioma is preferentially sensitive to the HDAC inhibitor panobinostat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas K Sears,Craig M Horbinski,Kevin D WoolardPubMed 34424450
- Clinical cancer research : an official journal of the American Association for Cancer Research 27:6543-65582021Fresh Tissue Multi-omics Profiling Reveals Immune Classification and Suggests Immunotherapy Candidates for Conventional Chondrosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBinghao Li,Guoqi Li,Xiaobo Yan,Dan Zhu,Patrick P Lin,Zenan Wang,Hao Qu,Xuexin He,Yanbiao Fu,Xiuliang Zhu,Peng Lin,Jiangnan Zhang,Xiaoya Li,Hui Dai,Huabiao Chen,Mark C Poznansky,Nong Lin,Zhaoming YePubMed 34426437
- Biochemistry 60:1983-19942021Low pH Facilitates Heterodimerization of Mutant Isocitrate Dehydrogenase IDH1-R132H and Promotes Production of 2-Hydroxyglutarate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRae Sesanto,Jessamine F Kuehn,Diane L Barber,Katharine A WhitePubMed 34143606
- Cancer cell 38:334-349.e92020Integrated Metabolic and Epigenomic Reprograming by H3K27M Mutations in Diffuse Intrinsic Pontine Gliomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChan Chung et. al.PubMed 32795401
- Cancers 12:2020The Landscape of the Anti-Kinase Activity of the IDH1 Inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatarzyna Malarz et. al.PubMed 32110969
- Molecular metabolism 36:1009662020Wild-type IDH2 contributes to Epstein-Barr virus-dependent metabolic alterations and tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Shi,Ya He,Jiangjiang Li,Min Tang,Yueshuo Li,Longlong Xie,Lin Zhao,Jianmin Hu,Xiangjian Luo,Min Zhou,Na Liu,Jia Fan,Jian Zhou,Qiang Gao,ShuangJian Qiu,Weizhong Wu,Xin Zhang,Weihua Jia,Ann M Bode,Ya CaoPubMed 32224436
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Urine, Plasma, Serum, Cell Lysate
- Assay type
- Quantitative
- Reactive species
- Mammals
- Sensitivity
- = 10 µM
What's included?
Recommended products
D-2-Hydroxyglutarate Assay Kit (ab211070) provides a convenient method to detect D-2-Hydroxyglutarate (D2HG).
- Highly specific for D-2-Hydroxyglutarate and does not detect L-2-Hydroxyglutarate
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Urine, Plasma, Serum, Cell Lysate
- Assay type
- Quantitative
- Reactive species
- Mammals
- Assay Platform
- Microplate reader
- Sensitivity
- = 10 µM
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
D-2-Hydroxyglutarate Assay Kit (ab211070) provides a convenient method to detect D-2-Hydroxyglutarate (D2HG).
How the assay works
In the D 2 Hydroxyglutarate assay, D-2-Hydroxyglutarate is oxidized to alpha-Ketoglutarate in the presence of D2HG Enzyme and Substrate Mix. The intermediate reduces the probe to a colored product with strong absorbance at OD 450 nm. This absorbance is proportional to the amount of D2HG present in the samples.
D 2 Hydroxyglutarate assay protocol summary
- Add samples and standards to wells
- Add reaction mix
- Incubate for 1 hour at 37°C
- Analyze with a microplate reader
How other researchers are using ab211070
D 2 Hydroxyglutarate Assay Kit (Colorimetric) has been used in a variety of sample types including:
- Human glioma samples 1
- Primary human astrocytes 2
- Human Diffuse large B cell lymphoma 3
References: 1-Chen X al. 2023, 2-Bredel M et al. 2023, 3-Cai Y et al. 2023
Other Notes
This product was previously called K213 Biovision D-2-Hydroxyglutarate (D2HG) Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.
In eukaryotic cells, Isocitrate Dehydrogenase (IDH1, IDH2 and IDH3) catalyzes the interconversion of Isocitrate and alpha-Ketoglutarate. In human cancers, an IDH mutation causes a gain-of-function, which reduces its affinity for isocitrate and facilitates the conversion of alpha ketoglutarate to D-2-Hydroxyglutarate in the presence of NADP. D-2-Hydroxyglutarate (D2HG, D-2-Hydroxyglutarate) is present at low level in normal cells and tissues, but is significantly elevated in metabolic diseases and various cancers. Therefore, detection of elevated D2HG is important for early diagnosis, prognosis and the development of therapeutic strategies against these maladies.
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2 product images
Functional Studies - D 2 Hydroxyglutarate Assay Kit (Colorimetric) (ab211070)
Measurement of D-2-Hydroxyglutarate in different cell lysates: 3T3 (80 μg; mouse), Jurkat (12 μg), HeLa (15 μg), MDA-MB-468 (6 μg), and HepG2 (2 μg).
Functional Studies - D 2 Hydroxyglutarate Assay Kit (Colorimetric) (ab211070)
Measurement of D-2-Hydroxyglutarate in rat liver lysate (120 μg) and rat kidney lysate (240 μg).
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