D 2 Hydroxyglutarate Assay Kit (Colorimetric)

D-2-Hydroxyglutarate Assay Kit (ab211070) provides a convenient method to detect D-2-Hydroxyglutarate (D2HG) in urine, cell and tissue lysates. In this assay, D-2-Hydroxyglutarate is oxidized to α-Ketoglutarate in the presence of D2HG Enzyme and Substrate Mix. The intermediate reduces the probe to a colored product with strong absorbance at OD 450 nm. This absorbance is proportional to the amount of D2HG present in the samples. This assay kit is fast, sensitive, easy to use and high-through adaptable. It can measure D-2-Hydroxyglutarate levels less than 10 μM in various samples. This product is specific for D-2-Hydroxyglutarate and does not detect L-2-Hydroxyglutarate.

This product is manufactured by BioVision, an Abcam company and was previously called K213 D-2-Hydroxyglutarate (D2HG) Assay Kit (Colorimetric). K213-100 is the same size as the 100 test size of ab211070.

In eukaryotic cells, Isocitrate Dehydrogenase (IDH1, IDH2 and IDH3) catalyzes the interconversion of Isocitrate and α-Ketoglutarate. In human cancers, an IDH mutation causes a gain-of-function, which reduces its affinity for isocitrate and facilitates the conversion of αketoglutarate to D-2-Hydroxyglutarate in the presence of NADP. D-2-Hydroxyglutarate (D2HG, D-2-Hydroxyglutarate) is present at low level in normal cells and tissues, but is significantly elevated in metabolic diseases and various cancers. Therefore, detection of elevated D2HG is important for early diagnosis, prognosis and the development of therapeutic strategies against these maladies.