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D-Gluconate Assay Kit (Colorimetric) (ab204703) is a sensitive, fast and easy-to-use kit.

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Images

Functional Studies - D-Gluconate Assay Kit (Colorimetric) (AB204703), expandable thumbnail
  • Functional Studies - D-Gluconate Assay Kit (Colorimetric) (AB204703), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Other biological fluids, Adherent cells

Assay type

Quantitative

Target data

What's included?

100 Test
Components
ATP II
1 x 1 Vial
Assay Buffer XXII
1 x 25 mL
Developer Solution III
1 x 1 Vial
Development Enzyme Mix IX
1 x 1 Vial
Enzyme Mix XVI
1 x 1 Vial
Gluconate Converter Mix
1 x 1 Vial
Gluconate Standard
1 x 1 Vial

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D-Gluconate Assay Kit (Colorimetric) (ab204703) is a sensitive, fast and easy-to-use kit.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Other biological fluids, Adherent cells

Assay type

Quantitative

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

D-Gluconate Assay Kit (Colorimetric) (ab204703) is a sensitive, fast and easy-to-use kit. In this assay, Gluconate is utilized by Gluconokinase to form D-Gluconate-6-P and ADP, which subsequently undergoes a series of reactions to form an intermediate that reduces Gluconate probe to give a product with strong absorbance at 450 nm. This assay kit can detect D-Gluconate (D-Gluconic Acid) level less than 2 μM in a variety of samples.

This product is manufactured by BioVision, an Abcam company and was previously called K683 D-Gluconate (D-Gluconic Acid) Assay Kit (Colorimetric). K683-100 is the same size as the 100 test size of ab204703.

D-Gluconic acid (C6H12O7) is a mild organic acid that is produced from glucose by glucose oxidase. It is abundantly present in plants, fruits and animal tissues. D-Gluconate (C6H11O7) is the salt or ester of Gluconic Acid. Due to its low toxicity, it is widely used in pharmaceutical, food, and other industries.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - D-Gluconate Assay Kit (Colorimetric) (ab204703), expandable thumbnail

    Functional Studies - D-Gluconate Assay Kit (Colorimetric) (ab204703)

    Typical Gluconate Standard calibration curve using D-Gluconate Assay Kit (Colorimetric) (ab204703).

  • Functional Studies - D-Gluconate Assay Kit (Colorimetric) (ab204703), expandable thumbnail

    Functional Studies - D-Gluconate Assay Kit (Colorimetric) (ab204703)

    Measurement of Gluconate in pork muscle lysate (40 μg) and red wine (4 μL). Muscle lysate was deproteinized and PVPP was used to decolorize the red wine.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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