D-Lactate Assay Kit (Colorimetric) Kit ab83429 provides a fast, easy way to accurately measure D-lactate in a variety of biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Colorimetric
Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
Quantitative
0.01 - 10 mM
40m
> 0.01 mM
D-Lactate Assay Kit (Colorimetric) Kit ab83429 provides a fast, easy way to accurately measure D-lactate in a variety of biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Colorimetric
Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
Quantitative
0.01 - 10 mM
40m
Microplate reader
> 0.01 mM
Blue Ice
-20°C
-20°C
-20°C
D-Lactate Assay Kit (Colorimetric) Kit ab83429 provides a fast, easy way to accurately measure D-lactate in a variety of biological samples.
In the D-lactate assay protocol, D-lactate is specifically oxidized by D-lactate dehydrogenase and generates proportional color (λmax = 450 nm). The kit detects D-Lactate in samples such as serum, plasma, cells, culture and fermentation media.
D-lactate assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with a microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K667 D-Lactate Colorimetric Assay Kit. K667-100 is the same size as the 100 test size of ab83429.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Measurement of plasma D-lactate at 24h and 48h after ANP induction using ab83429.
To evaluate the severity of intestinal barrier dysfunction, plasma DAO and D-lactate were measured as indicators of intestinal mucosal mass and integrity which can reflect the extent of permeability and damage in the intestine.
Compared with the SO groups D-lactate significantly increased in the ANP groups at 24h and 48h (all p<0.05). The ANP48h group had significantly higher levels of plasma D-lactate than those of the ANP24h group (p<0.05, respectively).
D-Lactate measured in human serum, plasma and saliva, mouse serum and plasma and fresh yoghurt showing quantity (nmol) per ml of tested sample. Samples were diluted 3-27 fold.
Colorimetric standard curve: mean of duplicates (+/- SD) with background reads subtracted.
D-Lactate Standard Curve. The assay is performed according to the kit instruction. The assay specifically detects D-Lactate in the presence of up to 1000X L-Lactate.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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