D-Lactate Assay Kit (Colorimetric) Kit ab83429 provides a fast, easy way to accurately measure D-lactate in a variety of biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Images
Publications
- Nature communications 14:67792023Organoids transplantation attenuates intestinal ischemia/reperfusion injury in mice through L-Malic acid-mediated M2 macrophage polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFang-Ling Zhang,Zhen Hu,Yi-Fan Wang,Wen-Juan Zhang,Bo-Wei Zhou,Qi-Shun Sun,Ze-Bin Lin,Ke-Xuan LiuPubMed 37880227
- Nature communications 14:54372023Exosomal circEZH2_005, an intestinal injury biomarker, alleviates intestinal ischemia/reperfusion injury by mediating Gprc5a signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjuan Zhang,Bowei Zhou,Xiao Yang,Jin Zhao,Jingjuan Hu,Yuqi Ding,Shuteng Zhan,Yifeng Yang,Jun Chen,Fu Zhang,Bingcheng Zhao,Fan Deng,Zebin Lin,Qishun Sun,Fangling Zhang,Zhiwen Yao,Weifeng Liu,Cai Li,Ke-Xuan LiuPubMed 37673874
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Quantitative
- Range
0.01 - 10 mM
- Assay time
40m
- Sensitivity
> 0.01 mM
Target data
What's included?
Recommended products
D-Lactate Assay Kit (Colorimetric) Kit ab83429 provides a fast, easy way to accurately measure D-lactate in a variety of biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Quantitative
- Range
0.01 - 10 mM
- Assay time
40m
- Assay Platform
Microplate reader
- Sensitivity
> 0.01 mM
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
D-Lactate Assay Kit (Colorimetric) Kit ab83429 provides a fast, easy way to accurately measure D-lactate in a variety of biological samples.
In the D-lactate assay protocol, D-lactate is specifically oxidized by D-lactate dehydrogenase and generates proportional color (λmax = 450 nm). The kit detects D-Lactate in samples such as serum, plasma, cells, culture and fermentation media.
D-lactate assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with a microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K667 D-Lactate Colorimetric Assay Kit. K667-100 is the same size as the 100 test size of ab83429.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Image from Chen J et al., PLoS One. 2017;12(4):e0176583. Fig 2(A).; doi: 10.1371/journal.pone.0176583. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - D-Lactate Assay Kit (Colorimetric) (ab83429)
Measurement of plasma D-lactate at 24h and 48h after ANP induction using ab83429.
To evaluate the severity of intestinal barrier dysfunction, plasma DAO and D-lactate were measured as indicators of intestinal mucosal mass and integrity which can reflect the extent of permeability and damage in the intestine.
Compared with the SO groups D-lactate significantly increased in the ANP groups at 24h and 48h (all p<0.05). The ANP48h group had significantly higher levels of plasma D-lactate than those of the ANP24h group (p<0.05, respectively).
Functional Studies - D-Lactate Assay Kit (Colorimetric) (ab83429)
D-Lactate measured in human serum, plasma and saliva, mouse serum and plasma and fresh yoghurt showing quantity (nmol) per ml of tested sample. Samples were diluted 3-27 fold.
Functional Studies - D-Lactate Assay Kit (Colorimetric) (ab83429)
Colorimetric standard curve: mean of duplicates (+/- SD) with background reads subtracted.
Functional Studies - D-Lactate Assay Kit (Colorimetric) (ab83429)
D-Lactate Standard Curve. The assay is performed according to the kit instruction. The assay specifically detects D-Lactate in the presence of up to 1000X L-Lactate.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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