Skip to main content

D-Lactate Assay Kit (Colorimetric) Kit ab83429 provides a fast, easy way to accurately measure D-lactate in a variety of biological samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Be the first to review this product! Submit a review

Images

Functional Studies - D-Lactate Assay Kit (Colorimetric) (AB83429), expandable thumbnail
  • Functional Studies - D-Lactate Assay Kit (Colorimetric) (AB83429), expandable thumbnail
  • Functional Studies - D-Lactate Assay Kit (Colorimetric) (AB83429), expandable thumbnail
  • Functional Studies - D-Lactate Assay Kit (Colorimetric) (AB83429), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Assay type

Quantitative

Range

0.01 - 10 mM

Assay time

40m

Sensitivity

> 0.01 mM

Target data

What's included?

100 Test
Components
Assay Buffer XII
1 x 25 mL
D-Lactate Standard
1 x 100 µL
Developer Solution III
1 x 1 Vial
Enzyme Mix V
1 x 1 Vial

Recommended products

D-Lactate Assay Kit (Colorimetric) Kit ab83429 provides a fast, easy way to accurately measure D-lactate in a variety of biological samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Assay type

Quantitative

Range

0.01 - 10 mM

Assay time

40m

Assay Platform

Microplate reader

Sensitivity

> 0.01 mM

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

D-Lactate Assay Kit (Colorimetric) Kit ab83429 provides a fast, easy way to accurately measure D-lactate in a variety of biological samples.

In the D-lactate assay protocol, D-lactate is specifically oxidized by D-lactate dehydrogenase and generates proportional color (λmax = 450 nm). The kit detects D-Lactate in samples such as serum, plasma, cells, culture and fermentation media.

D-lactate assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with a microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K667 D-Lactate Colorimetric Assay Kit. K667-100 is the same size as the 100 test size of ab83429.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Functional Studies - D-Lactate Assay Kit (Colorimetric) (ab83429), expandable thumbnail
    Image from Chen J et al., PLoS One. 2017;12(4):e0176583. Fig 2(A).; doi: 10.1371/journal.pone.0176583. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Functional Studies - D-Lactate Assay Kit (Colorimetric) (ab83429)

    Measurement of plasma D-lactate at 24h and 48h after ANP induction using ab83429.

    To evaluate the severity of intestinal barrier dysfunction, plasma DAO and D-lactate were measured as indicators of intestinal mucosal mass and integrity which can reflect the extent of permeability and damage in the intestine.

    Compared with the SO groups D-lactate significantly increased in the ANP groups at 24h and 48h (all p<0.05). The ANP48h group had significantly higher levels of plasma D-lactate than those of the ANP24h group (p<0.05, respectively).

  • Functional Studies - D-Lactate Assay Kit (Colorimetric) (ab83429), expandable thumbnail

    Functional Studies - D-Lactate Assay Kit (Colorimetric) (ab83429)

    D-Lactate measured in human serum, plasma and saliva, mouse serum and plasma and fresh yoghurt showing quantity (nmol) per ml of tested sample. Samples were diluted 3-27 fold.

  • Functional Studies - D-Lactate Assay Kit (Colorimetric) (ab83429), expandable thumbnail

    Functional Studies - D-Lactate Assay Kit (Colorimetric) (ab83429)

    Colorimetric standard curve: mean of duplicates (+/- SD) with background reads subtracted.

  • Functional Studies - D-Lactate Assay Kit (Colorimetric) (ab83429), expandable thumbnail

    Functional Studies - D-Lactate Assay Kit (Colorimetric) (ab83429)

    D-Lactate Standard Curve. The assay is performed according to the kit instruction. The assay specifically detects D-Lactate in the presence of up to 1000X L-Lactate.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com