D-Lactate Assay Kit (Colorimetric)
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(25 Publications)
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
- FuncS
Unknown
Functional Studies - D-Lactate Assay Kit (Colorimetric) (AB83429)
D-Lactate measured in human serum, plasma and saliva, mouse serum and plasma and fresh yoghurt showing quantity (nmol) per ml of tested sample. Samples were diluted 3-27 fold.
- FuncS
Supplier Data
Functional Studies - D-Lactate Assay Kit (Colorimetric) (AB83429)
D-Lactate Standard Curve. The assay is performed according to the kit instruction. The assay specifically detects D-Lactate in the presence of up to 1000X L-Lactate.
- FuncS
Lab
Functional Studies - D-Lactate Assay Kit (Colorimetric) (AB83429)
Colorimetric standard curve : mean of duplicates (+/- SD) with background reads subtracted.
- FuncS
PubMed
Functional Studies - D-Lactate Assay Kit (Colorimetric) (AB83429)
Measurement of plasma D-lactate at 24h and 48h after ANP induction using ab83429.
To evaluate the severity of intestinal barrier dysfunction, plasma DAO and D-lactate were measured as indicators of intestinal mucosal mass and integrity which can reflect the extent of permeability and damage in the intestine.
Compared with the SO groups D-lactate significantly increased in the ANP groups at 24h and 48h (all p<0.05). The ANP48h group had significantly higher levels of plasma D-lactate than those of the ANP24h group (p<0.05, respectively).
Image from Chen J et al., PLoS One. 2017;12(4):e0176583. Fig 2(A).; doi: 10.1371/journal.pone.0176583. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
- Other
Supplier Data
Other - D-Lactate Assay Kit (Colorimetric) (AB83429)
Representative image of D-Lactate Assay Kit (Colorimetric) ab83429
Components shown from left to right :
- D-Lactate Standard
- Developer Solution III
- Enzyme Mix V
- Assay Buffer 12
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.
Product details
D-Lactate Assay Kit (Colorimetric) Kit ab83429 provides a fast, easy way to accurately measure D-lactate in a variety of biological samples.
In the D-lactate assay protocol, D-lactate is specifically oxidized by D-lactate dehydrogenase and generates proportional color (λmax = 450 nm). The kit detects D-Lactate in samples such as serum, plasma, cells, culture and fermentation media.
D-lactate assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with a microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K667 D-Lactate Colorimetric Assay Kit. K667-100 is the same size as the 100 test size of ab83429.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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Publications (25)
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BMC veterinary research 20:373 PubMed39164760
2024
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Oncology reports 51: PubMed38577936
2024
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Cellular and molecular life sciences : CMLS 81:32 PubMed38214780
2024
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Nature communications 14:6779 PubMed37880227
2023
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Nature communications 14:5437 PubMed37673874
2023
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Animal nutrition (Zhongguo xu mu shou yi xue hui) 14:130-140 PubMed37397354
2023
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Journal of translational medicine 21:264 PubMed37072757
2023
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Oncology reports 49: PubMed36960858
2023
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Annals of translational medicine 11:34 PubMed36819593
2023
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American journal of physiology. Lung cellular and molecular physiology 324:L468-L479 PubMed36809074
2023
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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