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AB83429

D-Lactate Assay Kit (Colorimetric)

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(25 Publications)

D-Lactate Assay Kit (Colorimetric) Kit ab83429 provides a fast, easy way to accurately measure D-lactate in a variety of biological samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
5 Images
Functional Studies - D-Lactate Assay Kit (Colorimetric) (AB83429)
  • FuncS

Unknown

Functional Studies - D-Lactate Assay Kit (Colorimetric) (AB83429)

D-Lactate measured in human serum, plasma and saliva, mouse serum and plasma and fresh yoghurt showing quantity (nmol) per ml of tested sample. Samples were diluted 3-27 fold.

Functional Studies - D-Lactate Assay Kit (Colorimetric) (AB83429)
  • FuncS

Supplier Data

Functional Studies - D-Lactate Assay Kit (Colorimetric) (AB83429)

D-Lactate Standard Curve. The assay is performed according to the kit instruction. The assay specifically detects D-Lactate in the presence of up to 1000X L-Lactate.

Functional Studies - D-Lactate Assay Kit (Colorimetric) (AB83429)
  • FuncS

Lab

Functional Studies - D-Lactate Assay Kit (Colorimetric) (AB83429)

Colorimetric standard curve : mean of duplicates (+/- SD) with background reads subtracted.

Functional Studies - D-Lactate Assay Kit (Colorimetric) (AB83429)
  • FuncS

PubMed

Functional Studies - D-Lactate Assay Kit (Colorimetric) (AB83429)

Measurement of plasma D-lactate at 24h and 48h after ANP induction using ab83429.

To evaluate the severity of intestinal barrier dysfunction, plasma DAO and D-lactate were measured as indicators of intestinal mucosal mass and integrity which can reflect the extent of permeability and damage in the intestine.

Compared with the SO groups D-lactate significantly increased in the ANP groups at 24h and 48h (all p<0.05). The ANP48h group had significantly higher levels of plasma D-lactate than those of the ANP24h group (p<0.05, respectively).

Image from Chen J et al., PLoS One. 2017;12(4):e0176583. Fig 2(A).; doi: 10.1371/journal.pone.0176583. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Other - D-Lactate Assay Kit (Colorimetric) (AB83429)
  • Other

Supplier Data

Other - D-Lactate Assay Kit (Colorimetric) (AB83429)

Representative image of D-Lactate Assay Kit (Colorimetric) ab83429

Components shown from left to right :

- D-Lactate Standard

- Developer Solution III

- Enzyme Mix V

- Assay Buffer 12

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Results type

Quantitative

Sensitivity

> 0.01 mM

Range

0.01 - 10 mM

Assay time

40m

Assay Platform

Microplate reader

Product details

D-Lactate Assay Kit (Colorimetric) Kit ab83429 provides a fast, easy way to accurately measure D-lactate in a variety of biological samples.

In the D-lactate assay protocol, D-lactate is specifically oxidized by D-lactate dehydrogenase and generates proportional color (λmax = 450 nm). The kit detects D-Lactate in samples such as serum, plasma, cells, culture and fermentation media.

D-lactate assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with a microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K667 D-Lactate Colorimetric Assay Kit. K667-100 is the same size as the 100 test size of ab83429.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab83429-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Enzyme Mix V", "number":"AB83429-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution III", "number":"AB83429-CMP04", "productcode":"AB255098" }, { "size":"1 x 100 µL", "name":"D-Lactate Standard", "number":"AB83429-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 12", "number":"AB83429-CMP01", "productcode":"AB323945" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Publications (25)

Recent publications for all applications. Explore the full list and refine your search

BMC veterinary research 20:373 PubMed39164760

2024

Determining lactate concentrations in Korean indigenous calves and evaluating its role as a predictor for acidemia in calf diarrhea.

Applications

Unspecified application

Species

Unspecified reactive species

Youngwoo Jung,Ji-Yeong Ku,Byoungsoo Kim,Youngjun Kim,Kwang-Man Park,Jonghun Baek,DoHyeon Yu,Jinho Park

Oncology reports 51: PubMed38577936

2024

Butyrate increases methylglyoxal production through regulation of the JAK2/Stat3/Nrf2/Glo1 pathway in castration‑resistant prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Jan Hsia,Zhang-Min Lin,Taolan Zhang,Tz-Chong Chou

Cellular and molecular life sciences : CMLS 81:32 PubMed38214780

2024

Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Jin,Meixin Shi,Jing Feng,Zhengwei Zhang,Bingbing Zhao,Qingyu Li,Ligen Yu,Zhaoyang Lu

Nature communications 14:6779 PubMed37880227

2023

Organoids transplantation attenuates intestinal ischemia/reperfusion injury in mice through L-Malic acid-mediated M2 macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Fang-Ling Zhang,Zhen Hu,Yi-Fan Wang,Wen-Juan Zhang,Bo-Wei Zhou,Qi-Shun Sun,Ze-Bin Lin,Ke-Xuan Liu

Nature communications 14:5437 PubMed37673874

2023

Exosomal circEZH2_005, an intestinal injury biomarker, alleviates intestinal ischemia/reperfusion injury by mediating Gprc5a signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjuan Zhang,Bowei Zhou,Xiao Yang,Jin Zhao,Jingjuan Hu,Yuqi Ding,Shuteng Zhan,Yifeng Yang,Jun Chen,Fu Zhang,Bingcheng Zhao,Fan Deng,Zebin Lin,Qishun Sun,Fangling Zhang,Zhiwen Yao,Weifeng Liu,Cai Li,Ke-Xuan Liu

Animal nutrition (Zhongguo xu mu shou yi xue hui) 14:130-140 PubMed37397354

2023

Acid tolerance of lactate-utilizing bacteria of the order Bacteroidales contributes to prevention of ruminal acidosis in goats adapted to a high-concentrate diet.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyan Lu,Lingmeng Kong,Shenhao Ren,Jörg R Aschenbach,Hong Shen

Journal of translational medicine 21:264 PubMed37072757

2023

Microbiota-derived tryptophan metabolites indole-3-lactic acid is associated with intestinal ischemia/reperfusion injury via positive regulation of YAP and Nrf2.

Applications

Unspecified application

Species

Unspecified reactive species

Fang-Ling Zhang,Xiao-Wei Chen,Yi-Fan Wang,Zhen Hu,Wen-Juan Zhang,Bo-Wei Zhou,Peng-Fei Ci,Ke-Xuan Liu

Oncology reports 49: PubMed36960858

2023

High‑risk pathological subtype associated FAM83A‑AS1 promotes malignancy and glycolysis of lung adenocarcinoma via miR‑202‑3p/HK2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xinkui Xiong,Lei Zhang,Bao Zang,Dafu Xu,Chen Chen,Gaochao Dong,Wenjie Xia,Yanhu Wu

Annals of translational medicine 11:34 PubMed36819593

2023

MicroRNA-155-5p inhibition alleviates irritable bowel syndrome by increasing claudin-1 and ZO-1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Guo Guo,Yue-Feng Rao,Jun Jiang,Xin Li,Sheng-Mei Zhu

American journal of physiology. Lung cellular and molecular physiology 324:L468-L479 PubMed36809074

2023

E-cigarette aerosols of propylene glycol impair BK channel activity and parameters of mucociliary function.

Applications

Unspecified application

Species

Unspecified reactive species

Michael D Kim,Samuel Chung,Nathalie Baumlin,Liang Sun,Neerupma Silswal,John S Dennis,Makoto Yoshida,Juan Sabater,Frank T Horrigan,Matthias Salathe
View all publications
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