D-Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric)

D-Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab324122) is a fluorescence-based assay for detecting D-lactate dehydrogenase (D-LDH) activity in biological samples such as serum, plasma, urine, and cell culture samples.

D-LDH assay principle
In the D-Lactate dehydrogenase assay, D-LDH acts on a substrate that is specific for lactate dehydrogenases that act on D-lactate. NADH produced by the action of the D-LDH enzyme, then reacts with a fluorogenic sensor. The amount of fluoresence produced is proportional to the amount of D-LDH activity in the sample.

This assay will detect as little as 1 mU/mL D-lactate dehydrogenase in a 100 μL reaction volume.

D-Lactate dehydrogenase assay protocol summary
- add samples and standards to wells
- add working solution and incubate for 30-min to 2-hrs at room temperature
- readout on fluorometric (Ex/Em 540/590 nm) plate reader

Other Notes
Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyzes the interconversion of pyruvate and lactate. LDH is present in cytosol of a wide variety of organisms, including animals and plants. Cells release LDH into the bloodstream after tissue damage or red blood cell hemolysis. Since LDH is a fairly stable enzyme, it has been widely used to evaluate the presence of damage and toxicity of tissue and cells. Quantification of LDH has a broad range of applications. LDH is also elevated in certain pathological conditions such as cancer.