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DAPI Staining Solution (ab228549) is a fluorescent stain for labeling DNA in fluorescence microscopy.

DAPI Staining Solution designed for success:
- Fast protocol with ready-to-use solution
- Can be used to stain live cells and fixed cells since DAPI passes through an intact cell membrane
- Cited in > 180 publications


Images

Chemical Structure - DAPI Staining Solution (AB228549), expandable thumbnail
  • Spectrum - DAPI Staining Solution (AB228549), expandable thumbnail

Publications

Key facts

Sample types

Suspension cells, Adherent cells

Associated Products

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3 products for Alternative Product

What's included?

2 mL
Components
DAPI (10 mM solution in water)
1 x 2 mL

Recommended products

DAPI Staining Solution (ab228549) is a fluorescent stain for labeling DNA in fluorescence microscopy.

DAPI Staining Solution designed for success:
- Fast protocol with ready-to-use solution
- Can be used to stain live cells and fixed cells since DAPI passes through an intact cell membrane
- Cited in > 180 publications

Key facts

Sample types

Suspension cells, Adherent cells

Assay Platform

Fluorescence microscope

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

DAPI Staining Solution (ab228549) is a fluorescent stain for labeling DNA in fluorescence microscopy. Since DAPI passes through an intact cell membrane, it can be used to stain live cells and fixed cells.

Molecular Weight: 350.25

DAPI's absorption maximum when bound to double-stranded DNA is at 358 nm and its emission maximum is at 461 nm. Although is not as strongly fluorescent as when bound to DNA, it also binds to RNA, shifting its emission to around 500 nm.

DAPI's blue emission is convenient for multiplexing assays since there is very little fluorescence overlap between DAPI and green-fluorescent molecules like FITC and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Chemical Structure - DAPI Staining Solution (ab228549), expandable thumbnail

    Chemical Structure - DAPI Staining Solution (ab228549)

    Chemical structure of DAPI.

  • Spectrum - DAPI Staining Solution (ab228549), expandable thumbnail

    Spectrum - DAPI Staining Solution (ab228549)

    Spectrum of DAPI.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com