AB228549

DAPI Staining Solution

Name: DAPI Staining Solution. Nuclear DNA stain. (ab228549) | Abcam
Brand: Abcam Limited
SKU: AB228549
Price: 145 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(260 Publications)

DAPI Staining Solution (ab228549) is a ready-to-use solution for labeling DNA in fluorescence microscopy.

- Stains both live cells and fixed cells: DAPI passes through an intact cell membrane
- Can also be used to detect mycoplasma or virus DNA in cell cultures
- Cited in over 180 publications

2 Images

Spectrum - DAPI Staining Solution (AB228549)

Spectrum

Supplier Data

Spectrum - DAPI Staining Solution (AB228549)

Spectrum of DAPI.

Chemical Structure - DAPI Staining Solution (AB228549)

Chemical Structure

Supplier Data

Chemical Structure - DAPI Staining Solution (AB228549)

Chemical structure of DAPI.

Key facts

Sample types

Suspension cells, Adherent cells

Assay Platform

Fluorescence microscope

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Product details

How the assay works

DAPI's absorption maximum when bound to double-stranded DNA is at 358 nm and its emission maximum is at 461 nm. Although is not as strongly fluorescent as when bound to DNA, it also binds to RNA, shifting its emission to around 500 nm.
Molecular Weight: 350.25
Purity: ≥95% by HPLC
DAPI's blue emission is convenient for multiplexing assays since there is very little fluorescence overlap between DAPI and green-fluorescent molecules like FITC and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red.
Besides labeling cell nuclei, DAPI is also used for the detection of mycoplasma or virus DNA in cell cultures.

DAPI Staining Solution Protocol Summary

- Pellet cells by centrifugation
- Resuspend cells in buffered salt solutions or media
- Add DAPI stain
- Incubate the sample for 15 to 60 minutes

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What's included?

{ "values": { "2mL": { "sellingSize": "2 mL", "publicAssetCode":"ab228549-2mL", "assetComponentDetails": [ { "size":"1 x 2 mL", "name":"DAPI (10 mM solution in water)", "number":"AB228549-CMP01", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

See full target information DAPI

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Publications (260)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 44:194 PubMed40616092

2025

Deciphering the tumor immune microenvironment: single-cell and spatial transcriptomic insights into cervical cancer fibroblasts.

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Zhiheng Lin,Youwei Zhou,Zhenran Liu,Wenyang Nie,Hengjie Cao,Shengnan Li,Xuanling Li,Lijun Zhu,Guangyao Lin,Yanyu Ding,Yi Jiang,Zuxi Gu,Lianwei Xu,Zhijie Zhao,Huabao Cai

European journal of medical research 30:553 PubMed40604953

2025

Intermediate cell states in spermatogonial stem cell differentiation: enhancing neurogenesis and angiogenesis in spinal cord injury.

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Xinyu Guo,Hao Yang,Haihong Zhang

Journal of translational medicine 23:631 PubMed40474178

2025

Polystyrene nanoplastics trigger pyroptosis in dopaminergic neurons through TSC2/TFEB-mediated disruption of autophagosome-lysosome fusion in Parkinson's disease.

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Xiaomei Liang,Yaqi Zeng,Piao Zhang,Baoyu Zhu,Jiezhu Feng,Tongtong Deng,Zhongling Fu,Chengshuai Liu,Chengyu Chen,Yuhu Zhang

Bioengineering & translational medicine 10:e10741 PubMed40385549

2025

Establishment of a chemoresistant laryngeal cancer cell model to study chemoresistance and chemosensitization responses via transcriptomic analysis and a tumor-on-a-chip platform.

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Christian R Moya-Garcia,Meghana Munipalle,Alain Pacis,Nader Sadeghi,Maryam Tabrizian,Nicole Y K Li-Jessen

Frontiers in neuroscience 19:1558581 PubMed40376611

2025

An evaluation of distinct adeno-associated virus vector strategies for driving transgene expression in spinal inhibitory neurons of the rat.

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Laura Klinger,Anna S M Siegert,Raphael Holzinger,Lidia Trofimova,Sibel Ada,Ruth Drdla-Schutting

Journal of tissue engineering 16:20417314251326668 PubMed40342587

2025

Development of a reliable method for human triple-negative breast cancer organotypic culture: Improving imaging and genomic studies in 3D cultures.

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Mercedes Olvera-Valencia,Verónica Garcia-Castillo,Rosalío Ramos-Payan,Maribel Aguilar-Medina,Samuel Trujano-Camacho,Alejandro López-Saavedra,Laurence A Marchat,Cesar López-Camarillo,Ronen Sumagin,Eloy Pérez-Yepez,Carlos Pérez-Plasencia

Nature communications 16:4190 PubMed40328784

2025

Astrocytic GLUT1 deletion in adult mice enhances glucose metabolism and resilience to stroke.

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Laetitia Thieren,Henri S Zanker,Jeanne Droux,Urvashi Dalvi,Matthias T Wyss,Rebecca Waag,Pierre-Luc Germain,Lukas M von Ziegler,Zoe J Looser,Ladina Hösli,Luca Ravotto,E Dale Abel,Johannes Bohacek,Susanne Wegener,L Felipe Barros,Mohamad El Amki,Bruno Weber,Aiman S Saab

Acta neuropathologica communications 13:90 PubMed40319295

2025

Systemic IGF-1 administration prevents traumatic brain injury induced gut permeability, dysmorphia, dysbiosis, and the increased number of immature dentate granule cells.

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Lavanya Venkatasamy,Jaclyn Iannucci,Aleksandr Pereverzev,Jonathan Hoar,Emily Huber,Angel Ifegbo,Reagan Dominy,Yumna El-Hakim,Kathiresh Kumar Mani,Alan Dabney,Rachel Pilla,Farida Sohrabji,Lee A Shapiro

Nature communications 16:3933 PubMed40287424

2025

Nitro-oleic acid enhances mitochondrial metabolism and ameliorates heart failure with preserved ejection fraction in mice.

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Marion Müller,Torben Schubert,Cornelius Welke,Tibor Maske,Thomas Patschkowski,Elfi Donhauser,Jacqueline Heinen-Weiler,Felix-Levin Hormann,Sven Heiles,Tina Johanna Schulz,Luisa Andrea Lengenfelder,Lucia Landwehrjohann,Elisa Theres Vogt,Bernd Stratmann,Jurek Hense,Simon Lüdtke,Martina Düfer,Elena Tolstik,Johann Dierks,Kristina Lorenz,Tamino Huxohl,Jan-Christian Reil,Vasco Sequeira,Francisco Jose Schopfer,Bruce A Freeman,Volker Rudolph,Uwe Schlomann,Anna Klinke

Nature protocols : PubMed40281336

2025

Single-nucleus total RNA sequencing of formalin-fixed paraffin-embedded samples using snRandom-seq.

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Ziye Xu,Yuexiao Lyu,Haide Chen,Yu Chen,Yongcheng Wang

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

DAPI Staining Solution

Spectrum - DAPI Staining Solution (AB228549)

Chemical Structure - DAPI Staining Solution (AB228549)

Key facts

Sample types

Assay Platform

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (260)

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Primary Antibodies

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Product promise