DCF ROS/RNS Assay Kit (biofluids, culture supernatant, cell lysates) (ab238535) is an assay for measuring the total free radical presence of a sample.
DCF ROS/RNS Assay Kit (biofluids, culture supernatant, cell lysates) designed for success:
- Complete kit with standard curve for quantitation to measure total reactive oxygen species and reactive nitrogen species, including hydrogen peroxide, nitric oxide, peroxyl radical, and peroxynitrite anion
- Can be used in a variety of sample including serum, plasma, urine, cell lysates or cell culture supernatants
- High sensitivity with a detection limit of 10 pM for DCF and 40 nM for hydrogen peroxide
Fluorescent
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DCF ROS/RNS Assay Kit (biofluids, culture supernatant, cell lysates) (ab238535) is an assay for measuring the total free radical presence of a sample.
DCF ROS/RNS Assay Kit (biofluids, culture supernatant, cell lysates) designed for success:
- Complete kit with standard curve for quantitation to measure total reactive oxygen species and reactive nitrogen species, including hydrogen peroxide, nitric oxide, peroxyl radical, and peroxynitrite anion
- Can be used in a variety of sample including serum, plasma, urine, cell lysates or cell culture supernatants
- High sensitivity with a detection limit of 10 pM for DCF and 40 nM for hydrogen peroxide
Fluorescent
Microplate reader
Blue Ice
Multi
Multi
Please refer to protocols
DCF ROS/RNS Assay Kit (biofluids, culture supernatant, cell lysates) (ab238535) is an assay for measuring the total free radical presence of a sample.
The assay employs a proprietary quenched fluorogenic probe, dichlorodihydrofluorescin DiOxyQ (DCFH-DiOxyQ), which is a specific ROS/RNS probe that is based on similar chemistry to the popular 2', 7'-dichlorodihydrofluorescein diacetate. The DCFH-DiOxyQ probe is first primed with a quench removal reagent, and subsequently stabilized in the highly reactive DCFH form. In this reactive state, ROS and RNS species can react with DCFH, which is rapidly oxidized to the highly fluorescent 2', 7'-dichlorodihydrofluorescein (DCF). Fluorescence intensity is proportional to the total ROS/RNS levels within the sample. The DCFH-DiOxyQ probe can react with hydrogen peroxide (H2O2), peroxyl radical (ROO·), nitric oxide (NO), and peroxynitrite anion (ONOO-).
The kit has a detection sensitivity limit of 10 pM for DCF and 40 nM for H2O2 respectively. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.
**Related products**Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.To measure reactive oxygen species within cells, we recommend . Alternative ROS assays are available in orange (Cellular ROS Assay Kit (Orange) ab186028), red (Cellular ROS Assay Kit (Red) ab186027), and deep red (Cellular ROS Assay Kit (Deep Red) ab186029). ab238535 is used to measure ROS in biofluids, culture supernatants and cell lysates. For assays designed to differentiate ROS, superoxides, and reactive nitrogen species: to assay ROS and superoxides use ROS/Superoxide Detection Assay Kit (Cell-based) ab139476; to assay ROS, superoxides, and reactive nitrogen species use Cellular ROS/RNS Assay Kit ab139473; to assay superoxides use Mitochondrial Superoxide Assay Kit (Fluorometric) ab219943.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Mechanism of In Vitro ROS/RNS Assay.
DCF Standard Curve.
Standard Curve.
Hydrogen Peroxide Standard Curve.
Detection of Various Free Radical Species.
DCF fluorescence curves for AAPH (peroxyl radical generator), SIN-1 (peroxynitrite generator), and SNP (nitric oxide generator).
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