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AB241002

Deubiquitinase Assay Kit

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(3 Publications)

Deubiquitinase Assay Kit (ab241002) provides a straight-forward and general measure of deubiquitinase activity by utilizing a fluorescent deubiquitinase substrate.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Functional Studies - Deubiquitinase Assay Kit (AB241002)
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Supplier Data

Functional Studies - Deubiquitinase Assay Kit (AB241002)

Example of determination of DUB activity in tissue lysates.

Rat tissue samples (10 mg each) were resuspended in DUB Assay Buffer with DTT (100 µl), homogenized, and clarified by centrifugation. The DUB activities for Rat Kidney and Liver lysates, in mU/mg, were determined to be 0.76 and 3.31, respectively. Assays were performed following the kit protocol.

Functional Studies - Deubiquitinase Assay Kit (AB241002)
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Supplier Data

Functional Studies - Deubiquitinase Assay Kit (AB241002)

Time course using positive control DUB as described.

Functional Studies - Deubiquitinase Assay Kit (AB241002)
  • FuncS

Supplier Data

Functional Studies - Deubiquitinase Assay Kit (AB241002)

AMC Standard Curve

Key facts

Detection method

Fluorescent

Sample types

Cell culture extracts, Tissue Extracts

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Deubiquitinase Assay Kit (ab241002) provides a straight-forward and general measure of deubiquitinase activity by utilizing a fluorescent deubiquitinase substrate to detect activity as low as 0.25 μU with purified enzyme.

Fl-Substrate -------> Cleaved Substrate + Fluorescence

This product is manufactured by BioVision, an Abcam company and was previously called K485 Deubiquitinase Activity Assay Kit (Fluorometric). K485-100 is the same size as the 100 test size of ab241002.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab241002-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Active DUB Enzyme", "number":"AB241002-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-Well Half Area White Plate", "number":"AB241002-CMP06", "productcode":"" }, { "size":"1 x 25 µL", "name":"DUB Substrate", "number":"AB241002-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"DUB Assay Buffer", "number":"AB241002-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"AMC Standard", "number":"AB241002-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"DTT II", "number":"AB241002-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

De-ubiquitinating enzymes (DUBs) function mechanically by removing ubiquitin moieties from proteins reversing what ubiquitination does. This action regulates protein degradation localization and activity. DUBs include enzymes such as ubiquitin-specific proteases (USPs) and ubiquitin C-terminal hydrolases (UCHs). These enzymes are typically expressed in various tissues throughout the body aligning with their roles in multiple cellular processes. DUB molecular masses vary widely reflecting the diverse structures and functions within this enzyme family.
Biological function summary

De-ubiquitination regulates protein stability and function impacting processes like DNA repair cell cycle and signal transduction. DUBs may operate as part of a larger complex interfacing with other protein groups to maintain cellular equilibrium. They handle ubiquitin chains after proteins have been tagged for degradation critical for maintaining cellular proteostasis.

Pathways

De-ubiquitinating enzymes integrate into systems that oversee protein quality control and signal transduction. Key pathways include the ubiquitin-proteasome system and the endoplasmic reticulum-associated degradation (ERAD) pathway. Related proteins within these pathways include E3 ligases such as Mdm2 and substrate proteins like p53 as de-ubiquitination dictates their fates and manipulation in cellular environments.

De-ubiquitinating enzymes have roles in cancer and neurodegenerative diseases. Alterations in DUB activities link to disorders such as Parkinson's disease and various cancers through their role in protein regulation and signal pathways. For instance DUBs influence the stability of proteins like p53 in cancer and alpha-synuclein in Parkinson's disease pointing to their importance in disease progression and therapeutic targeting.
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Product protocols

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Target data

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:114 PubMed39856683

2025

A novel USP4 inhibitor that suppresses colorectal cancer stemness by promoting β-catenin and Twist1 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Fengtian Li,Yi Zhou,Xinyue Lin,Yaxin Zhang,Qingyong Hu,Enen Zhao,Huali Li,Xingyan Pan,Feng Shu,Kun Zhang,Chengmei Huang,Na Tang,Wenting Liao

Frontiers in immunology 14:1252827 PubMed37841261

2023

Modified activities of macrophages' deubiquitinating enzymes after infection.

Applications

Unspecified application

Species

Unspecified reactive species

Vera Vozandychova,Pavel Rehulka,Kamil Hercik,Petra Spidlova,Pavla Pavlik,Jaroslav Hanus,Romana Hadravova,Jiri Stulik

Molecular biology reports 48:6075-6083 PubMed34374892

2021

DJ-1 activates the noncanonical NF-κB pathway via interaction with Cezanne to inhibit the apoptosis and promote the proliferation of Ishikawa cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Zhou Zhu,Hao-Yue Liu,Xiao-Yan Zhao,Le-Jia Qiu,Ting-Ting Zhou,Xue-Ying Wang,He-Ping Chen,Zhong-Qing Xiao
View all publications
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