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Deubiquitinase Assay Kit (ab241002) provides a straight-forward and general measure of deubiquitinase activity by utilizing a fluorescent deubiquitinase substrate.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

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Functional Studies - Deubiquitinase Assay Kit (AB241002), expandable thumbnail
  • Functional Studies - Deubiquitinase Assay Kit (AB241002), expandable thumbnail
  • Functional Studies - Deubiquitinase Assay Kit (AB241002), expandable thumbnail

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Key facts

Detection method

Fluorescent

Sample types

Cell culture extracts, Tissue Extracts

What's included?

100 Test
Components
96-Well Half Area White Plate
1 x 1 Unit
AMC Standard
1 x 100 µL
Active DUB Enzyme
1 x 1 Vial
DTT II
1 x 100 µL
DUB Assay Buffer
1 x 25 mL
DUB Substrate
1 x 25 µL

Recommended products

Deubiquitinase Assay Kit (ab241002) provides a straight-forward and general measure of deubiquitinase activity by utilizing a fluorescent deubiquitinase substrate.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Key facts

Detection method

Fluorescent

Sample types

Cell culture extracts, Tissue Extracts

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Deubiquitinase Assay Kit (ab241002) provides a straight-forward and general measure of deubiquitinase activity by utilizing a fluorescent deubiquitinase substrate to detect activity as low as 0.25 μU with purified enzyme.

Fl-Substrate -------> Cleaved Substrate + Fluorescence

This product is manufactured by BioVision, an Abcam company and was previously called K485 Deubiquitinase Activity Assay Kit (Fluorometric). K485-100 is the same size as the 100 test size of ab241002.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

De-ubiquitinating enzymes (DUBs) function mechanically by removing ubiquitin moieties from proteins reversing what ubiquitination does. This action regulates protein degradation localization and activity. DUBs include enzymes such as ubiquitin-specific proteases (USPs) and ubiquitin C-terminal hydrolases (UCHs). These enzymes are typically expressed in various tissues throughout the body aligning with their roles in multiple cellular processes. DUB molecular masses vary widely reflecting the diverse structures and functions within this enzyme family.

Biological function summary

De-ubiquitination regulates protein stability and function impacting processes like DNA repair cell cycle and signal transduction. DUBs may operate as part of a larger complex interfacing with other protein groups to maintain cellular equilibrium. They handle ubiquitin chains after proteins have been tagged for degradation critical for maintaining cellular proteostasis.

Pathways

De-ubiquitinating enzymes integrate into systems that oversee protein quality control and signal transduction. Key pathways include the ubiquitin-proteasome system and the endoplasmic reticulum-associated degradation (ERAD) pathway. Related proteins within these pathways include E3 ligases such as Mdm2 and substrate proteins like p53 as de-ubiquitination dictates their fates and manipulation in cellular environments.

Associated diseases and disorders

De-ubiquitinating enzymes have roles in cancer and neurodegenerative diseases. Alterations in DUB activities link to disorders such as Parkinson's disease and various cancers through their role in protein regulation and signal pathways. For instance DUBs influence the stability of proteins like p53 in cancer and alpha-synuclein in Parkinson's disease pointing to their importance in disease progression and therapeutic targeting.

Product promise

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3 product images

  • Functional Studies - Deubiquitinase Assay Kit (ab241002), expandable thumbnail

    Functional Studies - Deubiquitinase Assay Kit (ab241002)

    AMC Standard Curve

  • Functional Studies - Deubiquitinase Assay Kit (ab241002), expandable thumbnail

    Functional Studies - Deubiquitinase Assay Kit (ab241002)

    Time course using positive control DUB as described.

  • Functional Studies - Deubiquitinase Assay Kit (ab241002), expandable thumbnail

    Functional Studies - Deubiquitinase Assay Kit (ab241002)

    Example of determination of DUB activity in tissue lysates.

    Rat tissue samples (10 mg each) were resuspended in DUB Assay Buffer with DTT (100 l), homogenized, and clarified by centrifugation. The DUB activities for Rat Kidney and Liver lysates, in mU/mg, were determined to be 0.76 and 3.31, respectively. Assays were performed following the kit protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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