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AB242293

Diacylglycerol Assay Kit

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(7 Publications)

ab242293 measures diacylglycerol (DAG) content in samples by a coupled enzymatic reaction system.
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Functional Studies - Diacylglycerol Assay Kit (AB242293)
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Supplier Data

Functional Studies - Diacylglycerol Assay Kit (AB242293)

DAG Detection of Lipid Extracts.

HEK293 and COS-7 lipid extracts were prepared according to the extraction procedure above. DAG samples were tested according to the Assay Protocol (phosphatidic acid background was determined +/- Kinase Mixture). Negative control values (without DAG) have been subtracted.

Functional Studies - Diacylglycerol Assay Kit (AB242293)
  • FuncS

Supplier Data

Functional Studies - Diacylglycerol Assay Kit (AB242293)

DAG Assay Standard Curve.

DAG standard curve was performed according to the Assay Protocol. Background has been subtracted.

Key facts

Detection method

Fluorescent

Sample types

Cell Lysate

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Product details

ab242293 measures diacylglycerol (DAG) content in samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG samples, yielding phosphatidic acid. Next, a lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Finally, the glycerol-3- phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide which reacts with the kit's Fluorometric Probe (Ex. 530-560 nm/Em. 585-595 nm).

ab242293 is a simple, fluorometric assay that quantitatively measures total DAG in cell lysates using a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards and unknown samples. The kit contains a DAG Standard and has a detection sensitivity limit of ~15 uM.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab242293-100Test", "assetComponentDetails": [ { "size":"1 x 1 mL", "name":"DAG Standard", "number":"AB242293-CMP02", "productcode":"" }, { "size":"3 x 1.4 mL", "name":"Lipase Solution", "number":"AB242293-CMP06", "productcode":"" }, { "size":"1 x 110 µL", "name":"Fluorometric Probe", "number":"AB242293-CMP04", "productcode":"" }, { "size":"3 x 1.75 mL", "name":"Enzyme Mixture", "number":"AB242293-CMP03", "productcode":"" }, { "size":"2 x 1 mL", "name":"Kinase Mixture", "number":"AB242293-CMP05", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"10X Assay Buffer", "number":"AB242293-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
-80°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Diacylglycerol (DAG) is a lipid molecule that plays a mechanical role as a secondary messenger in cellular processes. It is sometimes referred to as DAG lipid or diacylglycerols. DAG consists of two fatty acid chains bonded to a glycerol backbone. DAG is expressed in the cell membrane and cytoplasm across various tissues. The mass of this molecule varies depending on the length and saturation of its fatty acid chains which can impact its specific role and function in different biological contexts.
Biological function summary

Diacylglycerol participates in signal transduction pathways by activating protein kinase C (PKC) an important component in cellular signaling. DAG does not typically function alone; it often acts within multiprotein complexes influencing various cellular responses through modulation of PKC activity. Through these interactions DAG regulates processes like cell growth differentiation and apoptosis.

Pathways

DAG is a central component in the phosphoinositide pathway and the mitogen-activated protein kinase (MAPK) pathway. In the phosphoinositide pathway it works alongside inositol trisphosphate (IP3) to transduce extracellular signals into cellular responses. In the MAPK pathway DAG aids in the activation of proteins that play roles in proliferation and stress response. Related proteins include phospholipase C which produces DAG by hydrolyzing phosphatidylinositol 45-bisphosphate and PKC which is activated by DAG to propagate intracellular signaling.

DAG is linked to conditions such as cancer and obesity. Dysregulation of DAG levels can lead to uncontrolled cell proliferation contributing to tumorigenesis. In obesity altered DAG signaling may impair insulin sensitivity affecting glucose metabolism. In these contexts the protein kinase C family is often implicated due to DAG's role in PKC activation making DAG a target of interest in disease modulation and treatment strategies.
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Product protocols

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Target data

See full target information Diacylglycerol
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Molecular oncology 17:2314-2336 PubMed37699867

2023

Comparative membrane lipidomics of hepatocellular carcinoma cells reveals diacylglycerol and ceramide as key regulators of Wnt/β-catenin signaling and tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Yagmur Azbazdar,Yeliz Demirci,Guillaume Heger,Dogac Ipekgil,Mustafa Karabicici,Gunes Ozhan

Cell reports. Medicine 4:100880 PubMed36603576

2023

DGKB mediates radioresistance by regulating DGAT1-dependent lipotoxicity in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunkoo Kang,Haksoo Lee,Kyeongmin Kim,Eunguk Shin,Byeongsoo Kim,JiHoon Kang,Bohkyung Kim,Jung Sub Lee,Jae-Myung Lee,HyeSook Youn,BuHyun Youn

Cell metabolism 34:1960-1976.e9 PubMed36476935

2022

Lipid-laden lung mesenchymal cells foster breast cancer metastasis via metabolic reprogramming of tumor cells and natural killer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Gong,Qing Li,Jiayuan Shi,Edison T Liu,Leonard D Shultz,Guangwen Ren

Nutrition & diabetes 12:20 PubMed35418570

2022

Detrimental effects of branched-chain amino acids in glucose tolerance can be attributed to valine induced glucotoxicity in skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher A Bishop,Tina Machate,Thorsten Henning,Janin Henkel,Gerhard Püschel,Daniela Weber,Tilman Grune,Susanne Klaus,Karolin Weitkunat

Photochemistry and photobiology 97:1136-1144 PubMed33977551

2021

Melanopsin Signaling Pathway in HEK293 Cell Line with Stable Expression of Human Melanopsin: Possible Participation of Phospholipase C beta 4 and Diacylglycerol.

Applications

Unspecified application

Species

Unspecified reactive species

Olga I Krzysztynska-Kuleta,Magdalena M Olchawa,Tadeusz J Sarna

Molecular metabolism 44:101133 PubMed33271332

2020

Hepatocyte-specific PKCβ deficiency protects against high-fat diet-induced nonalcoholic hepatic steatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoling Shu,Faizule Hassan,Vincenzo Coppola,Kedryn K Baskin,Xianlin Han,Neil K Mehta,Michael C Ostrowski,Kamal D Mehta

Cell metabolism 31:909-919.e8 PubMed32275862

2020

Pimozide Alleviates Hyperglycemia in Diet-Induced Obesity by Inhibiting Skeletal Muscle Ketone Oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Rami Al Batran,Keshav Gopal,Megan E Capozzi,Jadin J Chahade,Bruno Saleme,S Amirhossein Tabatabaei-Dakhili,Amanda A Greenwell,Jingjing Niu,Malak Almutairi,Nikole J Byrne,Grant Masson,Ryekjang Kim,Farah Eaton,Erin E Mulvihill,Léa Garneau,Andrea R Masters,Zeruesenay Desta,Carlos A Velázquez-Martínez,Céline Aguer,Peter A Crawford,Gopinath Sutendra,Jonathan E Campbell,Jason R B Dyck,John R Ussher
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