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Diamine Oxidase Assay Kit (ab241004) provides a straightforward method to determine DAO activity of tissue lysates as well as recombinant enzymes, with a detection limit of less than 1 pmol/min of activity.

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Detection method

Fluorescent

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Shipping To:
United States

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What's included?

100 Test
Components
DAO Assay Buffer
1 x 25 mL
DAO Enzyme Mix (Lyophilized)
1 x 1 Vial
DAO Positive Control (Lyophilized)
1 x 1 Vial
DAO Probe (in DMSO)
1 x 200 µL
DAO Substrate (Lyophilized)
1 x 1 Vial
H2O2 Standard (0.88 M)
1 x 100 µL

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Diamine Oxidase Assay Kit (ab241004) provides a straightforward method to determine DAO activity of tissue lysates as well as recombinant enzymes, with a detection limit of less than 1 pmol/min of activity.

Key facts

Detection method

Fluorescent

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Diamine Oxidase Assay Kit (ab241004) provides a straightforward method to determine DAO activity of tissue lysates as well as recombinant enzymes, with a detection limit of less than 1 pmol/min of activity.

In the assay, DAO converts the provided substrate, yielding an intermediate and H2O2. H2O2 is then utilized by the DAO Enzyme Mix to generate fluorescence (Ex/Em = 535/587 nm) from the DAO Probe.

This product is manufactured by BioVision, an Abcam company and was previously called K496 Diamine Oxidase Activity Assay Kit (Fluorometric). K496-100 is the same size as the 100 test size of ab241004.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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