AB239705

Dihydrofolate Reductase Assay Kit (Colorimetric)

Name: Dihydrofolate Reductase Assay Kit (Colorimetric) (ab239705) | Abcam
Brand: Abcam Limited
SKU: AB239705
Price: 595 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Dihydrofolate Reductase Assay Kit (Colorimetric) (ab239705) is based on the ability of DHFR to catalyze the oxidation of NADPH.

View Alternative Names

Dihydrofolate reductase, DHFR

4 Images

Functional Studies - Dihydrofolate Reductase Assay Kit (Colorimetric) (AB239705)

FuncS

Supplier Data

Functional Studies - Dihydrofolate Reductase Assay Kit (Colorimetric) (AB239705)

Dihydrofolate Reductase specific activity was calculated from Rat Liver (100 µg) or Hela cells (80 µg)

FuncS

Supplier Data

Functional Studies - Dihydrofolate Reductase Assay Kit (Colorimetric) (AB239705)

Dihydrofolate Reductase activity in Rat Liver (100 µg)

FuncS

Supplier Data

Functional Studies - Dihydrofolate Reductase Assay Kit (Colorimetric) (AB239705)

Purified Dihydrofolate Reductase activity

FuncS

Unknown

Functional Studies - Dihydrofolate Reductase Assay Kit (Colorimetric) (AB239705)

NADPH standard curve

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Assay Platform

Microplate reader

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Dihydrofolate Reductase Assay Kit (Colorimetric) (ab239705) is based on the ability of DHFR to catalyze the oxidation of NADPH. The reaction progress is followed by monitoring the decrease in absorbance at 340 nm. Our assay has been optimized to be carried out in a 96-well plate. It is simple, sensitive and can detect as low as 4 mU/ml in a variety of samples.

Other Notes
This product was previously called K246 Biovision Dihydrofolate Reductase Activity Kit (Colorimetric). Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

style

left-column

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab239705-100Test", "assetComponentDetails": [ { "size":"1 x 10 µL", "name":"Dihydrofolate Reductase", "number":"AB239705-CMP03", "productcode":"" }, { "size":"1 x 450 µL", "name":"DHFR Substrate", "number":"AB239705-CMP02", "productcode":"" }, { "size":"1 x 35 mL", "name":"DHFR Assay Buffer", "number":"AB239705-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"NADPH III", "number":"AB239705-CMP04", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

style

left-column

style

left-column

Product protocols

style

left-column

Target data

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.

See full target information Dihydrofolate reductase

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Drug development research 86:e70085 PubMed40152211

2025

Novel 3-Substituted-2H-Chromene Scaffold Based Fluorinated Hydrophobic Fragment as In-Vitro Antiproliferative Agents and Apoptosis Inducers Targeting Both VEGFR-2/BRAF and h-DHFR With Molecular Docking Simulation.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed A Salem,Moustafa S Abusaif,Nirvana A Gohar,Yousry A Ammar,Ahmed Ragab

Journal of experimental & clinical cancer research : CR 41:53 PubMed35135603

2022

Chloride intracellular channel 1 activity is not required for glioblastoma development but its inhibition dictates glioma stem cell responsivity to novel biguanide derivatives.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Barbieri,Alessia Graziana Bosio,Alessandra Pattarozzi,Michele Tonelli,Adriana Bajetto,Ivan Verduci,Francesca Cianci,Gaetano Cannavale,Luca M G Palloni,Valeria Francesconi,Stefano Thellung,Pietro Fiaschi,Samanta Mazzetti,Silvia Schenone,Beatrice Balboni,Stefania Girotto,Paolo Malatesta,Antonio Daga,Gianluigi Zona,Michele Mazzanti,Tullio Florio

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Dihydrofolate Reductase Assay Kit (Colorimetric)

Functional Studies - Dihydrofolate Reductase Assay Kit (Colorimetric) (AB239705)

Functional Studies - Dihydrofolate Reductase Assay Kit (Colorimetric) (AB239705)

Functional Studies - Dihydrofolate Reductase Assay Kit (Colorimetric) (AB239705)

Functional Studies - Dihydrofolate Reductase Assay Kit (Colorimetric) (AB239705)

Key facts

Detection method

Sample types

Assay Platform

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise