AB204722

Dipeptidyl peptidase IV (DPP4) Activity Assay Kit (Fluorometric)

Name: Dipeptidyl peptidase IV (DPP4) Activity Assay Kit (Fluorometric) | Abcam
Brand: Abcam Limited
SKU: AB204722
Price: 575 USD
Availability: InStock

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(7 Publications)

Dipeptidyl peptidase IV (DPP4) Activity Assay Kit ab204722 is an assay where DPP4 cleaves a substrate to release a quenched fluorescent group, AMC (7-Amino-4-Methyl Coumarin), which can be easily detected at Ex/Em = 360/460 nm.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

CD26, ADCP2, DPP4, Dipeptidyl peptidase 4, ADABP, Adenosine deaminase complexing protein 2, Dipeptidyl peptidase IV, T-cell activation antigen CD26, TP103, ADCP-2, DPP IV

2 Images

Functional Studies - Dipeptidyl peptidase IV (DPP4) Activity Assay Kit (Fluorometric) (AB204722)

FuncS

Supplier Data

Functional Studies - Dipeptidyl peptidase IV (DPP4) Activity Assay Kit (Fluorometric) (AB204722)

Typical AMC Standard calibration curve.

FuncS

Supplier Data

Functional Studies - Dipeptidyl peptidase IV (DPP4) Activity Assay Kit (Fluorometric) (AB204722)

Dipeptidyl peptidase IV (DPP4) Positive Control.

Key facts

Detection method

Fluorescent

Sample types

Tissue, Suspension cells, Serum, Adherent cells

Assay type

Enzyme activity

Assay time

40m

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Dipeptidyl peptidase IV (DPP4) Activity Assay Kit (Fluorometric) (ab204722) is an assay where DPP4 cleaves a substrate to release a quenched fluorescent group, AMC (7-Amino-4-Methyl Coumarin), which can be easily detected at Ex/Em = 360/460 nm. This assay is rapid, simple, sensitive, and reliable, as well as, suitable for high throughput activity screening of DPP4. This kit detects DPP4 activity as low as 3 uU per well.

DPP4 assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader for 30 min in kinetic mode

Other Notes
This product was previously called K779 Biovision DPP4 Activity Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

Dipeptidyl peptidase-4 (DPP4), also known as adenosine deaminase complexing protein 2 or CD26 (cluster of differentiation 26) is a protein that, in humans, is encoded by the DPP4 gene. The substrates of CD26/DPP4 are proline (or alanine) containing peptides and include growth factors, chemokines, neuropeptides, and vasoactive peptides. DPP4 plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1 and hence its inhibition by drugs such as Sitagliptin have been used for treatment of diabetes mellitus type 2. DPP4 also appears to work as a suppressor in the development of cancer and tumors.

The standard is affinity purified.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab204722-100Test", "assetComponentDetails": [ { "size":"1 x 20 µL", "name":"DPP4 Positive Control", "number":"AB204722-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"DPP4 Inhibitor", "number":"AB204722-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"DPP4 Assay Buffer", "number":"AB204722-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"DPP4 Substrate", "number":"AB204722-CMP05", "productcode":"" }, { "size":"1 x 100 µL", "name":"AMC Standard", "number":"AB204722-CMP01", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dipeptidyl peptidase 4 also known as DPP4 or CD26 functions as a serine exopeptidase. This enzyme is involved in cleaving dipeptides from the N-terminus of polypeptides. DPP4 with a mass of approximately 110 kDa is expressed in various tissues such as the liver intestines kidney and lymphocytes. It exists in both a membrane-bound form and a soluble form in blood plasma.

Biological function summary

DPP4 plays a significant role in glucose metabolism and immune function. The enzyme interacts with members of the immune system and regulates proteins such as chemokines growth factors and neuropeptides. DPP4 acts independently and is not known to be part of a larger complex. It modulates biological processes by inactivating peptide hormones and signaling molecules which impacts glucose homeostasis and immune responses.

Pathways

DPP4 impacts the incretin signaling pathway and the immune response pathway. Incretin hormones like glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are substrates of DPP4 and play important roles in glucose metabolism. DPP4 also interacts with proteins such as adenosine deaminase (ADA) which impacts T-cell function and immune regulation. The regulation of these pathways by DPP4 influences metabolic and immune health.

DPP4 involvement is relevant in type 2 diabetes and immunological disorders. In type 2 diabetes DPP4 inhibitors modulate the incretin hormones improving glycemic control. Proteins like GLP-1 are affected by DPP4 activity connecting it to diabetes management. In autoimmune diseases altered DPP4 expression can impact immune cell functions influencing conditions such as rheumatoid arthritis. Through its effects on ADA and associated pathways DPP4 can influence both metabolic and immune-related disorders.

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Product protocols

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Target data

Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation (PubMed : 10900005, PubMed : 10951221, PubMed : 11772392, PubMed : 17287217). Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC (PubMed : 10900005, PubMed : 10951221, PubMed : 11772392, PubMed : 14691230). Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner (PubMed : 17287217). Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion (PubMed : 11772392). In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM (PubMed : 10593948, PubMed : 16651416). May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation (PubMed : 18708048). When overexpressed, enhanced cell proliferation, a process inhibited by GPC3 (PubMed : 17549790). Also acts as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones such as brain natriuretic peptide 32 (PubMed : 10570924, PubMed : 16254193). Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline (PubMed : 10593948).. (Microbial infection) Acts as a receptor for human coronavirus MERS-CoV-2.

See full target information DPP4

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 13:357 PubMed35883204

2022

Adipose-derived stromal cells reverse insulin resistance through inhibition of M1 expression in a type 2 diabetes mellitus mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Lee-Wei Chen,Pei-Hsuan Chen,Chia-Hua Tang,Jui-Hung Yen

International journal of molecular sciences 23: PubMed35456981

2022

Glucagon-like Peptide-1 Secretion Is Inhibited by Lysophosphatidic Acid.

Applications

Unspecified application

Species

Unspecified reactive species

Maria F Fernandes,Michelle V Tomczewski,Robin E Duncan

PloS one 16:e0252153 PubMed34043673

2021

Inhibiting adipose tissue M1 cytokine expression decreases DPP4 activity and insulin resistance in a type 2 diabetes mellitus mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Lee-Wei Chen,Pei-Hsuan Chen,Jui-Hung Yen

Nutrients 13: PubMed33808180

2021

Twelve-Week Mediterranean Diet Intervention Increases Citrus Bioflavonoid Levels and Reduces Inflammation in People with Type 2 Diabetes Mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Hayder A Al-Aubaidy,Aanchal Dayan,Myrna A Deseo,Catherine Itsiopoulos,Dina Jamil,Najah R Hadi,Colleen J Thomas

Cancer research 79:735-746 PubMed30584072

2018

Inhibition of Dipeptidyl Peptidase-4 Accelerates Epithelial-Mesenchymal Transition and Breast Cancer Metastasis via the CXCL12/CXCR4/mTOR Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yang,Yuta Takagaki,Yasuo Yoshitomi,Takayuki Ikeda,Jinpeng Li,Munehiro Kitada,Asako Kumagai,Emi Kawakita,Sen Shi,Keizo Kanasaki,Daisuke Koya

Kidney & blood pressure research 43:987-999 PubMed29913457

2018

Anti-Inflammatory Action of Sitagliptin and Linagliptin in Doxorubicin Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Chor Ho Jo,Sua Kim,Joon-Sung Park,Gheun-Ho Kim

Diabetes 67:518-531 PubMed29254987

2017

DPP-4 Inhibitors Improve Diabetic Wound Healing via Direct and Indirect Promotion of Epithelial-Mesenchymal Transition and Reduction of Scarring.

Applications

Unspecified application

Species

Unspecified reactive species

Min Long,Leiqin Cai,Wenjie Li,Linlin Zhang,Shaodong Guo,Rui Zhang,Yi Zheng,Xiufei Liu,Min Wang,Xianli Zhou,Hui Wang,Xing Li,Ling Li,Zhiming Zhu,Gangyi Yang,Hongting Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Dipeptidyl peptidase IV (DPP4) Activity Assay Kit (Fluorometric)

Functional Studies - Dipeptidyl peptidase IV (DPP4) Activity Assay Kit (Fluorometric) (AB204722)

Functional Studies - Dipeptidyl peptidase IV (DPP4) Activity Assay Kit (Fluorometric) (AB204722)

Key facts

Detection method

Sample types

Assay type

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Assay Kits

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise