DL-Serine Assay Kit

• Biochemical assay

Unknown

Biochemical assay - DL-Serine Assay Kit (AB241027)

D-Serine Standard curve.

• Biochemical assay

Unknown

Biochemical assay - DL-Serine Assay Kit (AB241027)

Estimation of total, D- and L-Serine in pooled normal human serum and CSF (10 μL).

Estimation of total, D- and L-Serine in pooled normal human serum and CSF (10 μL). L-Serine concentrations for serum and CSF samples were 137.4 ± 9.06 μM and 56.01 ± 2.93 μM, whereas D-Serine concentrations were 2.37 ± 0.36 μM and 3.46 ± 0.21 μM, respectively. Data are mean ± SEM of 3 replicates, assayed according to the kit protocol.

• Biochemical assay

Unknown

Biochemical assay - DL-Serine Assay Kit (AB241027)

Specificity for detection of L- or D-Serine (SER) over other amino acids. At a 5-fold molar excess (10 nmoles) versus SER isomers (each 2 nmoles), L-threonine (THR) contributes ≤15% interference, L-cysteine (CYS) contributes ≤10% interference and all other amino acids contribute ≤5%.

• Schematic Diagram

Lab

Schematic Diagram - DL-Serine Assay Kit (AB241027)

L-serine is isomerized by the Serine Racemase Enzyme Mix. D-serine is then converted to an intermediate by the D-Serine Enzyme Mix which ultimately reacts with the Serine Developer Mix and OxiRed Probe to produce a fluorogenic product with an excitation/emission maximum at 535/587 nm.