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DNA damage Assay Kit (AP sites, Colorimetric) ab211154 provides a sensitive and specific method to monitor the formation of apurinic/apyrimidinic (AP) sites, one of the major types of DNA lesions.

DNA Damage Assay Kit (AP sites, Colorimetric) designed for success:
- High sensitivity, of which the detection limit can be as low as 4-40 AP sites in 10^5 bp DNA
- Suitable for use with cells or tissues
- Oxidized and reduced DNA standards included for absolute quantitation

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Functional Studies - DNA Damage Assay Kit (AP sites, Colorimetric) (AB211154), expandable thumbnail

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Key facts

Detection method

Colorimetric

Sample types

DNA

Assay type

Quantitative

What's included?

50 Test
Components
10X Wash Buffer
1 x 30 mL
ARP Solution
1 x 250 µL
ARP-DNA Standard
1 x 400 µL
DNA Binding Solution
1 x 6 mL
DNA High-Binding Plate
1 x 1 Unit
Glycogen Solution
1 x 100 µL
Reduced DNA Standard
1 x 1 mL
Sodium Acetate Solution
1 x 1 mL
Stop Solution
1 x 12 mL
Streptavidin-Enzyme Conjugate
1 x 20 µL
Substrate Solution
1 x 12 mL

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DNA damage Assay Kit (AP sites, Colorimetric) ab211154 provides a sensitive and specific method to monitor the formation of apurinic/apyrimidinic (AP) sites, one of the major types of DNA lesions.

DNA Damage Assay Kit (AP sites, Colorimetric) designed for success:
- High sensitivity, of which the detection limit can be as low as 4-40 AP sites in 10^5 bp DNA
- Suitable for use with cells or tissues
- Oxidized and reduced DNA standards included for absolute quantitation

Key facts

Detection method

Colorimetric

Sample types

DNA

Assay type

Quantitative

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

DNA damage Assay Kit (AP sites, Colorimetric) (ab211154) provides a sensitive and specific method to monitor the formation of apurinic/apyrimidinic (AP) sites, one of the major types of DNA lesions.

This DNA damage assay uses an APR (Aldehyde Reactive Probe) that reacts specifically with an aldehyde group on the open ring form of AP sites. AP sites are then tagged with biotin residues that can later be quantified using an streptavidin-enzyme conjugate that is easily detected by absorbance at OD450 nm. The kit has a detection sensitivity range of 4-40 AP sites per 1 x 105 bp.

Free radicals and other reactive species are constantly generated in vivo and cause oxidative damage to biomolecules, a process held in check only by the existence of multiple antioxidant and repair systems as well as the replacement of damaged lipids and proteins. DNA is probably the most biologically significant target of oxidative attack, and it is widely thought that continuous oxidative damage to DNA is a significant contributor to the age-related development of the major cancers, such as those of the colon, breast, rectum, and prostate. Among numerous types of oxidative DNA damage, apurinic/apyrimidinic (AP or abasic) site is one of the prevalent lesions of oxidative DNA damage. Abasic sites arise in DNA at a significant rate by spontaneous base loss as in depurination, by DNA oxidation, or by the action of DNA glycosylases. Estimates of the number of abasic sites generated per mammalian cell run as high as 50,000 to 200,000 per day. Unrepaired abasic sites inhibit topoisomerases, replication, and transcription and can be mutagenic because of bypass synthesis on nontemplated DNA.

Product promise

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1 product image

  • Functional Studies - DNA Damage Assay Kit (AP sites, Colorimetric) (ab211154), expandable thumbnail

    Functional Studies - DNA Damage Assay Kit (AP sites, Colorimetric) (ab211154)

    Typical ARP-DNA standard calibration curve.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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