DNA Damage Assay Kit (AP sites, Colorimetric)

Product details

DNA damage – AP sites – Assay Kit (Colorimetric) (ab211154) is a sensitive assay for the detection of AP sites with a sensitivity of 4-40 AP sites per 10^5 bp.

How the assay works

This DNA damage assay uses an APR (Aldehyde Reactive Probe) that reacts specifically with an aldehyde group on the open ring form of AP sites. AP sites are then tagged with biotin residues that can later be quantified using an streptavidin-enzyme conjugate that is easily detected by absorbance at OD450 nm. The kit has a detection sensitivity range of 4-40 AP sites per 1 x 10^5 bp.

DNA damage assay protocol summary

- ARP-DNA Standard preparation

- DNA purification

- ARP reaction

- Incubation with DNA Binding Solution

- Incubation with Streptavidin-Enzyme conjugate

- Incubation with Substrate Solution

- Measure absorbance at OD 450 nm

How other researchers are using DNA Damage Assay Kit ab211154

ab211154 has been used in a variety of sample type including:

- Mouse liver samples (1)

- Rat liver and kidney tissues (2)

- HEK293FT cell line (3)

References:

1 - Song Y et al. 2023; 2 - Mesnage R et al. 2021; 3 - Kim D et al. 2021.

Related and recommended products

DNA damage Assay Kit requires purification of genomic DNA. We recommend using Genomic DNA Isolation Kit ab65358.

Another kit to study DNA damage is CPD ELISA Kit (cyclobutane pyrimidine dimers, DNA samples) ab242297.