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AB113472

DNA demethylase (total) Activity Quantification Assay Kit

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DNA methylation plays an essential role in biological processess through repression of transcription, while DNA demethylation is necessary, on the other hand, for epigenetic reprogramming of these genes.
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Functional Studies - DNA demethylase (total) Activity Quantification Assay Kit (AB113472)
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Supplier Data

Functional Studies - DNA demethylase (total) Activity Quantification Assay Kit (AB113472)

Nuclear extracts were prepared from MCF-7 cells using the Nuclear Extraction Kit (ab113474) and total DNA demethylase activity was measured using ab113472.

Key facts

Assay type

Enzyme activity

Assay time

3h

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

DNA methylation plays an essential role in biological processess through repression of transcription, while DNA demethylation is necessary, on the other hand, for epigenetic reprogramming of these genes. Active demethylation of DNA requires specific demethylase participation such as from MBD2.

DNA demethylase (total) Activity Quantification Assay Kit (ab113472) allows the user to measure total DNA demethylase activity/inhibition at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed for measuring total DNA demethylase activity/inhibition.

ab113472 is suitable for measuring DNA demethylase activity/inhibition from cultured cells/tissues or using purified enzyme/protein extracts.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

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