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DNA methylation plays an essential role in biological processess through repression of transcription, while DNA demethylation is necessary, on the other hand, for epigenetic reprogramming of these genes.

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Functional Studies - DNA demethylase (total) Activity Quantification Assay Kit (AB113472), expandable thumbnail

Key facts

Assay type
Enzyme activity
Reactive species
Mouse, Human
Assay time
3h

Associated Products

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What's included?

96 Test
Components
10X Wash Buffer
1 x 22 mL
8-Well Assay Strips (with Frame)
12 x 1 Unit
Capture Antibody, 1000 µg/mL
1 x 8 µL
Demethylase Assay Buffer
1 x 6 mL
Demethylase Substrate
1 x 0.2 mL
Demethylation Standard, 10 µg/mL
1 x 40 µL
Detecting Antibody, 200 µg/mL
1 x 20 µL
Developing Solution
1 x 10 mL
Stop Solution
1 x 6 mL
Substrate Binding Solution
1 x 10 mL

Recommended products

DNA methylation plays an essential role in biological processess through repression of transcription, while DNA demethylation is necessary, on the other hand, for epigenetic reprogramming of these genes.

Key facts

Assay type
Enzyme activity
Reactive species
Mouse, Human
Assay time
3h
Assay Platform
Microplate reader

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

DNA methylation plays an essential role in biological processess through repression of transcription, while DNA demethylation is necessary, on the other hand, for epigenetic reprogramming of these genes. Active demethylation of DNA requires specific demethylase participation such as from MBD2.

DNA demethylase (total) Activity Quantification Assay Kit (ab113472) allows the user to measure total DNA demethylase activity/inhibition at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed for measuring total DNA demethylase activity/inhibition.

ab113472 is suitable for measuring DNA demethylase activity/inhibition from cultured cells/tissues or using purified enzyme/protein extracts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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1 product image

  • Functional Studies - DNA demethylase (total) Activity Quantification Assay Kit (ab113472), expandable thumbnail

    Functional Studies - DNA demethylase (total) Activity Quantification Assay Kit (ab113472)

    Nuclear extracts were prepared from MCF-7 cells using the Nuclear Extraction Kit (Nuclear Extraction Kit ab113474) and total DNA demethylase activity was measured using ab113472.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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