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AB156908

DNA demethylase (total) Activity Quantification Ultra Assay Kit

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(1 Publication)

Abcam's DNA demethylase (total) Activity Quantification Ultra Assay Kit (ab156908) is a complete set of essential components which enables an experimenter to measure total DNA demethylase activity/inhibition.
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Functional Studies - DNA demethylase (total) Activity Quantification Ultra Assay Kit (AB156908)
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Supplier Data

Functional Studies - DNA demethylase (total) Activity Quantification Ultra Assay Kit (AB156908)

Standard Curve

Assay control standard was added into the assay wells at different concentrations and then measured with Abcam's DNA demethylase (total) Activity Quantification Ultra Assay Kit (ab156908).

Key facts

Sample types

Nuclear Extracts, Cell culture extracts, Tissue Extracts

Results type

Quantitative

Assay time

4h

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's DNA demethylase (total) Activity Quantification Ultra Assay Kit (ab156908) is a complete set of essential components which enables an experimenter to measure total DNA demethylase activity/inhibition. The kit can be used with nuclear extracts from a broad range of species such as mammals and plants, in a variety of forms including, but not limited to, cultured cells and fresh tissues.

DNA demethylation is necessary for the epigenentic reprogramming of genes and is also directly involved in many important disease mechanisms such as tumor progression. Demethylation of DNA can either be passive or active, or a combination of both. Passive DNA demethylation usually takes place on newly synthesized DNA strands via DNMT1 during replication rounds. Active DNA demethylation mainly occurs by the removal of 5-methylcytosine through further modified cytosine bases which have been converted by TET enzyme-mediated oxidation. These oxidation products have been shown to be repaired by TDG, a glycosylase which is involved in base excision repair, or directly converted to cytosine by DNMT3A/ DNMT3B in oxidative states. It is proposed that DNA demethylation could also be initiated by deamination of 5-mC through candidate deaminases including AID and APOBEC1, which convert 5-mC to thymine. The resulting thymine could be repaired by BER initiated by a T-G mismatch glycosylase such as MBD4 or TDG. In addition, the 5-mC base can be directly removed in plants by the DME/ROS1 family of 5-mC DNA glycosylases, resulting in an abasic site that is repaired by the BER process.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental botany 66:5959-69 PubMed26116024

2015

AtROS1 overexpression provides evidence for epigenetic regulation of genes encoding enzymes of flavonoid biosynthesis and antioxidant pathways during salt stress in transgenic tobacco.

Applications

Unspecified application

Species

Unspecified reactive species

Poonam Bharti,Monika Mahajan,Ajay K Vishwakarma,Jyoti Bhardwaj,Sudesh Kumar Yadav
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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