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AB117139

DNA-Protein Binding Assay Kit (Colorimetric)

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(9 Publications)

DNA-Protein Binding Assay Kit (Colorimetric) (ab117139) allows the user to investigate direct in vitro protein-DNA interaction, using dsDNA of choice, within 3 hours. ab117139 is specifically suitable for detection of transcription factor activation in mammalian tissue and cell extracts.

Key facts

Detection method

Colorimetric

Assay time

3h

Assay Platform

Microplate reader

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Product details

Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. Identifying the genetic targets of DNA binding proteins and knowing the mechanishm of protein-DNA interaction is important for understanding cellular processes.

DNA-Protein Binding Assay Kit (Colorimetric) (ab117139) allows the user to investigate direct in vitro protein-DNA interaction, using dsDNA of choice, within 3 hours. ab117139 is specifically suitable for detection of transcription factor activation in mammalian tissue and cell extracts.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 9: PubMed38516887

2024

Characterization of HMGA2 variants expands the spectrum of Silver-Russell syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Avinaash V Maharaj,Emily Cottrell,Thatchawan Thanasupawat,Sjoerd D Joustra,Barbara Triggs-Raine,Masanobu Fujimoto,Sarina G Kant,Danielle van der Kaay,Agnes Clement-de Boers,Alice S Brooks,Gabriel Amador Aguirre,Irene Martín Del Estal,María Inmaculada Castilla de Cortázar Larrea,Ahmed Massoud,Hermine A van Duyvenvoorde,Christiaan De Bruin,Vivian Hwa,Thomas Klonisch,Sabine Hombach-Klonisch,Helen L Storr

Scientific reports 13:11776 PubMed37479820

2023

Phenotypic and molecular basis of SIX1 variants linked to non-syndromic deafness and atypical branchio-otic syndrome in South Korea.

Applications

Unspecified application

Species

Unspecified reactive species

Somin Lee,Yejin Yun,Ju Hyuen Cha,Jin Hee Han,Dae Hee Lee,Jae-Jin Song,Moo Kyun Park,Jun Ho Lee,Seung Ha Oh,Byung Yoon Choi,Sang-Yeon Lee

Frontiers in endocrinology 14:1177268 PubMed37396188

2023

Molecular characterization and re-interpretation of variants identified in Indian MODY subjects towards precision medicine.

Applications

Unspecified application

Species

Unspecified reactive species

Babu Kavitha,Sampathkumar Ranganathan,Sundaramoorthy Gopi,Umashankar Vetrivel,Nagarajan Hemavathy,Viswanathan Mohan,Venkatesan Radha

Physiologia plantarum 173:2376-2389 PubMed34687457

2021

Loss-of-function of ARABIDOPSIS F-BOX PROTEIN HYPERSENSITIVE TO ABA 1 enhances drought tolerance and delays germination.

Applications

Unspecified application

Species

Unspecified reactive species

Hani Kim,Eunyoung Song,Yeojin Kim,Eunsil Choi,Jihwan Hwang,Jae-Hoon Lee

Molecular cancer therapeutics 20:612-622 PubMed33277444

2020

The Histone Methyltransferase Gene G9A Is Regulated by Nuclear Receptor 4A1 in Alveolar Rhabdomyosarcoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rupesh Shrestha,Kumaravel Mohankumar,Un-Ho Jin,Gregory Martin,Stephen Safe

Neurology. Genetics 6:e407 PubMed32337334

2020

Novel EGR2 variant that associates with Charcot-Marie-Tooth disease when combined with lipopolysaccharide-induced TNF-α factor T49M polymorphism.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Empar Blanco-Cantó,Nikiben Patel,Sergio Velasco-Aviles,Angeles Casillas-Bajo,Juan Salas-Felipe,Alexandre García-Escrivá,Carmen Díaz-Marín,Hugo Cabedo

Proceedings of the National Academy of Sciences of the United States of America 115:E253-E262 PubMed29269391

2017

FoxP3 scanning mutagenesis reveals functional variegation and mild mutations with atypical autoimmune phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Ho-Keun Kwon,Hui-Min Chen,Diane Mathis,Christophe Benoist

Nature immunology 18:1238-1248 PubMed28892470

2017

Different molecular complexes that mediate transcriptional induction and repression by FoxP3.

Applications

Unspecified application

Species

Unspecified reactive species

Ho-Keun Kwon,Hui-Min Chen,Diane Mathis,Christophe Benoist

eLife 4:e06283 PubMed26067234

2015

MAF1 represses CDKN1A through a Pol III-dependent mechanism.

Applications

FuncS

Species

Unspecified reactive species

Yu-Ling Lee,Yuan-Ching Li,Chia-Hsin Su,Chun-Hui Chiao,I-Hsuan Lin,Ming-Ta Hsu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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