DNase I Assay Kit (Fluorometric)

DNase I Assay Kit (Fluorometric) (ab234056) allows for quantitative evaluation of DNase I activity of purified enzymes and their inhibitors as well as comparative examination of DNase I activity in biological samples. Enzyme activity is detected upon cleavage of a DNA Probe, which yields a fluorescent DNA product measured at Ex/Em = 651/681 nm. The limit of quantification (L.O.Q) is 178 fmoles of DNA probe cleaved per minute per mL.

This product is manufactured by BioVision, an Abcam company and was previously called K429 DNAse I Activity Assay Kit (Fluorometric). K429-100 is the same size as the 100 test size of ab234056.

Deoxyribonuclease I (DNase I) is an endonuclease that cleaves DNA phosphodiester bonds yielding 5′-phosphorylated and 3′-hydroxylated oligonucleotides. DNase I targets single-stranded DNA, double-stranded DNA, and chromatin in a non-specific manner. As an important player in cellular waste management, DNase I is normally secreted extracellularly to clear the system from circulating cell-free DNA, foreign DNA from food digestion or potential pathogens, and endogenous chromosomal DNA from apoptotic and necrotic cells. Abnormal DNase I activity occurs in association with a variety of cancers and auto-immune illnesses that exhibit elevated levels of cell-free DNA. Furthermore, DNase I has been therapeutically used in cystic fibrosis patients to degrade DNA and reduce sputum viscosity.