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AB113467

DNMT Activity Assay Kit (Colorimetric)

4

(2 Reviews)

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(19 Publications)

DNMT Activity Assay Kit (Colorimetric) ab113467 allows the user to colorimetrically measure DNA methyltransferase activity or inhibition at extremely fast speed on a 96-strip well microplate.
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Functional Studies - DNMT Activity Assay Kit (Colorimetric) (AB113467)
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Supplier Data

Functional Studies - DNMT Activity Assay Kit (Colorimetric) (AB113467)

Demonstration of high sensitivity and specificity of the DNMT activity assay achieved by using nuclear extracts with ab113467. Nuclear extracts were prepared from MCF-7 cells using ab113474.

Functional Studies - DNMT Activity Assay Kit (Colorimetric) (AB113467)
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Supplier Data

Functional Studies - DNMT Activity Assay Kit (Colorimetric) (AB113467)

Demonstration of high sensitivity and specificity of the DNMT activity/inhibition assay achieved by using recombinant DNMT1 with ab113467.

Key facts

Detection method

Colorimetric

Assay type

Enzyme activity (quantitative)

Assay time

3h 45m

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

DNMT Activity Assay Kit (Colorimetric) ab113467 allows the user to colorimetrically measure DNA methyltransferase activity or inhibition at extremely fast speed on a 96-strip well microplate.

The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT activity/inhibition experiment without the need for radioactivity or any special equipment.

DNMT assay protocol method
In the DNMT assay protocol, DNMT enzymes in a sample transfer methyl groups from Adomet to a DNMT DNA substrate coated onto microplate wells. The methylated DNA is bound with a 5-methylcytosine antibody. A secondary antibody binds the 5-methylcytosine antibody, and the enzyme-conjugated secondary antibody is detected using a chromogenic enzyme substrate.

DNMT assay protocol summary
- add samples, positive controls, and samples with inhibitor to wells
- incubate for 90-120 min, and wash wells three times with wash buffer
- add capture antibody, incubate for 60 min, and wash wells
- add detection antibody, incubate for 30 min, and wash wells
- add enhancer solution, incubate for 30 min, and wash wells
- add developer solution, incubate for 1-10 min
- add stop solution and analyze with a microplate reader

Other Notes
This product was previously called DNMT Activity Quantification Kit (Colorimetric).

DNMT (DNA methylases) is a family of enzymes responsible for the addition of methyl groups on the cytosine ring at the 5' position of a CpG dinucleotide. The DNMT family is required for the establishment and maintenance of DNA methylation patterns.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

See full target information DNA Methylase
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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Translational psychiatry 15:198 PubMed40506434

2025

Concentration-dependent effect of delta-9-tetrahydrocannabinol on epigenetic DNA modifiers in human peripheral blood mononuclear cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kerda Pulk,Kelli Somelar-Duracz,Mikk Rooden,Kaili Anier,Anti Kalda

Journal of cellular and molecular medicine 29:e70498 PubMed40159638

2025

Kaempferol Targets Global Epigenetic Modifiers to Impedes Growth and Migratory Ability of HeLa Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nazia Afroze,Shafiul Haque,Arif Hussain

Acta neuropathologica communications 12:40 PubMed38481314

2024

DNA hypomethylator phenotype reprograms glutamatergic network in receptor tyrosine kinase gene-mutated glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mio Harachi,Kenta Masui,Erika Shimizu,Kumiko Murakami,Hiromi Onizuka,Yoshihiro Muragaki,Takakazu Kawamata,Hisako Nakayama,Mariko Miyata,Takashi Komori,Webster K Cavenee,Paul S Mischel,Atsushi Kurata,Noriyuki Shibata

Life (Basel, Switzerland) 13: PubMed38137854

2023

Tacrolimus Maintains the Balance of Neutrophil Extracellular Traps by Inducing DNA Methylation of Neutrophils to Reduce Immune Rejection.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Xu,Ming Cai

Cells 12: PubMed36672198

2023

Skeletal Muscle DNA Methylation and mRNA Responses to a Bout of Higher versus Lower Load Resistance Exercise in Previously Trained Men.

Applications

Unspecified application

Species

Unspecified reactive species

Casey L Sexton,Joshua S Godwin,Mason C McIntosh,Bradley A Ruple,Shelby C Osburn,Blake R Hollingsworth,Nicholas J Kontos,Philip J Agostinelli,Andreas N Kavazis,Tim N Ziegenfuss,Hector L Lopez,Ryan Smith,Kaelin C Young,Varun B Dwaraka,Andrew D Frugé,Christopher B Mobley,Adam P Sharples,Michael D Roberts

Clinical epigenetics 14:89 PubMed35842682

2022

Psychostimulant-induced aberrant DNA methylation in an in vitro model of human peripheral blood mononuclear cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kaili Anier,Kelli Somelar,Külli Jaako,Margret Alttoa,Kerli Sikk,Raul Kokassaar,Kai Kisand,Anti Kalda

Frontiers in pharmacology 13:815646 PubMed35559231

2022

Modified Adenosines Sensitize Glioblastoma Cells to Temozolomide by Affecting DNA Methyltransferases.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Chiara Proto,Donatella Fiore,Chiara Piscopo,Chiara Laezza,Maurizio Bifulco,Patrizia Gazzerro

Biomedicines 10: PubMed35453616

2022

DNMT3B System Dysregulation Contributes to the Hypomethylated State in Ischaemic Human Hearts.

Applications

Unspecified application

Species

Unspecified reactive species

Estefanía Tarazón,Lorena Pérez-Carrillo,Isaac Giménez-Escamilla,María García-Manzanares,Luis Martínez-Dolz,Manuel Portolés,Esther Roselló-Lletí

Toxics 10: PubMed35202286

2022

DNA Methylation and Detoxification in the Earthworm Exposed to Cadmium and the DNA Demethylation Agent 5-aza-2'-deoxycytidine.

Applications

Unspecified application

Species

Unspecified reactive species

Gerhard P Aigner,Pamela Nenning,Birgit Fiechtner,Maja Šrut,Martina Höckner

Birth defects research 113:1470-1483 PubMed34668346

2021

Resveratrol ameliorates toxic effects of cadmium on placental development in mouse placenta and human trophoblast cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjie Wang,Guiying Liu,Xuelian Jiang,Guimei Wu
View all publications
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