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DNMT (DNA methylases) is a family of enzymes responsible for the addition of methyl groups on the cytosine ring at the 5' position of a CpG dinucleotide.

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Images

Functional Studies - DNMT Activity Assay Kit (Fluorometric) (AB113468), expandable thumbnail

Publications

Key facts

Detection method
Fluorescent
Assay type
Enzyme activity
Reactive species
Mouse, Human, Plants
Assay time
3h 45m

Associated Products

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What's included?

96 Test
Components
10X Wash Buffer
1 x 28 mL
8-Well Assay Strips (with Frame)
12 x 1 Unit
Adhesive Covering Film
1 x 1 Unit
Adomet, 50X
1 x 120 µL
Capture Antibody, 1000 µg/mL
1 x 10 µL
DNMT Assay Buffer
1 x 8 mL
DNMT Enzyme Control, 50 µg/mL
1 x 12 µL
Detection Antibody, 400 µg/mL
1 x 12 µL
Enhancer Solution
1 x 12 µL
Fluoro Developer
1 x 12 µL
Fluoro Diluter
1 x 8 mL
Fluoro Enhancer
1 x 12 µL

Recommended products

DNMT (DNA methylases) is a family of enzymes responsible for the addition of methyl groups on the cytosine ring at the 5' position of a CpG dinucleotide.

Key facts

Detection method
Fluorescent
Assay type
Enzyme activity
Reactive species
Mouse, Human, Plants
Assay time
3h 45m
Assay Platform
Microplate reader

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

DNMT (DNA methylases) is a family of enzymes responsible for the addition of methyl groups on the cytosine ring at the 5' position of a CpG dinucleotide. The DNMT family is required for the establishment and maintenance of DNA methylation patterns.
DNMT Activity Quantification Kit (Fluorometric) (ab113468) allows the user to fluorometrically measure DNA methyltransferase activity or inhibition at extremely fast speed on a 96-stripwell microplate. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT activity/inhibition experiment without the need for radioactivity or any special equipment.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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1 product image

  • Functional Studies - DNMT Activity Assay Kit (Fluorometric) (ab113468), expandable thumbnail

    Functional Studies - DNMT Activity Assay Kit (Fluorometric) (ab113468)

    Demonstration of high sensitivity and specificity achieved by using recombinant DNMT1 with ab113468.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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