AB113468
DNMT Activity Assay Kit (Fluorometric)
Be the first to review this product! Submit a review
|
(1 Publication)
DNMT (DNA methylases) is a family of enzymes responsible for the addition of methyl groups on the cytosine ring at the 5' position of a CpG dinucleotide.
1 Images
- FuncS
Supplier Data
Functional Studies - DNMT Activity Assay Kit (Fluorometric) (AB113468)
Demonstration of high sensitivity and specificity achieved by using recombinant DNMT1 with ab113468.
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
Product details
DNMT (DNA methylases) is a family of enzymes responsible for the addition of methyl groups on the cytosine ring at the 5' position of a CpG dinucleotide. The DNMT family is required for the establishment and maintenance of DNA methylation patterns.
DNMT Activity Quantification Kit (Fluorometric) (ab113468) allows the user to fluorometrically measure DNA methyltransferase activity or inhibition at extremely fast speed on a 96-stripwell microplate. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT activity/inhibition experiment without the need for radioactivity or any special equipment.
DNMT Activity Quantification Kit (Fluorometric) (ab113468) allows the user to fluorometrically measure DNA methyltransferase activity or inhibition at extremely fast speed on a 96-stripwell microplate. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT activity/inhibition experiment without the need for radioactivity or any special equipment.
What's included?
{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab113468-96Test", "assetComponentDetails": [ { "size":"1 x 12 µL", "name":"Fluoro Enhancer", "number":"AB113468-CMP11" }, { "size":"1 x 28 mL", "name":"10X Wash Buffer", "number":"AB113468-CMP02" }, { "size":"1 x 8 mL", "name":"DNMT Assay Buffer", "number":"AB113468-CMP06" }, { "size":"1 x 8 mL", "name":"Fluoro Diluter", "number":"AB113468-CMP10" }, { "size":"1 x 12 µL", "name":"Enhancer Solution", "number":"AB113468-CMP08" }, { "size":"12 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB113468-CMP01" }, { "size":"1 x 10 µL", "name":"Capture Antibody, 1000 µg/mL", "number":"AB113468-CMP04" }, { "size":"1 x 1 Unit", "name":"Adhesive Covering Film", "number":"AB113468-CMP12" }, { "size":"1 x 12 µL", "name":"DNMT Enzyme Control, 50 µg/mL", "number":"AB113468-CMP07" }, { "size":"1 x 12 µL", "name":"Fluoro Developer", "number":"AB113468-CMP09" }, { "size":"1 x 12 µL", "name":"Detection Antibody, 400 µg/mL", "number":"AB113468-CMP05" }, { "size":"1 x 120 µL", "name":"Adomet, 50X", "number":"AB113468-CMP03" } ] }, "48Test": { "sellingSize": "48 Test", "publicAssetCode":"ab113468-48Test", "assetComponentDetails": [ { "size":"1 x 4 mL", "name":"DNMT Assay Buffer", "number":"AB113468-CMP06", "productcode":"" }, { "size":"1 x 14 mL", "name":"10X Wash Buffer", "number":"AB113468-CMP02", "productcode":"" }, { "size":"1 x 6 µL", "name":"Fluoro Developer", "number":"AB113468-CMP09", "productcode":"" }, { "size":"1 x 4 mL", "name":"Fluoro Diluter", "number":"AB113468-CMP10", "productcode":"" }, { "size":"6 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB113468-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Adhesive Covering Film", "number":"AB113468-CMP12", "productcode":"" }, { "size":"1 x 6 µL", "name":"DNMT Enzyme Control, 50 µg/mL", "number":"AB113468-CMP07", "productcode":"" }, { "size":"1 x 6 µL", "name":"Detection Antibody, 400 µg/mL", "number":"AB113468-CMP05", "productcode":"" }, { "size":"1 x 5 µL", "name":"Capture Antibody, 1000 µg/mL", "number":"AB113468-CMP04", "productcode":"" }, { "size":"1 x 6 µL", "name":"Fluoro Enhancer", "number":"AB113468-CMP11", "productcode":"" }, { "size":"1 x 6 µL", "name":"Enhancer Solution", "number":"AB113468-CMP08", "productcode":"" }, { "size":"1 x 60 µL", "name":"Adomet, 50X", "number":"AB113468-CMP03", "productcode":"" } ] } } }
Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Nature structural & molecular biology 25:951-957 PubMed30275516
2018
DNA G-quadruplex structures mold the DNA methylome.
Applications
Unspecified application
Species
Unspecified reactive species
Shi-Qing Mao,Avazeh T Ghanbarian,Jochen Spiegel,Sergio Martínez Cuesta,Dario Beraldi,Marco Di Antonio,Giovanni Marsico,Robert Hänsel-Hertsch,David Tannahill,Shankar Balasubramanian
Product promise
We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com