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DNMT (DNA methylases) is a family of enzymes responsible for the addition of methyl groups on the cytosine ring at the 5' position of a CpG dinucleotide.

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Detection method

Fluorescent

Assay type

Enzyme activity

Reactive species

Mouse, Human, Plants

Assay time

3h 45m

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Images

What's included?

96 Test
Components
10X Wash Buffer
1 x 28 mL
8-Well Assay Strips (with Frame)
12 x 1 Unit
Adhesive Covering Film
1 x 1 Unit
Adomet, 50X
1 x 120 µL
Capture Antibody, 1000 µg/mL
1 x 10 µL
DNMT Assay Buffer
1 x 8 mL
DNMT Enzyme Control, 50 µg/mL
1 x 12 µL
Detection Antibody, 400 µg/mL
1 x 12 µL
Enhancer Solution
1 x 12 µL
Fluoro Developer
1 x 12 µL
Fluoro Diluter
1 x 8 mL
Fluoro Enhancer
1 x 12 µL

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DNMT (DNA methylases) is a family of enzymes responsible for the addition of methyl groups on the cytosine ring at the 5' position of a CpG dinucleotide.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Detection method

Fluorescent

Assay type

Enzyme activity

Reactive species

Mouse, Human, Plants

Assay time

3h 45m

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

DNMT (DNA methylases) is a family of enzymes responsible for the addition of methyl groups on the cytosine ring at the 5' position of a CpG dinucleotide. The DNMT family is required for the establishment and maintenance of DNA methylation patterns.
DNMT Activity Quantification Kit (Fluorometric) (ab113468) allows the user to fluorometrically measure DNA methyltransferase activity or inhibition at extremely fast speed on a 96-stripwell microplate. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT activity/inhibition experiment without the need for radioactivity or any special equipment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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