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AB113469

DNMT1 Assay Kit

4

(1 Review)

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(8 Publications)

DNMT1 (DNA (cytosine-5)-methyltransferase 1) belongs to the DNMT family, responsible for methylation of CpG islands in the genome.

View Alternative Names

AIM, CXXC9, DNMT, DNMT1, DNA (cytosine-5)-methyltransferase 1, Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, MCMT, DNA MTase HsaI, M.HsaI

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Functional Studies - DNMT1 Assay Kit (AB113469)
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Supplier Data

Functional Studies - DNMT1 Assay Kit (AB113469)

Nuclear extracts were prepared from MCF-7 cells using the Nuclear Extraction Kit (ab113474) and DNMT1 activity was measured using ab113469. DNMT3A and DNMT3B activities were measured using ab113470 DNMT3A Assay Kit and ab113471 EpiSeeker DNMT3B Assay Kit.

Key facts

Sample types

Tissue Extracts, Cell Lysate

Results type

Quantitative

Assay time

3h

Assay Platform

Microplate reader

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Reactivity data

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Product details

DNMT1 (DNA (cytosine-5)-methyltransferase 1) belongs to the DNMT family, responsible for methylation of CpG islands in the genome. DNMT1 preferably methylates cytosines located in hemimethylated DNA and it associates with DNA replication sites in S-phase.

DNMT1 Assay Kit (ab113469) allows the user to quickly and consistently measure the amount of DNMT1. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT1 assay experiment without the need for electrophoresis and transfer process. ab113469 is suitable for measuring DNMT amounts quantitatively from fresh tissue and cultured cells of human and mouse.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed : 24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed : 24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed : 24623306). Promotes tumor growth (PubMed : 24623306).
See full target information DNMT1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 21:276 PubMed37088830

2023

The activation of mTOR signalling modulates DNA methylation by enhancing DNMT1 translation in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mengke Chen,Yi Fang,Meinong Liang,Ning Zhang,Xinyue Zhang,Lixia Xu,Xuxin Ren,Qingfeng Zhang,Yufeng Zhou,Sui Peng,Jun Yu,Judeng Zeng,Xiaoxing Li

Disease markers 2022:8426384 PubMed35996715

2022

Expression and Clinical Significance of HER2 Gene and DNMT1 in Non-Small-Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Weiying Diao,Chenglong Ding,Boyang Yuan,Zan Li,Na Sun,Jiabin Huang

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:14558-14571 PubMed32946656

2020

Sennoside A prevents liver fibrosis by binding DNMT1 and suppressing DNMT1-mediated PTEN hypermethylation in HSC activation and proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Zhu,Changsheng He,Huizi Zhao,Wenjuan Jiang,Songbing Xu,Jun Li,Taotao Ma,Cheng Huang

Microbiome 8:108 PubMed32678024

2020

Bacteria pathogens drive host colonic epithelial cell promoter hypermethylation of tumor suppressor genes in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxuan Xia,William Ka Kei Wu,Sunny Hei Wong,Dabin Liu,Thomas Ngai Yeung Kwong,Geicho Nakatsu,Pearlly S Yan,Yu-Ming Chuang,Michael Wing-Yan Chan,Olabisi Oluwabukola Coker,Zigui Chen,Yun Kit Yeoh,Liuyang Zhao,Xiansong Wang,Wing Yin Cheng,Matthew Tak Vai Chan,Paul Kay Sheung Chan,Joseph Jao Yiu Sung,Maggie Haitian Wang,Jun Yu

Food and chemical toxicology : an international jo 124:450-461 PubMed30557669

2018

Single nanomolar doxorubicin exposure triggers compensatory mitochondrial responses in H9c2 cardiomyoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Luciana L Ferreira,Teresa Cunha-Oliveira,Caroline D Veloso,Cláudio F Costa,Kendall B Wallace,Paulo J Oliveira

Lipids 53:581-588 PubMed30203512

2018

Dietary Unsaturated Fatty Acids Modulate Maternal Dyslipidemia-Induced DNA Methylation and Histone Acetylation in Placenta and Fetal Liver in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Breetha Ramaiyan,Ramaprasad Ravichandra Talahalli

Molecular medicine reports 16:9181-9188 PubMed29039510

2017

Homocysteine‑induced oxidative stress through TLR4/NF‑κB/DNMT1‑mediated LOX‑1 DNA methylation in endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng-Chao Ma,Yin-Ju Hao,Yun Jiao,Yan-Hua Wang,Ling-Bo Xu,Cai-Yan Mao,Xiao-Ling Yang,An-Ning Yang,Jue Tian,Ming-Hao Zhang,Shao-Ju Jin,Hua Xu,Yi-Deng Jiang,Hui-Ping Zhang

Scientific reports 7:2807 PubMed28584243

2017

NKX6.3 Regulates Reactive Oxygen Species Production by Suppressing NF-kB and DNMT1 Activities in Gastric Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Hwan Yoon,Olga Kim,Suk Woo Nam,Jung Young Lee,Won Sang Park
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