DNMT1 (DNA (cytosine-5)-methyltransferase 1) belongs to the DNMT family, responsible for methylation of CpG islands in the genome.
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- Journal of translational medicine 21:2762023The activation of mTOR signalling modulates DNA methylation by enhancing DNMT1 translation in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengke Chen,Yi Fang,Meinong Liang,Ning Zhang,Xinyue Zhang,Lixia Xu,Xuxin Ren,Qingfeng Zhang,Yufeng Zhou,Sui Peng,Jun Yu,Judeng Zeng,Xiaoxing LiPubMed 37088830
- Disease markers 2022:84263842022Expression and Clinical Significance of HER2 Gene and DNMT1 in Non-Small-Cell Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiying Diao,Chenglong Ding,Boyang Yuan,Zan Li,Na Sun,Jiabin HuangPubMed 35996715
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:14558-145712020Sennoside A prevents liver fibrosis by binding DNMT1 and suppressing DNMT1-mediated PTEN hypermethylation in HSC activation and proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong Zhu,Changsheng He,Huizi Zhao,Wenjuan Jiang,Songbing Xu,Jun Li,Taotao Ma,Cheng HuangPubMed 32946656
- Microbiome 8:1082020Bacteria pathogens drive host colonic epithelial cell promoter hypermethylation of tumor suppressor genes in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoxuan Xia et. al.PubMed 32678024
- Food and chemical toxicology : an international jo 124:450-4612018Single nanomolar doxorubicin exposure triggers compensatory mitochondrial responses in H9c2 cardiomyoblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuciana L Ferreira et. al.PubMed 30557669
- Lipids 53:581-5882018Dietary Unsaturated Fatty Acids Modulate Maternal Dyslipidemia-Induced DNA Methylation and Histone Acetylation in Placenta and Fetal Liver in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBreetha Ramaiyan,Ramaprasad Ravichandra TalahalliPubMed 30203512
- Molecular medicine reports 16:9181-91882017Homocysteine‑induced oxidative stress through TLR4/NF‑κB/DNMT1‑mediated LOX‑1 DNA methylation in endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSheng-Chao Ma et. al.PubMed 29039510
- Scientific reports 7:28072017NKX6.3 Regulates Reactive Oxygen Species Production by Suppressing NF-kB and DNMT1 Activities in Gastric Epithelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJung Hwan Yoon et. al.PubMed 28584243
Key facts
- Sample types
- Tissue Extracts, Cell Lysate
- Assay type
- Quantitative
- Reactive species
- Mouse, Human
- Assay time
- 3h
Target data
Function
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). Promotes tumor growth (PubMed:24623306).
Alternative names
AIM, CXXC9, DNMT, DNMT1, DNA (cytosine-5)-methyltransferase 1, Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, MCMT, DNA MTase HsaI, M.HsaI
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DNMT1 (DNA (cytosine-5)-methyltransferase 1) belongs to the DNMT family, responsible for methylation of CpG islands in the genome.
Key facts
- Sample types
- Tissue Extracts, Cell Lysate
- Assay type
- Quantitative
- Reactive species
- Mouse, Human
- Assay time
- 3h
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
DNMT1 (DNA (cytosine-5)-methyltransferase 1) belongs to the DNMT family, responsible for methylation of CpG islands in the genome. DNMT1 preferably methylates cytosines located in hemimethylated DNA and it associates with DNA replication sites in S-phase.
DNMT1 Assay Kit (ab113469) allows the user to quickly and consistently measure the amount of DNMT1. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT1 assay experiment without the need for electrophoresis and transfer process. ab113469 is suitable for measuring DNMT amounts quantitatively from fresh tissue and cultured cells of human and mouse.
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Functional Studies - DNMT1 Assay Kit (ab113469)
Nuclear extracts were prepared from MCF-7 cells using the Nuclear Extraction Kit (Nuclear Extraction Kit ab113474) and DNMT1 activity was measured using ab113469. DNMT3A and DNMT3B activities were measured using DNMT3A Assay Kit ab113470 DNMT3A Assay Kit and DNMT3B Assay Kit ab113471 EpiSeeker DNMT3B Assay Kit.
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