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AB113470

DNMT3A Assay Kit

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(3 Publications)

DNMT3A (DNA (cytosine-5)-methyltransferase 3A) belongs to the DNMT family, and it is required for genome wide de novo methylation and it is essential for the establishment of DNA methylation patterns during development.

View Alternative Names

DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, Cysteine methyltransferase DNMT3A, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, M.HsaIIIA, DNMT3A

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Functional Studies - DNMT3A Assay Kit (AB113470)
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Supplier Data

Functional Studies - DNMT3A Assay Kit (AB113470)

Nuclear extracts were prepared from MCF-7 cells using the Nuclear Extraction Kit (ab113474) and DNMT3A activity was measured using ab113470. DNMT1 and DNMT3B activities were measured using ab113469 DNMT1 Assay Kit and ab113471 DNMT3B Assay Kit.

Key facts

Results type

Quantitative

Assay time

3h

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

DNMT3A (DNA (cytosine-5)-methyltransferase 3A) belongs to the DNMT family, and it is required for genome wide de novo methylation and it is essential for the establishment of DNA methylation patterns during development.

DNMT3A Assay Kit (ab113470) allows the user to quickly and consistently measure the amount of DNMT3A. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT3A assay experiment. ab113470 is suitable for measuring DNMT amounts quantitatively from fresh tissue and cultured cells of human and mouse.

Not sure if this is the right product for you? Check out our EpiSeeker DNMT Product Guide for help.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development (PubMed : 12138111, PubMed : 16357870, PubMed : 30478443). DNA methylation is coordinated with methylation of histones (PubMed : 12138111, PubMed : 16357870, PubMed : 30478443). It modifies DNA in a non-processive manner and also methylates non-CpG sites (PubMed : 12138111, PubMed : 16357870, PubMed : 30478443). May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1 (By similarity). Plays a role in paternal and maternal imprinting (By similarity). Required for methylation of most imprinted loci in germ cells (By similarity). Acts as a transcriptional corepressor for ZBTB18 (By similarity). Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites (By similarity). Can actively repress transcription through the recruitment of HDAC activity (By similarity). Also has weak auto-methylation activity on Cys-710 in absence of DNA (By similarity).
See full target information DNMT3A
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 15: PubMed40427627

2025

Uncovering a Novel Role of ROR1 in the Epigenetic Regulation of Tumor Suppressor Gene CREB3L1 in Triple-Negative Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria L Reed,Eric Lalu,Leena Yoon,Norman Fultang,Bela Peethambaran

Microbiome 8:108 PubMed32678024

2020

Bacteria pathogens drive host colonic epithelial cell promoter hypermethylation of tumor suppressor genes in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxuan Xia,William Ka Kei Wu,Sunny Hei Wong,Dabin Liu,Thomas Ngai Yeung Kwong,Geicho Nakatsu,Pearlly S Yan,Yu-Ming Chuang,Michael Wing-Yan Chan,Olabisi Oluwabukola Coker,Zigui Chen,Yun Kit Yeoh,Liuyang Zhao,Xiansong Wang,Wing Yin Cheng,Matthew Tak Vai Chan,Paul Kay Sheung Chan,Joseph Jao Yiu Sung,Maggie Haitian Wang,Jun Yu

Acta neuropathologica communications 4:46 PubMed27142428

2016

Dorsal root ganglia in Friedreich ataxia: satellite cell proliferation and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Arnulf H Koeppen,R Liane Ramirez,Alyssa B Becker,Joseph E Mazurkiewicz
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