JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB113471

DNMT3B Assay Kit

Be the first to review this product! Submit a review

|

(4 Publications)

DNMT3B (DNA (cytosine-5)-methyltransferase 3B) belongs to the DNMT family, it is required for genome de novo methylation and it is essential for establishment of DNA methylation patterns during development.

View Alternative Names

DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, M.HsaIIIB, DNMT3B

1 Images
Functional Studies - DNMT3B Assay Kit (AB113471)
  • FuncS

Supplier Data

Functional Studies - DNMT3B Assay Kit (AB113471)

Nuclear extracts were prepared from MCF-7 cells using the Nuclear Extraction Kit (ab113474) and DNMT3B activity was measured using ab113471. DNMT1 and DNMT3A activities were measured using ab113469 DNMT1 Assay Kit and ab113470 DNMT3A Assay Kit.

Key facts

Assay time

3h

Assay Platform

Microplate reader

style
left-column

Complementary Products

Assay Kits

AB113467

DNMT Activity Assay Kit (Colorimetric)

Functional Studies - DNMT Activity Assay Kit (Colorimetric) (AB113467)

  • 4
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Sample Prep & Detection Kits

AB113474

Nuclear Extraction Kit

Functional Studies - Nuclear Extraction Kit (AB113474)

  • 5
  • 3
Assay Kits

AB113469

DNMT1 Assay Kit

Functional Studies - DNMT1 Assay Kit (AB113469)

  • 4
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

DNMT3B (DNA (cytosine-5)-methyltransferase 3B) belongs to the DNMT family, it is required for genome de novo methylation and it is essential for establishment of DNA methylation patterns during development.

DNMT3B Assay Kit allows the user to quickly and consistently measure the amount of DNMT3B. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT3B assay experiment. DNMT3B Assay Kit is suitable for quantitative measurement of DNMT3B amounts from fresh tissue and cultured cells of human and mouse.

style
left-column

What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab113471-96Test", "assetComponentDetails": [ { "size":"1 x 20 µL", "name":"Detection Antibody, 200 µg/mL", "number":"AB113471-CMP06" }, { "size":"1 x 30 µL", "name":"DNMT3B Standard, 20 µg/mL", "number":"AB113471-CMP08" }, { "size":"1 x 16 µL", "name":"Capture Antibody, 100 µg/mL", "number":"AB113471-CMP04" }, { "size":"1 x 25 mL", "name":"10X Wash Buffer", "number":"AB113471-CMP03" }, { "size":"1 x 20 mL", "name":"Blocking Buffer", "number":"AB113471-CMP02" }, { "size":"1 x 10 mL", "name":"Assay Buffer", "number":"AB113471-CMP05" }, { "size":"12 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB113471-CMP01" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB113471-CMP09" }, { "size":"1 x 12 mL", "name":"Developing Solution", "number":"AB113471-CMP07" } ] }, "48Test": { "sellingSize": "48 Test", "publicAssetCode":"ab113471-48Test", "assetComponentDetails": [ { "size":"1 x 10 µL", "name":"Detection Antibody, 200 µg/mL", "number":"AB113471-CMP06", "productcode":"" }, { "size":"6 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB113471-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"10X Wash Buffer", "number":"AB113471-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"Blocking Buffer", "number":"AB113471-CMP02", "productcode":"" }, { "size":"1 x 8 µL", "name":"Capture Antibody, 100 µg/mL", "number":"AB113471-CMP04", "productcode":"" }, { "size":"1 x 5 mL", "name":"Assay Buffer", "number":"AB113471-CMP05", "productcode":"" }, { "size":"1 x 16 µL", "name":"DNMT3B Standard, 20 µg/mL", "number":"AB113471-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Stop Solution", "number":"AB113471-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Developing Solution", "number":"AB113471-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Functions as a transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells (PubMed : 27153398).
See full target information DNMT3B
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 15: PubMed40427627

2025

Uncovering a Novel Role of ROR1 in the Epigenetic Regulation of Tumor Suppressor Gene CREB3L1 in Triple-Negative Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria L Reed,Eric Lalu,Leena Yoon,Norman Fultang,Bela Peethambaran

Biomedicines 10: PubMed35453616

2022

DNMT3B System Dysregulation Contributes to the Hypomethylated State in Ischaemic Human Hearts.

Applications

Unspecified application

Species

Unspecified reactive species

Estefanía Tarazón,Lorena Pérez-Carrillo,Isaac Giménez-Escamilla,María García-Manzanares,Luis Martínez-Dolz,Manuel Portolés,Esther Roselló-Lletí

Microbiome 8:108 PubMed32678024

2020

Bacteria pathogens drive host colonic epithelial cell promoter hypermethylation of tumor suppressor genes in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxuan Xia,William Ka Kei Wu,Sunny Hei Wong,Dabin Liu,Thomas Ngai Yeung Kwong,Geicho Nakatsu,Pearlly S Yan,Yu-Ming Chuang,Michael Wing-Yan Chan,Olabisi Oluwabukola Coker,Zigui Chen,Yun Kit Yeoh,Liuyang Zhao,Xiansong Wang,Wing Yin Cheng,Matthew Tak Vai Chan,Paul Kay Sheung Chan,Joseph Jao Yiu Sung,Maggie Haitian Wang,Jun Yu

Theranostics 9:6256-6268 PubMed31534549

2019

Prostaglandin E induces DNA hypermethylation in gastric cancer and .

Applications

Unspecified application

Species

Unspecified reactive species

Chi Chun Wong,Wei Kang,Jiaying Xu,Yun Qian,Simson Tsz Yat Luk,Huarong Chen,Weilin Li,Liuyang Zhao,Xiaoming Zhang,Phlip Wy Chiu,Enders Kw Ng,Jun Yu
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com