DPPH Antioxidant Assay Kit (Colorimetric)

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

DPPH also known as 11-diphenyl-2-picrylhydrazyl is a stable free radical. It has a molecular mass of 394.32 g/mol. Scientists often use it in its radical form which presents as a deep violet color. This color changes upon reduction making it an excellent marker for antioxidant activity. While DPPH is not a biological protein it is extensively used in vitro to evaluate the free radical scavenging activities of antioxidants.

Biological function summary

In vitro antioxidant assays such as the DPPH assay measure how substances can neutralize free radicals. These assays use the DPPH molecule as a tool to quantify antioxidant activity in different samples. The DPPH assay protocol involves observing the change in color from deep violet to a pale yellow as antioxidants in the sample reduce DPPH radicals to non-radical DPPH-H form. The degree of color change reflects the sample's antioxidant capacity.

Pathways

DPPH assays help in understanding oxidative stress pathways by measuring the antioxidants' ability to counteract free radicals. These assays relate to pathways involving the oxidative stress response such as the Nrf2 signaling pathway which modulates the expression of antioxidant proteins. Although the DPPH radical itself does not participate in biological pathways its use in assays helps track activities alongside key proteins like glutathione.

Associated diseases and disorders

Oxidative stress plays a significant role in chronic inflammation and cardiovascular diseases. Antioxidant assessments using the DPPH test help evaluate how certain compounds might prevent or mitigate these conditions by neutralizing harmful free radicals. The assay indirectly links to disease mechanisms influenced by proteins like NADPH oxidase which generates oxidative stress associated with these diseases.